Selective extraction of n-butylidenephthalide from Angelica sinensis (Danggui) by using functionalized iron oxide magnetic nanoparticles as trapping probes

Abstract

n-Butylidenephthalide (BP) is an active compound in Angelica sinensis (Danggui) and a potential anti-cancer drug.

Riboflavin immobilized Fe 3 O 4 magnetic nanoparticles carried with n -butylidenephthalide as targeting-based anticancer agents

“…The binding capacity of RFMP onto Fe 3 O 4 MNPs at 530 nm rinsed with the solution from unbound RFMP was estimated to be $161.10 ± 3. 10 nmol mg À1 is also shown in Supplementary Figure S1, This result was similar to that of a previous study [44]. and MEM were used as solvents to examine the stability of RFMP on the as-prepared Fe 3 O 4 @RFMP in this complex medium.…”

“…RFMP was used to trap BP on Fe 3 O 4 MNPs. Thus, RFMP (0.2 mL, 10 À4 M) was initially immobilized on the surface of the as-prepared Fe 3 O 4 MNPs ($0.1 mg) through metal-phosphate chelation under microwave-heating (power: 540 W) for 3 min [44]. The as-prepared MNPs were rinsed to remove unbound species.…”

“…The as-prepared MNPs were rinsed to remove unbound species. The K d of Fe 3 O 4 @RFMP MNPs was estimated as $6.14 Â 10 À6 M [44]. Supplementary Figure S1 shows the fluorescence spectra (k ex ¼ 450 nm) of the supernatants containing RFMP obtained before and after incubation with Fe 3 O 4 MNPs.…”

“…For instance, Fe 3 O 4 magnetic NPs (MNPs) have good biocompability and are top choices in assisting drug delivery [43]. We previously demonstrated the feasibility of using Fe 3 O 4 MNPs immobilized with riboflavin-5 0 -phosphate (RFMP) as affinity probes to trap BP from the complex A. sinensis extract [44]. RFMP is an RF derivative containing a phosphate and can be immobilized on the surface of Fe 3 O 4 MNPs through Fe-phosphate chelation with a dissociation constant (…”

“…In addition, RFMP contains polycyclic aromatic rings and can interact with BP through p-p interactions, wherein K d between RFMP and BP was estimated to be $6.73 Â 10 -5 M [44]. In this study, we immobilized RFMP on the surface of Fe 3 O 4 MNPs and employed the aromatic ring on RFMP to interact with BP.…”