Selective inhibition of matrix metalloproteinase 2 (gelatinase A) by barium chloride

Ziouti, N.; Mastronikolis, N.S.; Ap, Andonopoulos; Georgakopoulos, Constantinos D.; Vynios, Demitrios H.

doi:10.1016/j.ab.2005.12.014

Cited by 2 publications

(1 citation statement)

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“…1 9 10 4 cells containing 2% fetal bovine serum with various final concentrations of NH 4 Al(SO 4 ) 2 were incubated in 96-plates well for 2 days Inorganic salt MMP inhibitors had rarely been reported: de Souza et al analyzed MMP-2 and MMP-9 in the conditioned media of gingival explants by gelatin zymography and showed inhibition of MMP-2 and MMP-9 by ZnSO 4 with half inhibition concentration of 15 and 40 lM, respectively whereas CuSO 4 , HgSO 4 and SnCl 2 showed less efficient inhibition potential (de Souza et al 2000). In another case, Ziouti et al (2006) showed 5 mM barium chloride selectively inhibited MMP-2 but not MMP-9 by zymography, while other bivalent metal salts such as Fe, Ni, Co, Mn, Cd, Hg, and Zr, showed not inhibitory effect on the activity of MMP-2, and SrCl 2 slightly suppressed MMP-2 activity.…”

Section: Discussionmentioning

confidence: 97%

Aluminum ammonium sulfate dodecahydrate purified from traditional Chinese medicinal herb Korean monkshood root is a potent matrix metalloproteinase inhibitor

et al. 2012

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Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases and key regulators for many physiological and pathological functions. The MMP inhibitors have been shown to modulate diseases such as cancer, inflammation, and cardiovascular diseases. In this paper we tracked the MMP inhibitory activities of the traditional Chinese medicinal herb Korean Monkshood Root. The purified active ingredient was identified by the elemental analysis, infrared spectrum (IR) and X-ray diffraction as aluminum ammonium sulfate dodecahydrate. This inorganic compound showed inhibitory activities toward a number of MMP family members. In particular, it has a strong inhibitory effect toward MMP-2 and MMP-9, with IC50 values of 0.54 and 0.50 μM, respectively. Further analysis suggested that the MMP inhibitory activity is mainly due to Al(3+). Cell viability assays using human fibrosarcoma HT1080 cells showed aluminum ammonium sulfate had minimal cyto-toxicity with a concentration up to 500 μM. However, within 50 μM, it exhibited significant inhibition of cell invasion. To our knowledge, there has been no previous report of inorganic form of the MMP inhibitor with strong inhibitory activity. Our results for the first time showed that aluminum ammonium sulfate is an inorganic form of MMP inhibitor with high potency, and can be used to interfere with MMP related cellular processes.

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Section: Discussionmentioning

confidence: 97%