Selective Immobilization of Protein Clusters on Polymeric Nanocraters

Valsesia, Andrea; Colpo, Pascal; Meziani, T.; Bretagnol, F.; Lejeune, M.; Rossi, François; Bouma, Anita; García-Parajo, María F.

doi:10.1002/adfm.200500653

Cited by 46 publications

(46 citation statements)

References 16 publications

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“…It can be seen that limit of detection is shifted toward lower concentration, with best results obtained at smaller nanopattern dimensions (200 nm nanostructure). Similar enhancement has been observed with different techniques (SPRi, QCM, ELISA test) [67,68,73,74]. The level of amplification depends on the size of the nanostructure and increases as the size of the patterns decreases.…”

Section: Protein Adsorption On Nanopatternssupporting

confidence: 60%

“…The nanostructures produced by colloidal and electron-beam lithography have been extensively studied with different biological tests and protein adsorption experiments [17,67,68,69,70,73]. We present below two significant examples indicating the particular behaviour of nanostructures as compared to non-patterned surfaces.…”

Section: Protein Adsorption On Nanopatternsmentioning

confidence: 99%

“…Structures of 200 nm up to 1000 nm and more can be obtained with this process, with coverage typically of 90% on a few mm 2 . Details of the experiments are given in [67,68]. The shape, aspect ratio of the nanostructures can be controlled as well by tuning the plasma parameters [56].…”

Section: Surface Patterning Using Colloidal Lithographymentioning

confidence: 99%

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Applications and challenges of plasma processes in nanobiotechnology

Rossi¹,

Colpo²

2011

J. Phys. D: Appl. Phys.

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Abstract:We present an overview of the possibilities offered by plasma technologies, in particular the combination plasma polymers deposition, colloidal lithography, ebeam lithography and microcontact printing, to produce micro and nanostructured surfaces with chemical and topographical contrast for applications in NanoBiotechnology. It is shown that chemical and topographical patterns can be obtained on different substrates, with dimensions down to few tenths of 10 nm. The applications of these nanostructured surfaces in biology, biochemistry and biodetection are presented and the advantages and limitation of the plasma techniques in this context underlined.

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Section: Protein Adsorption On Nanopatternssupporting

confidence: 60%

Section: Protein Adsorption On Nanopatternsmentioning

confidence: 99%

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Applications and challenges of plasma processes in nanobiotechnology

Rossi¹,

Colpo²

2011

J. Phys. D: Appl. Phys.

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“…Cold plasmas have been used as a step in nanotopographies / nanopatterns manufacturing, [15,[43][44][45][46] especially in reactive ion etching of predeposited or self-assembled patterned masks into a surface. [1][2][27][28][29][30][47][48] Recent reports investigate the direct use of plasma for nanopatterning of siliconebased and nanocomposite materials, [49] for producing self-assembled layers of special polymers on surfaces, [47] and for directly producing micro-and nano-topography on PP, PET, PMMA, PTFE surfaces.…”

Section: Introductionmentioning

confidence: 99%

Nanotopography Engineering on Homopolymeric Surfaces Using Cold Plasma Generated Charges

Manolache

Gutierrez

Hall

2010

Macromolecular Symposia

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Summary: Cold plasma as source for charged particles was used to induce nanotopographies on homopolymeric surfaces (nylon 12, PMMA, PMA, etc.). Hydrogen and helium plasmas were successfully used for surface nanoengineering of polymers using inductively coupled or atmospheric pressure non-equilibrium barrier discharge reactors. AFM analyses reveal the presence of nanotopographies on the treated surfaces. Physical factors control the process below the T g and chemical factors dominate the process above T g . Pyrolysis GC/MS analyses have been performed in order to obtain more information about the plasma processes. Cold plasma chemical processes, including charges effects are discussed as tools that open-up new ways for nanoengineering of the polymers' surfaces with specific functionalities and / or topography. Future nanomanufacturing techniques can generate anti-scratch, superhydrophobic or superhydrophilic properties on surface of every day use polymeric products by simple and convenient plasma enhanced processes.

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“…The DEGDME solution was also allowed to react for 60 min before being rinsed by 50 µl PBS twice and dried with nitrogen. For deposition of dry DEGDME by PECVD, the open PMMA flow-cell was subjected to plasma cleaning and activation in an argon plus oxygen mixed plasma, with a flow rate of 100 standard cubic centimetre per minute (sccm) of argon and oxygen, for 1 min at 100 W and then the DEGDME deposition was carried out by sequential deposition of tetraethylorthosilicate (TEOS) and diethylene glycol dimethyl ether (DEGDME) by low pressure Plasma Enhanced Chemical Vapor Deposition (PECVD) [31][32][33]. The flow of TEOS and DEGDME were controlled using needle valve and the deposition was carried out in an argon plasma.…”

Section: Deposition Of Blocking Agents Into Pmma Microfluidic Flow-cellsmentioning

confidence: 99%

Evaluation of Different Nonspecific Binding Blocking Agents Deposited Inside Poly(methyl methacrylate) Microfluidic Flow-Cells

Gubala

Gandhiraman

et al. 2011

Langmuir

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Poly(methyl methacrylate) (PMMA) flow-cells containing microwells were deposited with different nonspecific binding blocking agents namely bovine serum albumin (BSA), cationic lipid (DOTAP) and diethylene glycol dimethyl ether (DEGDME). Water contact angle (WCA) and atomic force microscope (AFM) measurements were carried out to confirm the successful depositions of BSA, DOTAP, DEGDME onto the PMMA surfaces. Fluorescent intensity measurements were performed to evaluate the degree of non-specific adsorption of Cy5-labeled anti-IgG proteins onto plain and oxygen plasma treated (PT) PMMA flow-cells as well as PMMA flow-cells deposited with different above-mentioned blocking agents. We then employed a label-free detection method called total internal reflection ellipsometry (TIRE) to evaluate the stability of the deposited blocking agents inside the PMMA flow-cells. It was found that while DOTAP was the best agent for blocking the non-specific adsorption, it could be removed from the PMMA surfaces of the flow-cells upon rinsing with phosphate buffer saline (PBS) and later deposited back onto the Au-coated glass sensing substrate of the TIRE. The removal of the blocking agents from PMMA surfaces and their deposition onto the sensing substrate were further manifested by measuring the kinetics and the amount of adsorbed anti-a-hCG proteins. Overall, the dry DEGDME coating by plasma enhanced chemical vapor deposition (PECVD) showed very good blocking and excellent stability for subsequent assay inside the microwells. Our results could be useful when one considers what blocking agents should be used for PMMA-based microfluidic immunosensor or biosensor devices by looking at both the blocking efficiency and the stability of the blocking agent.

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Selective Immobilization of Protein Clusters on Polymeric Nanocraters

Cited by 46 publications

References 16 publications

Applications and challenges of plasma processes in nanobiotechnology

Applications and challenges of plasma processes in nanobiotechnology

Nanotopography Engineering on Homopolymeric Surfaces Using Cold Plasma Generated Charges

Evaluation of Different Nonspecific Binding Blocking Agents Deposited Inside Poly(methyl methacrylate) Microfluidic Flow-Cells

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