Selective Growth Inhibitory Effect of Biochanin A Against Intestinal Tract Colonizing Bacteria

Sklenickova, Olga; Flesar, Jaroslav; Kokoška, Ladislav; Vlková, Eva; Halamová, Kateřina; Jan, Malik

doi:10.3390/molecules15031270

Cited by 43 publications

(34 citation statements)

References 53 publications

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“…These results indicate that BCA has selective antimicrobial activity and could have directly altered the cellulolytic guild. The results of this study are consistent with previous studies that have demonstrated selective antimicrobial activity of BCA against both fungi (Kr€ amer et al 1984;Weidenb€ orner et al 1990;Rojas et al 2006) and bacteria (Gnanamanickam and Smith 1980;Dastidar et al 2004;Sklenickova et al 2010). The mechanism of the direct antimicrobial activity of BCA is unknown and requires future research to elucidate.…”

Section: Discussionsupporting

confidence: 90%

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Biochanin A improves fibre fermentation by cellulolytic bacteria

2017

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Aims: The objective was to determine the effect of the isoflavone biochanin A (BCA) on rumen cellulolytic bacteria and consequent fermentative activity. Methods and Results: When bovine microbial rumen cell suspensions (n = 3) were incubated (24 h, 39°C) with ground hay, cellulolytic bacteria proliferated, short-chain fatty acids were produced and pH declined. BCA (30 lg ml À1 ) had no effect on the number of cellulolytic bacteria or pH, but increased acetate, propionate and total SCFA production. Addition of BCA improved total digestibility when cell suspensions (n = 3) were incubated (48 h, 39°C) with ground hay, Avicel, or filter paper. Fibrobacter succinogenes S85, Ruminococcus flavefaciens 8 and Ruminococcus albus 8 were directly inhibited by BCA. Synergistic antimicrobial activity was observed with BCA and heat killed cultures of cellulolytic bacteria, but the effects were species dependent. Conclusions: These results indicate that BCA improves fibre degradation by influencing cellulolytic bacteria competition and guild composition. Significance and Impact of the Study: BCA could serve as a feed additive to improve cellulosis when cattle are consuming high-fibre diets. Future research is needed to evaluate the effect of BCA on fibre degradation and utilization in vivo.

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Section: Discussionsupporting

confidence: 90%

“…; Sklenickova et al . ). The mechanism of the direct antimicrobial activity of BCA is unknown and requires future research to elucidate.…”

Section: Discussionmentioning

confidence: 97%

Biochanin A improves fibre fermentation by cellulolytic bacteria

2017

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“…In contrast to previously described antibacterial effect of compound 9 against some strains of Clostridia (Sklenickova et al . ), Chlamydia (Pohjala et al . ), Mycobacterium (Lechner et al .…”

Section: Resultsmentioning

confidence: 99%

The relationship between structure and in vitro antibacterial activity of selected isoflavones and their metabolites with special focus on antistaphylococcal effect of demethyltexasin

Hummelova

Rondevaldova

Balastikova

et al. 2014

Lett Appl Microbiol

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The results of the structure-activity relationship (SAR) analysis identified ortho-dihydroxyisoflavones as a class of antibacterially effective compounds emphasizing the hydroxyl groups at C-5, 6 and 7 positions as crucial supposition for the antibacterial action of plant isoflavones and their metabolites. Demethyltexasin, an isoflavones' metabolite present in the human body through enterohepatic recycling of soya bean isoflavones (daidzein, genistein), showed the most potent antibacterial activity, especially against various strains of Staphylococcus aureus (including MDR and MRSA). The significance of this study is a deepening of the knowledge on isoflavones' SAR and identification of the antistaphylococcal activity of demethyltexasin, which suggest that metabolites of isoflavones can be even more potent antibacterial agents than their precursors.

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“…Lastly, an implication of our study is that future efforts aimed at reducing false positives in IDEXX assays could constructively focus on reducing the growth of nontarget bacteria, e.g., by testing a variety of selective growth-inhibiting compounds available for members of the Lactobacillales and Clostridiales and for Vibrio spp. (18,32,38,50,52).…”

Section: Discussionmentioning

confidence: 99%

Cultivation-Independent Analysis of Bacteria in IDEXX Quanti-Tray/2000 Fecal Indicator Assays

Sercu

Werfhorst

Murray

et al. 2011

Appl Environ Microbiol

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Monitoring microbiological water quality is important for protecting water resources and the health of swimmers. Routine monitoring relies on cultivating fecal indicator bacteria (FIB), frequently using defined substrate technology. Defined substrate technology is designed to specifically enrich for FIB, but a complete understanding of the assay microbiology requires culture-independent analysis of the enrichments. This study aimed to identify bacteria in positive wells of Colilert and Enterolert Quanti-Tray/2000 (IDEXX Laboratories) FIB assays in environmental water samples and to quantify the degree of false-positive results for samples from an urban creek by molecular methods. Pooled Escherichia coli-and Enterococcus-positive Quanti-Tray/2000 enrichments, either from urban creek dry weather flow or municipal sewage, harbored diverse bacterial populations based on 16S rRNA gene sequences and terminal restriction fragment length polymorphism analyses. Target taxa (coliforms or enterococci) and nontarget taxa (Vibrio spp., Shewanella spp., Bacteroidetes, and Clostridium spp.) were identified in pooled and individual positive Colilert and Enterolert wells based on terminal restriction fragments that were in common with those generated in silico from clone sequences. False-positive rates of between 4 and 23% occurred for the urban creek samples, based on the absence of target terminal restriction fragments in individual positive wells. This study suggests that increased selective inhibition of nontarget bacteria could improve the accuracy of the Colilert and Enterolert assays.Quantifying fecal pollution in recreational waters is important for protecting the health of swimmers. Current standards for microbiological water quality in the United States and elsewhere are based on culturable fecal indicator bacteria (FIB), i.e., total coliforms, fecal coliforms, or Escherichia coli, and enterococci (21,53,57). Various methods are used to quantify FIB, including multiple-tube fermentation, membrane filtration, and defined substrate technologies (9,16,45). All rely on temperature, substrate, and selective growth inhibitors to select for FIB (23,29). The commercially available defined substrate technologies Colilert and Enterolert (IDEXX Laboratories, Westbrook, ME) are accepted by the U.S. Environmental Protection Agency as alternatives to the multiple-tube fermentation and membrane filtration methods for fresh, marine, and estuarine surface waters (54). Specific enzyme-substrate relationships are the basis of both assays (45). In the Colilert assay, total coliforms and E. coli are indicated by a yellow or a yellow and a fluorescent metabolite, respectively. Positive Enterolert assays are indicated by a fluorescent metabolite. The manufacturer-supplied tray format is more convenient than multiple-tube fermentation and membrane filter techniques (20, 45). However, there is evidence that the Colilert and Enterolert assays are not specific (2,6,17,43,51).False-positive Colilert or Enterolert readings could occur when there i...

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Selective Growth Inhibitory Effect of Biochanin A Against Intestinal Tract Colonizing Bacteria

Cited by 43 publications

References 53 publications

Biochanin A improves fibre fermentation by cellulolytic bacteria

Biochanin A improves fibre fermentation by cellulolytic bacteria

The relationship between structure and in vitro antibacterial activity of selected isoflavones and their metabolites with special focus on antistaphylococcal effect of demethyltexasin

Cultivation-Independent Analysis of Bacteria in IDEXX Quanti-Tray/2000 Fecal Indicator Assays

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