Selective Induction of Interleukin-12 in Reconstructed Human Epidermis by Chemical Allergens

Corsini, Emanuela; Limiroli, Elena; Marinovich, Marina; Cohen, C.; Roguet, R.; Galli, Corrado L.

doi:10.1177/026119299902700205

Cited by 8 publications

(4 citation statements)

References 15 publications

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“…For determination of IL-10 mRNA levels, semiquantitative RT-PCR was performed as previously described. 18 Briefly, 4 · 10 6 alveolar macrophages were lysed in 1 ml of Triazol TM (Invitrogen, Carlsbad, CA). Total RNA was extracted following the supplier's instructions.…”

Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Increased carrageenan‐induced acute lung inflammation in old rats

Corsini¹,

Paola²,

Viviani³

et al. 2005

Immunology

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Summary Ageing is associated with increased susceptibility to lung infections and delayed resolution of pulmonary infiltrates. The purpose of this study was to investigate the effect of age on the onset of carrageenan‐induced lung inflammation. When compared with carrageenan‐treated young rats (3 months old), old rats (> 18 months old) exhibited a preponderance of pleural exudation and polymorphonuclear cell infiltration. Lung myeloperoxidase activity, an index of neutrophil infiltration and activation, was significantly increased in old rats in comparison with young rats. Consistent with the biochemical markers of inflammation, increased lung damage, as assessed by nitrosative stress and lipid peroxidation, was observed in carrageenan‐treated old rats. In the lung exudate obtained from old rats, a significant reduction in interleukin‐10 (IL‐10) was observed, while similar expression of monocyte chemotactic protein‐1 was induced, suggesting that a decrease in IL‐10 rather than increased chemotaxis may account for the preponderance of the inflammatory cellular infiltrate in old rats. Similar to the in vivo situation, freshly isolated alveolar macrophages obtained from old rats produced less IL‐10. This defective IL‐10 production could be explained by a reduction in the cAMP‐dependent signalling pathway, which mediates IL‐10 production. Indeed, we found decreased cAMP‐responsive element binding protein (CREB) and phosphorous‐CREB (P‐CREB) expression in old rats, which may account for reduced IL‐10 production in old rats.

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Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Increased carrageenan‐induced acute lung inflammation in old rats

Corsini¹,

Paola²,

Viviani³

et al. 2005

Immunology

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“…RT-PCR For determination of ADRP mRNA levels, semiquantitative RT-PCR was performed as previously described (Corsini et al, 1999). Brie£y, con£uent cells cultured in six-well plates were treated with increasing concentrations of SDS (0^30 mg per ml) in 1.4 ml of medium supplemented with 1% FBS for 18 h. Monolayers were washed once with PBS and cells were lyzed in 1 ml of Triazol (Invitrogen, Carlsbad, CA).…”

Section: Cells and Experimental Proceduresmentioning

confidence: 99%

Induction of Adipose Differentiation Related Protein and Neutral Lipid Droplet Accumulation in Keratinocytes by Skin Irritants

Corsini

Viviani

Zancanella

et al. 2003

Journal of Investigative Dermatology

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Keratinocytes play an important role in skin irritation. In an attempt to investigate mechanistic bases of human skin irritation response, we recently identified the upregulation by skin irritants of adipose differentiation related protein (ADRP) in reconstituted human epidermis. ADRP is a lipid-storage-droplet-associated protein, governing deposition and release of lipids from droplets. The purpose of this study was to characterize, in a human keratinocyte cell line (NCTC 2544), sodium-dodecyl-sulfate-induced ADRP expression, to identify the biochemical events that lead to ADRP expression, and to understand its function in sodium dodecyl sulfate cytotoxicity. Sodium dodecyl sulfate induced a concentration- and time-related production of ADRP that was associated with lipid droplet accumulation. Lipid accumulation following sodium dodecyl sulfate treatment was due to intracellular redistribution rather than lipid neosynthesis, as indicated by equivalent 14C-oleate and 14C-acetate incorporations. Other skin irritants, namely benzalkonium chloride, tributyltin, and 12-O-tetradecanoylphorbol 13-acetate, also induce lipid droplet accumulation. Sodium-dodecyl-sulfate-induced ADRP expression and lipid droplet accumulation were modulated by the calcium chelator BAPTA, indicating a role of calcium in ADRP induction. Decrease of sodium-dodecyl-sulfate-induced ADRP expression by specific ADRP antisense oligonucleotide resulted in increased cytotoxicity, indicating a protective role of ADRP and lipid accumulation in the process of cell damage induced by skin irritants. ADRP expression was also induced in vivo following treatment with sodium dodecyl sulfate in an experimental model of skin irritation, indicating that the in vitro model represents irritation.

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“…It was also likely that animal-derived trypsin would have been used in a number of other methods, for example, for subculturing the cells used to prepare conditioned medium, 96 or in the manufacturing of commercially available reconstructed human epidermis. 128,129…”

Section: Discussionmentioning

confidence: 99%

The Overt and Hidden Use of Animal-Derived Products in Alternative Methods for Skin Sensitisation: A Systematic Review

et al. 2019

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In vitro methods that can replace animal testing in the identification of skin sensitisers are now a reality. However, as cell culture and related techniques usually rely on animal-derived products, these methods may be failing to address the complete replacement of animals in safety assessment. The objective of this study was to identify the animal-derived products that are used as part of in vitro methods for skin sensitisation testing. Thus, a systematic review of 156 articles featuring 83 different in vitro methods was carried out and, from this review, the use of several animal-derived products from different species was identified, with the use of fetal bovine serum being cited in most of the methods (78%). The use of sera from other animals, monoclonal antibodies and animal proteins were also variously mentioned. While non-animal alternatives are available and methods free of animal-derived products are emerging, most of the current methods reported used at least one animal-derived product, which raises ethical and technical concerns. Therefore, to deliver technically and ethically better in vitro methods for the safety assessment of chemicals, more effort should be made to replace products of animal origin in existing methods and to avoid their use in the development of new method protocols.

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Selective Induction of Interleukin-12 in Reconstructed Human Epidermis by Chemical Allergens

Cited by 8 publications

References 15 publications

Increased carrageenan‐induced acute lung inflammation in old rats

Increased carrageenan‐induced acute lung inflammation in old rats

Induction of Adipose Differentiation Related Protein and Neutral Lipid Droplet Accumulation in Keratinocytes by Skin Irritants

The Overt and Hidden Use of Animal-Derived Products in Alternative Methods for Skin Sensitisation: A Systematic Review

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