Selective inhibition of the germination of Bacillus megaterium spores by alkyl- p-hydroxybenzoates.

Hydroxypropyl methylcellulose-beeswax edible coatings formulated with antifungal food additives to reduce alternaria black spot and maintain postharvest quality of cold-stored cherry tomatoes

“…Parabens are in the undissociated form at pH values of most foods (pKa = 8.5) and are effective over a wide pH range of 4-8 (Thompson, 1994). Paraben salts like SMP and SEP are more soluble in water than their correspondent parabens and they might interfere on both the germinative and vegetative phases of microbial development, although it has been reported that fungal spore germination is much more susceptible than vegetative growth (Watanabe and Takesue, 1976). It has been suggested that the general mode of action of these salts is through an uncoupling of oxidative phosphorylation, inhibition of NAD + and FAD-linked mitochondrial respiration, or the reduction of mitochondrial membrane potential (Soni et al, 2001).…”

Bacterial spores and chemical sporicidal agents

“…These include alcohols, aldehydes, phenols and cresols, parabens, sorbic acid, and mercuric chloride (2,3,47,66,68,100,128,148,149,156,166,168,171,172,181,190,193,195,198,219,220,223,234; B. M. Lund, Ph.D. dissertation, University of London, London, England, 1962). This inhibition (Table 10) occurs at concentrations that are closely related to those that inhibit the growth of vegetative bacteria.…”

“…This is apparent from the results of studies with phenols (128,148,149,181), formaldehyde (219), alcohols (220), and parabens (157,234). These findings suggest a fairly loose binding of these agents to a site(s) on the spore surface since mere washing is often sufficient to dislodge the inhibitor.…”

Aproximaciones a una estrategia integrada para el control no contaminante de las podredumbres verde y azul en poscosecha de cítricos

“…23 SPP might interfere on both the germinative and vegetative phases of microbial development, but spore germination is much more susceptible to SPP than fungal vegetative growth. 24 Paraben salts like SPP are more soluble in water than their correspondent parabens and they act as phenolic weak acids in aqueous solutions. When a weak acid is dissolved in water, equilibrium is established between undissociated acid molecules and charged ions, the proportion of undissociated acid increasing with lower pH.…”

“…Los ensayos de influencia temporal en la actividad preventiva del tratamiento con PSi a 90 mM, revelaron un control satisfactorio en la incidencia y severidad de la podredumbre verde, sólo cuando el lapso de tiempo entre el tratamiento y la inoculación fue de 2 h, con respecto al resto de los tratamientos (24,48, 72 y 96 h), los cuales no causaron ningún efecto en la efectividad de PSi a 90 mM. Estos resultados sugieren que residuos de ingrediente activo de PSi podían encontrarse en cantidades suficientes en el interior de la herida para afectar la presencia de conidios y el crecimiento de P. digitatum, mientras que para el resto de los tiempos esta cantidad de residuos de PSi habría disminuido considerablemente pudiendo haberse perdido por difusión.…”