Selective extraction of pyrethroid pesticide residues from milk by solid-matrix dispersion

Muccio, Alfonso Di; Pelosi, Patrizia; Barbini, Danilo Attard; Generali, Tiziana; Ausili, Antonella; Vergori, Fabio

doi:10.1016/s0021-9673(96)01005-9

Cited by 62 publications

(23 citation statements)

References 9 publications

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“…Pyrethroids (PYR), the synthetic analogues of natural compounds derived from chrysanthemum flowers [1], are among the most potent insecticides and are widely used for insect pest control in fruits, vegetables, and crops [2], as well as to control ecto-and endo-parasites on animals [3]. PYRs can contaminate food products; possible sources of contamination include: (i) food products containing high levels of PYR residues from post-harvest treatment; (ii) food products manufactured from plant materials that have been treated with insecticides during the growing season; (iii) the use of insecticides directly on animals; and (iv) the use of insecticides in stables and in milk processing factories [3].…”

Section: Introductionmentioning

confidence: 99%

“…PYRs can contaminate food products; possible sources of contamination include: (i) food products containing high levels of PYR residues from post-harvest treatment; (ii) food products manufactured from plant materials that have been treated with insecticides during the growing season; (iii) the use of insecticides directly on animals; and (iv) the use of insecticides in stables and in milk processing factories [3].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of pyrethroids from pesticide residues in porcine muscle and pasteurized milk using GC

Khay

El‐Aty

Choi

et al. 2009

J of Separation Science

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The principal goal of this work was to develop an efficient method for the simultaneous determination of four pyrethroid (PYR) insecticides, cyfluthrin, cyhalothrin, cypermethrin, and deltamethrin, in porcine muscle and pasteurized milk using liquid-liquid extraction (LLE). Sample extraction was carried out with and without additional column cleanup procedures, and the final determination was made using GC with electron-capture detector (ECD). The pesticide identity was confirmed using GC-MS in the SIM mode. Since there were minor differences between the extraction procedures, extraction without the additional cleanup procedure was used throughout the work. The method was validated by fortifying blank samples with half, two, and four times the maximum residue limit (MRL) of each PYR. The average recoveries (n = 6) ranged from 83.5 to 99.2% and 82.9 to 109% in porcine muscle and pasteurized milk, respectively. The repeatability of measurements expressed as RSDs, was in the range of 1.7-11.9 and 1.5-10.3% in porcine muscle and pasteurized milk, respectively. The LODs ranged from 3.3 to 9 and 3 to 8.1 ppm, whereas the LOQs ranged from 10 to 27.4 and 9 to 24.6 ppm, in porcine muscle and pasteurized milk, respectively. The applicability of the method was demonstrated by analyzing real samples collected from major cities in the Republic of Korea. No residues of the selected pesticides were detected in any of the samples.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of pyrethroids from pesticide residues in porcine muscle and pasteurized milk using GC

Khay

El‐Aty

Choi

et al. 2009

J of Separation Science

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“…Various clean-up approaches are involved to eliminate the matrix effect in milk samples (Tölgyesi et al, 2012;Xia et al, 2010, Martins et al, 2013. Especially, certain clean-up procedures were successfully employed for eliminating the matrix effect during the analysis of pesticide residues in milk samples (Spinks et al, 2001;Di Muccio et al, 1997;Di Muccio et al, 1996). Trace amounts of DDT in milk has to be dislodged by solvent extraction.…”

Section: Matrix Effect and Clean-up Proceduresmentioning

confidence: 99%

Development of ELISA kit for the assay of ichlorodiphenyltrichloroethane in milk and milk products

Kantharimuthu¹,

Balaraman²,

Sivanesan³

2016

Afr. J. Food Sci.

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Immunoassay of pesticide residues in foods is gaining importance in the past two decades. The objective of the present work is to develop an enzyme -linked immunosorbent assay (ELISA) kit for the assay of dichlorodiphenyltrichloroethane (DDT) in milk and milk products. Here, an ELISA method was developed using rabbit polyclonal antibodies against a hapten, DDT-OH for the detection of organochloride pesticide DDT in milk and milk products. The immunogen for raising the antiserum was prepared from BIS (4-chlorophenyl) ethanol and succinic anhydride coupled with bovine serum albumin (BSA) One ml of immunogen was inoculated subcutaneously in multiple sites of thigh region in rabbits following three boosters at weekly intervals and the antiserum was harvested after the second booster. The ELISA response of the anti DDT antiserum, diluted and undiluted was studied against the antigen (DDT) coated onto the immuno module. The homogenous response of the anti DDT anti serum was adequate to identify DDT. Fifteen samples each of milk, buttermilk, cheese, kalakhand and khoa were quantitatively analyzed for DDT using ELISA as well as GC methods. DDT was present only below detectable limit (BDL) (5 ng g -1 ) in milk and milk products obtained from organized dairies in and around Chennai city, India. The percentage recovery was above 70% ensuring that the ELISA method developed for DDT is dependable. The developed ELISA method can be helpful in detecting the residual amount of DDT present in milk and milk products. The analysis of pesticides in samples by ELISA and GC was comparable.

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“…The major advantages with deltamethrin (DLM) are their photostability, high potency, and low toxicity to birds and mammals [8,9]. Deltamethrin is registered for use on various crops, including cotton, corn, cereals, soybeans, and vegetables, for pests such as mites, ants, weevils, and beetles, and the control of parasites on animals [10]. Presently it is also used in the control of malaria and other vector borne diseases [2,11].…”

Section: Introductionmentioning

confidence: 99%

Determination of Deltamethrin in Mice Plasma and Immune Organs by Simple Reversed-Phase HPLC

Kumar¹,

Dubey²,

Kant³

et al. 2016

Acta Chromatographica

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Deltamethrin, a well-known type 2 synthetic pyrethroid insecticide, is a widespread environmental toxicant. It has potential to accumulate in body fluids and tissues due to its lipophilic characteristics. The immune system is among the most sensitive targets regarding toxicity of environmental pollutants. Various methods are available in the literature to analyze deltamethrin (DLM) concentration in plasma and tissues, but regarding the immune organs, only one gas chromatography–tandem mass spectrometry (GC–MS/MS) method (on spleen tissues) has been reported. In the present investigation, a rapid and sensitive high-performance liquid chromatography (HPLC) method has been developed and validated to determine DLM concentration in plasma, thymus, and spleen using zaleplone as an internal standard. Liquid chromatography (LC) separation is performed on an Agilent Zorbax® C8 column (250 mm × 4.6 mm, i.d., 5 μm) with isocratic elution using a mobile phase consisting of acetonitrile–5 mM KH2PO4 (70:30, v/v) at a flow rate of 1 mL min−1. The lower limit of quantification (LLOQ) for DLM is 10 ng mL−1 (plasma, thymus, and spleen). The method has been validated in terms of establishing linearity, specificity, sensitivity, recovery, accuracy, and precision (intra- and inter-day) and stabilities study. This validated method was successfully applied to a pharmacokinetic and tissue distribution study of DLM in mice.

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Selective extraction of pyrethroid pesticide residues from milk by solid-matrix dispersion

Cited by 62 publications

References 9 publications

Simultaneous determination of pyrethroids from pesticide residues in porcine muscle and pasteurized milk using GC

Simultaneous determination of pyrethroids from pesticide residues in porcine muscle and pasteurized milk using GC

Development of ELISA kit for the assay of ichlorodiphenyltrichloroethane in milk and milk products

Determination of Deltamethrin in Mice Plasma and Immune Organs by Simple Reversed-Phase HPLC

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