Selective Interaction of Colistin with Lipid Model Membranes

Dupuy, Fernando Gabriel; Pagano, Isabella; Andenoro, Kathryn; Peralta, Marcela; Elhady, Yasmene; Heinrich, Frank; Tristram-Nagle, Stephanie

doi:10.1016/j.bpj.2017.11.2496

Cited by 2 publications

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“… 1 Pure colistin (sulfate) is not available as a reference standard and it is a mixture of more than 30 polypeptide antibiotics that are mainly composed of colistin A and colistin B 2 . The chemical structure of colistin (see Figure 1 ) is a linear trilipopeptide linked to a cyclic heptapeptide 3 . The fatty acid tail consists of eight carbons for colistin A and seven for colistin B.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of colistin A/B and colistin methanesulfonate in biological samples using hydrophilic interaction chromatography tandem mass spectrometry

Gijsen

Brantegem

et al. 2020

Drug Testing and Analysis

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Colistin (polymyxin E) is a polycation antibiotic which is increasingly used (administered as colistin methanesulfonate, CMS) as a salvage therapy in critically ill patients with multidrug resistant Gram‐negative infections. Even though colistin has been used for more than 50 years, its metabolic fate is poorly understood. One of the current challenges for studying the pharmacokinetics (PK) is the precise and accurate determination of colistin in in vitro and in vivo studies. In the present study, we developed and validated a series of sensitive and robust liquid chromatography tandem mass spectrometry (LC–MS/MS) methods for analysing biological samples obtained from in vitro and in vivo disposition assays. After a zinc acetate‐mediated precipitation, hydrophilic–lipophilic‐balanced solid phase extraction (HLB‐SPE) was used for the extraction of colistin. The compounds were retained on a hydrophilic interaction liquid chromatography (HILIC) column and were detected by MS/MS. CMS was quantified by determining the produced amount of colistin during acidic hydrolysis. The developed methods are sensitive with lower limits of quantification varying between 0.009 μg/mL and 0.071 μg/mL for colistin A, and 0.002 μg/mL to 0.013 μg/mL for colistin B. The intra‐ and inter‐day precision and accuracy were within ±15%. Calibration curves of colistin were linear (0.063 μg/mL to 8.00 μg/mL) within clinically relevant concentration ranges. Zinc acetate‐mediated precipitation and the use of a HILIC column were found to be essential. The developed methods are sensitive, accurate, precise, highly efficient and allow monitoring colistin and CMS in biological samples without the need for an internal standard.

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Section: Introductionmentioning

confidence: 99%

Quantitative determination of colistin A/B and colistin methanesulfonate in biological samples using hydrophilic interaction chromatography tandem mass spectrometry

Gijsen

Brantegem

et al. 2020

Drug Testing and Analysis

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The Combating Impact of Biogenic Silver Nano Particles and Antibiotics on Combating Multidrug-Resistant Pseudomonas

Almutairi,

Alshammeri,

aldakhili

2023

JCPP

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This study aimed to assess the synergistic effect of silver nanoparticles (AgNPs) in combination with different antibiotics against multidrug-resistant Pseudomonas aeruginosa (MDR P. aeruginosa) infections. A total of 120 surgical or burn wound samples were collected from a tertiary care hospital. The samples were cultured on cetrimide agar plates and then heated to 370 degrees Celsius. The isolates were identified based on colony shape, Gram's staining, and various biochemical tests. The antibiotic susceptibility profile was determined using the Kirby-Bauer disc diffusion technique according to CLSI 2022 recommendations. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. The antibacterial activity of AgNPs was evaluated using the agar well diffusion technique and the microdilution method. The synergistic effect of antibiotics and AgNPs was assessed using the Checkerboard method. Out of the 120 samples, 46 (38.8%) were confirmed as positive for P. aeruginosa, and among them, 33 were identified as MDR P. aeruginosa. Seven representative isolates were selected for further analysis. The antibacterial activity of AgNPs showed a maximum zone of inhibition of 12mm at 4mg/ml and a minimum of 2.5mm at 1mg/ml using the agar well diffusion method. The MIC and MBC of AgNPs indicated that all isolates were inhibited at a concentration of 250mg/ml. The Checkerboard results revealed that colistin and gentamicin exhibited complete synergism with AgNPs, while ciprofloxacin showed partial synergism with AgNPs.

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Selective Interaction of Colistin with Lipid Model Membranes

Cited by 2 publications

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Quantitative determination of colistin A/B and colistin methanesulfonate in biological samples using hydrophilic interaction chromatography tandem mass spectrometry

Quantitative determination of colistin A/B and colistin methanesulfonate in biological samples using hydrophilic interaction chromatography tandem mass spectrometry

The Combating Impact of Biogenic Silver Nano Particles and Antibiotics on Combating Multidrug-Resistant Pseudomonas

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