Selective induction of an immune response in human external secretions by ingestion of bacterial antigen.

Městecký, Jiří; McGhee, Jerry R.; Arnold, Roland R.; Michalek, S M; Prince, Shirley J.; Babb, James L.

doi:10.1172/jci108986

Cited by 272 publications

(133 citation statements)

References 35 publications

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“…Oral ingestion of nonreplicating substances induces the concomitant appearance of IgA antibodies in external secretions (3). However, large quantities of antigen are required to induce secretory antibody responses by the mucosal routes, and the responses are generally modest and of short duration (4).…”

Section: Introductionmentioning

confidence: 99%

Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.

Quiding

Nordström²,

Kilander³

et al. 1991

J. Clin. Invest.

211

123

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We have examined secretory antibody and cell-mediated immune responses to oral cholera vaccine in the human gastrointestinal mucosa. Freshly isolated peripheral blood lymphocytes and intestinal lymphocytes obtained by enzymatic dispersion of duodenal biopsies were assayed for numbers of total and vaccine specific immunoglobulin-secreting cells by enzyme-linked immunospot assay (ELISPOT) techniques; the frequency of cells secreting interferon-y (IFN--y) was also examined by a new modification of the ELISPOT technique.After booster immunizations with oral cholera vaccine, large numbers of cholera toxin-specific antibody-secreting cells (ASC) appeared in the small intestine. The responses were dominated by IgA ASC. A single immunization, performed 5 mo after the initial vaccinations, gave rise to an ASC response similar to that seen after the first booster immunization, with respect to both magnitude and isotype distribution. Each of the immunizations also evoked an ASC response in blood which was of lower magnitude than that seen in the small intestine, and comprised similar proportions of IgA and IgG ASC. A booster immunization also resulted in increased frequencies of IFN-y-secreting cells, but this increase was confined to the duodenal mucosa.This study establishes the feasibility of studying, at the single-cell level, intestinal immune reactivity in humans. Furthermore, it indicates that the small intestinal mucosa is an enriched source of IFN-7. It also demonstrates marked differences between intestinal and peripheral blood immune responses after enteric immunization, and confirms the notion that the mucosal immune system in humans displays immunological memory. (J. Clin. Invest. 1991. 88:143-148.)

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Section: Introductionmentioning

confidence: 99%

Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.

Quiding

Nordström²,

Kilander³

et al. 1991

J. Clin. Invest.

211

123

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“…The development of the concept of the common mucosal immune system, whereby the local presentation of an antigen at one mucosal surface can stimulate an immune response at distant mucosal surfaces (6,8,9,11,16,17), has led to the examination of oral vaccination as a means of stimulating an effective immune response in the respiratory tract. The live orally administered typhoid vaccine organism, Salmonella typhi Ty2la, has been considered as a potential carrier of heterologous protective antigens (3,5,13), so the demonstration that S. typhi Ty2la is capable of stimulating a respiratory tract immune response following oral administration would permit the subsequent examination of this or similar attenuated organisms as vectors for antigens of respiratory pathogens.…”

mentioning

confidence: 99%

Specific immune response in the human respiratory tract following oral immunization with live typhoid vaccine

Forrest

LaBrooy

Robinson

et al. 1990

Advances in Mucosal Immunology

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Specific antibody responses in the lower respiratory tract of human subjects to orally administered Salmonella typhi Ty2la are reported. These responses, predominantly of the immunoglobulin G class, were determined to be a transudate from serum. These results were supported by the similarity in responses to parenteral administration of heat-killed typhoid vaccine. Specific immunoglobulin A antibody was a poor contributor to the respiratory antibody response to either vaccine.

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“…Although ingestion of microbial antigens is known to induce the concomitant appearance of S-IgA antibodies in human secretions (11)(12)(13), the existence of circulating antigen-sensitized IgA precursors has not been shown in human subjects. In the present study, we demonstrate the transient occurrence of such cells in the peripheral blood of orally immunized volunteers.…”

mentioning

confidence: 99%

IgA antibody-producing cells in peripheral blood after antigen ingestion: evidence for a common mucosal immune system in humans.

Czerkinsky¹,

Prince²,

Michalek³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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233

115

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The finding that ingestion of antigens results in the selective induction of IgA antibodies in external secretions suggests that antigen sensitizes Peyer's patch lymphoid cells, which migrate to mucosal sites and generate local secretory IgA (S-IgA) antibody responses. Evidence for a common mucosal immune system in humans has been scanty because of the difficulty in demonstrating migratory behavior of Peyer's patch cells. In the present study, peripheral blood mononuclear cells (PBMC) from human volunteers who had ingested capsules containing killed Streptococcus mutans were assayed for spontaneous antibody-producing cells. Four of five volunteers exhibited circulating IgA-producing cells within 7 days and reached maximum responses by days 10-12. One

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Selective induction of an immune response in human external secretions by ingestion of bacterial antigen.

Cited by 272 publications

References 35 publications

Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.

Intestinal immune responses in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-gamma production and evokes local immunological memory.

Specific immune response in the human respiratory tract following oral immunization with live typhoid vaccine

IgA antibody-producing cells in peripheral blood after antigen ingestion: evidence for a common mucosal immune system in humans.

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