Selective fluorescence swing from cysteine to glutathione by switchover from solid to in situ probe in 100% water and bio-imaging studies for living species

Das, Sanju; Sarkar, Yeasmin; Mukherjee, Santanu; Bandyopadhyay, Jaya; Samanta, Subhodip; Parui, Partha Pratim; Ray, Ambarish

doi:10.1016/j.snb.2014.11.145

Cited by 25 publications

(5 citation statements)

References 48 publications

(91 reference statements)

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“…The serum samples were diluted to make sure the GSH concentration in the samples was in a predefined linear range and to obtain quantitative recovery of the GSH. As shown in Table , the results obtained using Eu(DPA) 3 @Lap-Tris-Cu 2+ demonstrated the reliability of this approach for determination of GSH in practical samples …”

Section: Resultsmentioning

confidence: 54%

“…As shown in Table 1, the results obtained using Eu(DPA) 3 @Lap-Tris-Cu 2+ demonstrated the reliability of this approach for determination of GSH in practical samples. 40 Interaction Mechanism between Eu(DPA) 3 @Lap-Tris, Cu 2+ , and GSH. It is known that transition metal ions, particularly Cu 2+ ions, are able to quench Ln 3+ luminescence, and this behavior has been primarily observed in copper-based complexes containing Ln 3+ .…”

Section: ■ Introductionmentioning

confidence: 99%

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Luminescent Lanthanide-Based Organic/Inorganic Hybrid Materials for Discrimination of Glutathione in Solution and within Hydrogels

Chen

Wang

Chai

et al. 2017

ACS Appl. Mater. Interfaces

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Glutathione (GSH) as a biothiol is an essential peptide related to various diseases. Although multiple strategies for biothiols detection have been developed, there is increasing demand for sensors that can differentiate GSH from cysteine (Cys) and homocysteine (Hcy), owing to the similar structures and thiol groups in these amino acids. Herein, we report a novel Eu/LAPONITE (Lap)-based organic/inorganic hybrid material for selective detection of GSH via an "off-on" process. The fluorescence of Eu(DPA)@Lap-Tris can be quenched by Cu through photoinduced electron transfer (PET). The addition of GSH into the Eu(DPA)@Lap-Tris/Cu system induces the removal of Cu from Eu(DPA)@Lap-Tris and blocks PET, resulting in the recovery of fluorescence. This proposed assay demonstrates higher selectivity toward GSH than Cys and Hcy, and showed a detection limit of 162 nM within a linear range of 0.5-30 μM. Unlike other GSH selective sensors, this platform could be formed into a hydrogel while its sensitivity was maintained. The sensitive response to GSH in serum samples makes this platform an efficient tool for biological applications because of its ease of preparation, high selectivity, good biocompatibility, and low toxicity.

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Section: Resultsmentioning

confidence: 54%

Section: ■ Introductionmentioning

confidence: 99%

Luminescent Lanthanide-Based Organic/Inorganic Hybrid Materials for Discrimination of Glutathione in Solution and within Hydrogels

Chen

Wang

Chai

et al. 2017

ACS Appl. Mater. Interfaces

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“…Compared with traditional detection methods, fluorescence (FL) detection methods have the advantages of high sensitivity, noninvasive performance, and high-resolution imaging. − To date, there have been numerous reports on fluorescent probes designed for detecting Cys. − However, these reported Cys probes suffer from the disadvantage of short analytical wavelengths, thus limiting their application in vivo. Near-infrared (NIR) fluorescent probes have long excitation/emission wavelengths, enabling deep tissue penetration, reducing background fluorescence of proteins and minimizing photodamage of biological samples, which are very beneficial for biological applications. − Several fluorescent probes for detecting Cys in the NIR region have been developed (Table S1).…”

Section: Introductionmentioning

confidence: 99%

Tumor-Targeting Probe for Dual-Modal Imaging of Cysteine In Vivo

Yang

Chao

et al. 2023

Anal. Chem.

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Cysteine (Cys) is a crucial biological thiol that has a vital function in preserving redox homeostasis in organisms. Studies have shown that Cys is closely related to the development of cancer. Thus, it is necessary to design an efficient method to detect Cys for an effective cancer diagnosis. In this work, a novel tumor-targeting probe (Bio-Cy-S) for dualmodal (NIR fluorescence and photoacoustic) Cys detection is designed. The probe exhibits high selectivity and sensitivity toward Cys. After reaction with Cys, both NIR fluorescence and photoacoustic signals are activated. Bio-Cy-S has been applied for the dual-modal detection of Cys levels in living cells, and it can be used to distinguish normal cells from cancer cells by different Cys levels. In addition, the probe is capable of facilitating dual-modal imaging for monitoring changes in Cys levels in tumor-bearing mice. More importantly, the excellent tumor-targeting ability of the probe greatly improves the signal-to-noise ratio of imaging. To the best of our knowledge, this is the first Cys probe to combine targeting and dual-modal imaging performance for cancer diagnosis.

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“…If its content is too high, this will lead to neurotoxicity [ 10 ] and cardiovascular disease [ 11 ] ; if its content is too low, this will lead to decreased haematopoietic function, oedema [ 12 ] , and slow growth. [ 13–15 ] High levels of Hcy have been linked to osteoporosis, ischaemic stroke, and Alzheimer's disease. [ 16 ] Abnormal levels of GSH may reduce cellular resistance to oxidative damage, causing liver damage, and cancer.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and application of benzoxazole derivative‐based fluorescent probes for naked eye recognition

et al. 2020

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A fluorescent probe to detect biothiols (GSH/Hcy/Cys) was synthesized with benzothiazole as the fluorophore and nitromethane as the recognition group. The recognition mechanism of the fluorescent probe was deduced. It was found that a nitroalkene in the molecule was used as the recognition site for this probe and reacted with the mercapto group of the biothiols by electrophilic addition, significantly enhancing the fluorescent signal. Experimental results showed that the fluorescent probe had a low detection limit, good selectivity, strong anti-interference ability, and good naked eye recognition. The probe could detect biothiols (GSH/Hcy/Cys) in 20% organic solvent, with detection limits of 0.33 μM, 0.70 μM,

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Selective fluorescence swing from cysteine to glutathione by switchover from solid to in situ probe in 100% water and bio-imaging studies for living species

Cited by 25 publications

References 48 publications

Luminescent Lanthanide-Based Organic/Inorganic Hybrid Materials for Discrimination of Glutathione in Solution and within Hydrogels

Luminescent Lanthanide-Based Organic/Inorganic Hybrid Materials for Discrimination of Glutathione in Solution and within Hydrogels

Tumor-Targeting Probe for Dual-Modal Imaging of Cysteine In Vivo

Synthesis and application of benzoxazole derivative‐based fluorescent probes for naked eye recognition

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