Selective Functionalization of Microstructured Surfaces by Laser‐Assisted Particle Transfer

Abstract

Microcavity arrays represent millions of different reaction compartments to screen for e.g. molecular interactions, exogenous factors for cells or enzymatic activity. We present a novel method to selectively synthesize different compounds in arrays of microcavities with up to 1,000,000 cavities per cm 2 . In our approach, polymer microparticles with embedded preactivated monomers are selectively transferred into microcavities with laser radiation. After particle patterning, heating of the particle matrix simultaneously leads to diffusion and coupling of the monomers inside each microcavity separately. This method exhibits flexibility, 2 not only in the choice of compounds, but also in the choice of particle matrix material, which determines the chemical reaction environment. The laser-assisted selective functionalization of microcavities can be easily combined with the intensively growing number of laser applications for patterning of molecules and cells, which is useful for the development of novel biological assays.

Spatial Modes of Laser-Induced Mass Transfer in Micro-Gaps

“…The lower surface (acceptor slide) was a glass slide functionalized with a polymer layer (10:90 amino-terminated poly(ethylene glycol)methacrylate and methyl methacrylate) bearing free amino groups (PEPperPRINT GmbH, Heidelberg, Germany). Acceptor slides with dry-etched micro-cavities were also used in Reference [8].…”

Enhancing the Microparticle Deposition Stability and Homogeneity on Planer for Synthesis of Self-Assembly Monolayer

“…The result of this study will be applied to create homogeneity in bioparticle [ 11 ] deposition in the future. The deposited bioparticles can be addressed in different methods and used for monomer synthesis, such as electrical fields from microelectrodes [ 19 , 25 ] or laser [ 34 , 35 , 36 , 37 ], and can be used for several applications, such as antibody analysis [ 38 ] or surface functionalization [ 39 ]. The microparticles were dehumidified in the desiccator with a molecular sieve overnight before the experiment.…”

Combinatorial Particle Patterning

“…First, the particles are deposited into microcavities, then, the particles are sintered. Finally, they are transferred by laser radiation from their microcavities into the cavities of a synthesis slide ( Figure a) . Microstructured substrates can be homogeneous filled with particles in a simple wiping step.…”

“…Red signals correspond to stained glycine; green signals correspond to stained biotin. Reproduced with permission . Copyright 2016, John Wiley and Sons.…”