Selective in Vivo and in Vitro Sampling of Proteins Using Miniature Ultrafiltration Sampling Probes

Schneiderheinze, Jeffrey M.; Hogan, Barry L.

doi:10.1021/ac960309u

Cited by 30 publications

(14 citation statements)

References 20 publications

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“…The characteristics of the semipermeable membrane used will affect the size of molecules that are allowed in the ultrafiltrate sample. For small molecules, the recovery is (as for microdialysis) close to 100% (261,272), whereas the observed in vitro recovery for albumin has been 74 -100% depending on dialysis time (415), indicating that ultrafiltration can be used for sampling of macromolecules. To better allow for sampling of interstitial proteins, membranes with a molecular mass cutoff of 400 kDa have been used, and tissue fluid has been collected from ear (206) and abdominal skin (205) in mice using this approach, yielding protein concentrations of 100 and 8.6 g/ml, respectively.…”

Section: Capillary Ultrafiltrationmentioning

confidence: 97%

Interstitial Fluid and Lymph Formation and Transport: Physiological Regulation and Roles in Inflammation and Cancer

Wiig

Swartz²

2012

Physiological Reviews

575

574

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The interstitium describes the fluid, proteins, solutes, and the extracellular matrix (ECM) that comprise the cellular microenvironment in tissues. Its alterations are fundamental to changes in cell function in inflammation, pathogenesis, and cancer. Interstitial fluid (IF) is created by transcapillary filtration and cleared by lymphatic vessels. Herein we discuss the biophysical, biomechanical, and functional implications of IF in normal and pathological tissue states from both fluid balance and cell function perspectives. We also discuss analysis methods to access IF, which enables quantification of the cellular microenvironment; such methods have demonstrated, for example, that there can be dramatic gradients from tissue to plasma during inflammation and that tumor IF is hypoxic and acidic compared with subcutaneous IF and plasma. Accumulated recent data show that IF and its convection through the interstitium and delivery to the lymph nodes have many and diverse biological effects, including in ECM reorganization, cell migration, and capillary morphogenesis as well as in immunity and peripheral tolerance. This review integrates the biophysical, biomechanical, and biological aspects of interstitial and lymph fluid and its transport in tissue physiology, pathophysiology, and immune regulation.

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Section: Capillary Ultrafiltrationmentioning

confidence: 97%

Interstitial Fluid and Lymph Formation and Transport: Physiological Regulation and Roles in Inflammation and Cancer

Wiig

Swartz²

2012

Physiological Reviews

575

574

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“…Since slow diffusion will affect recovery values, time-consuming recovery calculations are required with this technique [35]. Ultrafiltration sampling has been used to detect various molecules, such as calcium, potassium, sodium and glucose [36], but detection and identification of proteins/peptides in these samples was generally ignored until Schneiderheinze and Hogan sampled proteins in vivo and in vitro with miniature ultrafiltration sampling probes [37]. In our laboratory, we integrated an ultrafiltration technique with MS for in vivo sampling and identification of secreted proteins/peptides in tissue fluids [38][39][40][41].…”

Section: Sampling Tissue Fluids Challenges In the Sampling Of Tissue mentioning

confidence: 99%

Recent advances in protein profiling of tissues and tissue fluids

Yang¹,

Huang²

2007

Expert Review of Proteomics

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Creating protein profiles of tissues and tissue fluids, which contain secreted proteins and peptides released from various cells, is critical for biomarker discovery as well as drug and vaccine target selection. It is extremely difficult to obtain pure samples from tissues or tissue fluids, however, and identification of complex protein mixtures is still a challenge for mass spectrometry analysis. Here, we summarize recent advances in techniques for extracting proteins from tissues for mass spectrometry profiling and imaging. We also introduce a novel technique using a capillary ultrafiltration (CUF) probe to enable in vivo collection of proteins from the tissue microenvironment. The CUF probe technique is compared with existing sampling techniques, including perfusion, saline wash, fine-needle aspiration and microdialysis. In this review, we also highlight quantitative mass spectrometric proteomic approaches with, and without, stable-isotope labels. Advances in quantitative proteomics will significantly improve protein profiling of tissue and tissue fluid samples collected by CUF probes.

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“…The membranes used as filters would be capable of differentiating between complexes which are different by an order of magnitude. Alternatively, other recent developments in the form of capillary electrophoresis, micro-dialysis, and miniaturized ultra-filtration can be used [RJ91,SH96,Bak96].…”

Section: Extended Model Operationsmentioning

confidence: 99%

Micro flow bio-molecular computation

Gehani

Reif

1999

Biosystems

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In this paper we provide a model for micro-flow based bio-molecular computation (MF-BMC). It provides an abstraction for the design of algorithms which account for the constraints of the model. Our MF-BMC model uses abstractions of both the recombinant DNA (RDNA) technology as well as of the micro-flow technology and takes into account both of their limitations. For example, when considering the efficiency of the recombinant DNA operation of annealing, we take into account the limitation imposed by the concentration of the reactants. The fabrication technology used to construct MEMS is limited to constructing relatively thin 3D structures. We abstract this by limiting the model to a small constant number of layers (as is done with VLSI models).Besides our contribution of the MF-BMC model, the paper contains two other classes of results. The main result is the volume and time efficient algorithm for message routing in the MF-BMC model, specifically useful for PA-Match [Rei98a]. We will show that routing of strands between chambers will occur in time O( N ·D m·n ), where N is the number of strands in the MF-BMC, n is the number of chambers where RDNA operations are occurring, D is the diameter of the topology of the layout of the chambers, and m is proportional to the channel width. Operations that need annealing, such as PA-Match, are shown feasible in O( N 2 log N n n ) volume instead of the previous use of Ω(N 2 ) volume, with reasonable time constraints. Applications of the volume efficient algorithm include the use of the Join operation for databases, logarithmic depth solutions to SAT (Boolean formula satisfiability) problems and parallel algorithms that execute on a PRAM. Existent algorithms can be mapped to ones that work efficiently in the MF-BMC model, whereas previous methods for applications such as PRAM simulation in BMC were not both time and volume efficient. Our other class of results are theoretical lower bounds on the quantities of DNA and the time needed to solve a problem in the MF-BMC model, analogous to lower bounds in VLSI. We bound the product BT from below, and further show that BT 2 has a stronger lower bound of I 2 . Here B is the maximum amount of information encoded in the MF-BMC system at a time, T is the time for an algorithm to complete, and I is the information content of a problem.

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Selective in Vivo and in Vitro Sampling of Proteins Using Miniature Ultrafiltration Sampling Probes

Cited by 30 publications

References 20 publications

Interstitial Fluid and Lymph Formation and Transport: Physiological Regulation and Roles in Inflammation and Cancer

Interstitial Fluid and Lymph Formation and Transport: Physiological Regulation and Roles in Inflammation and Cancer

Recent advances in protein profiling of tissues and tissue fluids

Micro flow bio-molecular computation

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