Selective in vitro degradation of the sialylated fraction of germ-free rat mucins by the caecal flora of the rat

Fontaine, Nicole; Meslin, J. C.; Doré, Joël

doi:10.1051/rnd:19980309

Cited by 14 publications

(7 citation statements)

References 19 publications

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“…Although the physiological role of distinct mucin subtypes is not well understood, the state of "health" of the gut seems to be related to the degree of mucin maturation with sulfated acidic mucin considered as more mature and so more protective for the gut (Fontaine et al, 1998). The present results might imply no change in the capacity of mucus to protect the gut against bacterial infection.…”

Section: Resultsmentioning

confidence: 60%

Dietary fibre and indigestible protein increase ileal glycoprotein output without impacting colonic crypt goblet cells in weaned piglets

et al. 2007

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Section: Resultsmentioning

confidence: 60%

Dietary fibre and indigestible protein increase ileal glycoprotein output without impacting colonic crypt goblet cells in weaned piglets

et al. 2007

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“…In the small bowel of Klf4 mutant mice, there is a reduction in staining for acidic mucin (as demonstrated by the Alcian blue stain) but no change in staining for neutral mucin (as demonstrated by the PAS stain) and the goblet cell-specific marker, Muc2. As acidic mucin is considered the more mature form of mucin produced by goblet cells (Fontaine et al, 1998), these findings suggest that Klf4 is required for maturation of goblet cells in the small intestine. In the colon, Klf4 is required not only for the maturation but differentiation of goblet cells as demonstrated by the significant reduction in the number and size of goblet cells, as well as in staining for Muc2, and both acidic and neural mucins in the colon of Klf4 ΔIS mice compared to controls (Fig.…”

Section: Discussionmentioning

confidence: 94%

Altered intestinal epithelial homeostasis in mice with intestine-specific deletion of the Krüppel-like factor 4 gene

Ghaleb

McConnell

Kaestner

et al. 2011

Developmental Biology

117

111

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The zinc finger transcription factor, Krüppel-like factor 4 (KLF4), is expressed in the post-mitotic, differentiated epithelial cells lining the intestinal tract and exhibits a tumor suppressive effect on intestinal tumorigenesis. Here we report a role for KLF4 in maintaining homeostasis of intestinal epithelial cells. Mice with conditional ablation of the Klf4 gene from the intestinal epithelium were viable. However, both the rates of proliferation and migration of epithelial cells were increased in the small intestine of mutant mice. In addition, the brush-border alkaline phosphatase was reduced as was expression of ephrine-B1 in the small intestine, resulting in mispositioning of Paneth cells. In the colon of mutant mice, there was a reduction of the differentiation marker, carbonic anhydrase-1, and failure of differentiation of goblet cells. Mechanistically, deletion of Klf4 from the intestine resulted in a general activation of genes in the Wnt pathway and a global reduction in expression of genes encoding regulators of differentiation. Taken together, these data provide new insights into the function of KLF4 in regulating postnatal proliferation, migration, differentiation, and positioning of intestinal epithelial cells and demonstrate an essential role for KLF4 in maintaining normal intestinal epithelial homeostasis in vivo.

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“…In one study mucins from germ-free rats were incubated with total cecal microbiota from conventionally raised rats. Sialylated mucins were degraded more rapidly than the neutral or sulfated forms suggesting an overall optimized use of sialic acids by intestinal bacteria [72]. Although ocular fluid from many humans is sterile, some studies have shown that other people maintain a commensal bacterial population without incident.…”

Section: The Salad Bar-substrate Availability At Mucosal Surfacesmentioning

confidence: 99%

Unified Theory of Bacterial Sialometabolism: How and Why Bacteria Metabolize Host Sialic Acids

Vimr

2013

ISRN Microbiology

145

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Sialic acids are structurally diverse nine-carbon ketosugars found mostly in humans and other animals as the terminal units on carbohydrate chains linked to proteins or lipids. The sialic acids function in cell-cell and cell-molecule interactions necessary for organismic development and homeostasis. They not only pose a barrier to microorganisms inhabiting or invading an animal mucosal surface, but also present a source of potential carbon, nitrogen, and cell wall metabolites necessary for bacterial colonization, persistence, growth, and, occasionally, disease. The explosion of microbial genomic sequencing projects reveals remarkable diversity in bacterial sialic acid metabolic potential. How bacteria exploit host sialic acids includes a surprisingly complex array of metabolic and regulatory capabilities that is just now entering a mature research stage. This paper attempts to describe the variety of bacterial sialometabolic systems by focusing on recent advances at the molecular and host-microbe-interaction levels. The hope is that this focus will provide a framework for further research that holds promise for better understanding of the metabolic interplay between bacterial growth and the host environment. An ability to modify or block this interplay has already yielded important new insights into potentially new therapeutic approaches for modifying or blocking bacterial colonization or infection.

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Selective in vitro degradation of the sialylated fraction of germ-free rat mucins by the caecal flora of the rat

Cited by 14 publications

References 19 publications

Dietary fibre and indigestible protein increase ileal glycoprotein output without impacting colonic crypt goblet cells in weaned piglets

Dietary fibre and indigestible protein increase ileal glycoprotein output without impacting colonic crypt goblet cells in weaned piglets

Altered intestinal epithelial homeostasis in mice with intestine-specific deletion of the Krüppel-like factor 4 gene

Unified Theory of Bacterial Sialometabolism: How and Why Bacteria Metabolize Host Sialic Acids

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