Selective extraction of phosphatidylcholine from lecithin by supercritical carbon dioxide/ethanol mixture

Abstract

Selective extraction of phosphatidylcholine (PC) from deoiled soybean lecithin using supercritical fluid (SCF) mixtures of carbon dioxide (CO 2 ) and ethanol was studied at moderate pressures. Temperature was varied between 60 and 80°C at pressures of 17.2 and 20.7 MPa. Ethanol was added as co-solvent to supercritical CO 2 at the levels of 10 and 12.5 wt%. Constant rate of extraction of the individual phospholipids (PL) was observed for 150 min during which the extractions were carried out. Pressure and ethanol fraction had a positive effect on the selective extraction of PC, whereas temperature had a negative effect. Under all the conditions studied, the extracts were mainly composed of PC while the extraction of the other PL was very low. Extraction at 60°C and 20.7 MPa with 10 wt% ethanol/90 wt% CO 2 SCF mixture resulted in 95% selectivity to PC.

Topical delivery of ocular therapeutics: carrier systems and physical methods

“…These emulsifiers are naturally occurring and show an affinity to cellular membranes because they contain a mixture of phospholipids, which are the molecules that comprise the cellular membrane. Phosphatidylcholine is the major phospholipid found in lecithins, and it is neutrally charged . Therefore, nanoemulsions produced with this surfactant as the major component generate nanoemulsions with a zeta potential that ranges from neutral to negative .…”

Analytical scale supercritical fluid fractionation and identification of single polar lipids from deoiled soybean lecithin

“…In another paper [22], these workers used an on-line SFE/supercritical fluid chromatograph (SFC) system to fractionate the deoiled PL extracts from soybean flakes. Recently, Teberikler et al [23] selectively extracted PC from deoiled soybean lecithin using CO 2 /10% ethanol at a moderate pressure and temperature.…”

Soybean-based Surfactants and Their Applications

“…Chromatographic separation is another way to prepare pure fractions of PLs. The drawback of this method is that a large volume of solvent is used, and thus it is cost ineffective [Teberikler et al, 2001].…”

“…Supercritical carbon dioxide (SC-CO2) is an excellent solvent for nonpolar lipids such as triglycerides and fatty acids while PLs have limited solubility in it [Teberikler, 2001]. It has been used as an alternative to organic solvents [Nasir et al, 2007].…”