Selective isolation and noninvasive analysis of circulating cancer stem cells through Raman imaging

Cho, Hyeon-Yeol; Hossain, Md. Khaled; Lee, Jinho; Han, Jiyou; Lee, Hun Joo; Kim, Kyeong-Jun; Kim, Jong-Hoon; Lee, Ki‐Bum; Choi, Jeong‐Woo

doi:10.1016/j.bios.2017.11.049

Cited by 58 publications

(52 citation statements)

References 70 publications

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“…Raman spectrum by SERS analysis of the MDA-MB-231 and MCF-7 exhibited different shapes and peaks so the classification of the cells could be relatively easy with the nondestructive method by measurement of Raman spectrum. Noninvasive analysis of the circulating cancer stem cells (CCSCs) was successfully conducted with microfluidic chips and Raman reporter-labelled Au nanoparticles [116] (Figure 4b). Solution-based analyte with several cells was flown into the cell and micropillars separated circulating cells and other small biomolecules.…”

Section: Sers-based Analytical Platform With Metallic Nanostructures mentioning

confidence: 99%

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Metallic Nanoparticle-Based Optical Cell Chip for Nondestructive Monitoring of Intra/Extracellular Signals

et al. 2020

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The biosensing platform is noteworthy for high sensitivity and precise detection of target analytes, which are related to the status of cells or specific diseases. The modification of the transducers with metallic nanoparticles (MNPs) has attracted attention owing to excellent features such as improved sensitivity and selectivity. Moreover, the incorporation of MNPs into biosensing systems may increase the speed and the capability of the biosensors. In this review, we introduce the current progress of the developed cell-based biosensors, cell chip, based on the unique physiochemical features of MNPs. Mainly, we focus on optical intra/extracellular biosensing methods, including fluorescence, localized surface plasmon resonance (LSPR), and surface-enhanced Raman spectroscopy (SERS) based on the coupling of MNPs. We believe that the topics discussed here are useful and able to provide a guideline in the development of new MNP-based cell chip platforms for pharmaceutical applications such as drug screening and toxicological tests in the near future.

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Section: Sers-based Analytical Platform With Metallic Nanostructures mentioning

confidence: 99%

“…(b) Raman-active nanoprobe-based chip platform with the capability of simultaneous detection, isolation, and further analysis of circulating cancer stem cells (CCSCs) and various circulating tumor cells (CTCs) subtypes though Raman imaging. Reproduced with permission[116]. Copyright 2018, ACS Publications.…”

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Metallic Nanoparticle-Based Optical Cell Chip for Nondestructive Monitoring of Intra/Extracellular Signals

et al. 2020

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“…This Raman barcoding system provides the potential to overcome a “multiplexing ceiling” from existing optical materials. Cho and his coworkers developed a Raman barcoding-based biosensor with GNPs to diagnose and analyze circulating cancer stem cells (CCSCs) in the presence of circulating tumor cells (CTCs) ( Figure 2 C) [ 42 ]. For this approach, five different combinations of Raman-active nanoprobes (RANs) were prepared, each consisting of a GNP (as a Raman signal enhancer), aromatic thiol (as a Raman reporter), antibodies, and thiolated DNA (as a capturing linker).…”

Section: Gold Nanoparticle-based Surface-enhanced Raman Spectroscomentioning

confidence: 99%

“…( A ) Highly ordered octahedral gold nanoparticle (GNP) arrays for Raman signal enhancement; ( B ) pH-dependent SERS signal differences between serotonin and tryptophan based on gold nanoparticles; ( C ) Raman barcoding-based cancer subtyping platform based on combination of Raman reporter tagged gold nanoparticles; ( D ) detection of glucose in live mouse tissue based on dimerization of gold nanoparticles. ( A ) Figure reproduced with permission from [ 37 ], © 2011 Elsevier; ( B ) Figure reproduced with permission from [ 38 ], © 2010 Society of Photo-Optical Instrumentation Engineers; ( C ) Figure reproduced with permission from [ 42 ], © 2018 Elsevier; ( D ) Figure reproduced with permission from [ 43 ], © 2017 American Chemical Society.…”

Section: Figurementioning

confidence: 99%

Application of Gold Nanoparticle to Plasmonic Biosensors

Lee

Cho

Choi

et al. 2018

IJMS

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Gold nanoparticles (GNPs) have been widely utilized to develop various biosensors for molecular diagnosis, as they can be easily functionalized and exhibit unique optical properties explained by plasmonic effects. These unique optical properties of GNPs allow the expression of an intense color under light that can be tuned by altering their size, shape, composition, and coupling with other plasmonic nanoparticles. Additionally, they can also enhance other optical signals, such as fluorescence and Raman scattering, making them suitable for biosensor development. In this review, we provide a detailed discussion of the currently developed biosensors based on the aforementioned unique optical features of GNPs. Mainly, we focus on four different plasmonic biosensing methods, including localized surface plasmon resonance (LSPR), surface-enhanced Raman spectroscopy (SERS), fluorescence enhancement, and quenching caused by plasmon and colorimetry changes based on the coupling of GNPs. We believe that the topics discussed here are useful and able to provide a guideline in the development of novel GNP-based biosensors in the future.

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“…Furthermore, the combination of the great intensity of the signals obtained with this technique with the latest developments in the fields of microfluidics and optical spectroscopy (2D charge-coupled devices (CCDs)), enables real time (i.e., online) monitoring of continuous flows. In fact, online SERS detection has been already demonstrated using microfluidic devices for a number of analytes including pesticides and metals in water, explosives and pollutants, drugs, cancer cells and metabolites, and many others [ 26 , 29 , 30 , 31 , 32 , 33 ]. Therefore, the concurrent rise of SERS and microfluidic technologies in recent years is more of an expected result than coincidence.…”

Section: Introductionmentioning

confidence: 99%

Surface-Enhanced Raman Scattering Spectroscopy and Microfluidics: Towards Ultrasensitive Label-Free Sensing

Kant

Abalde‐Cela

2018

Biosensors

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Raman scattering and surface-enhanced Raman scattering (SERS) spectroscopy have demonstrated their potential as ultrasensitive detection techniques in the past decades. Specifically, and as a result of the flourishing of nanotechnology, SERS is nowadays one of the most powerful sensing techniques, not only because of the low detection limits that it can achieve, but also for the structural information that it offers and its capability of multiplexing. Similarly, microfluidics technology is having an increased presence not only in fundamental research, but also in the industry. The latter is because of the intrinsic characteristics of microfluidics, being automation, high-throughput, and miniaturization. However, despite miniaturization being an advantage, it comes together with the need to use ultrasensitive techniques for the interrogation of events happening in extremely small volumes. The combination of SERS with microfluidics can overcome bottlenecks present in both technologies. As a consequence, the integration of Raman and SERS in microfluidics is being investigated for the label-free biosensing of relevant research challenges.

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Selective isolation and noninvasive analysis of circulating cancer stem cells through Raman imaging

Cited by 58 publications

References 70 publications

Metallic Nanoparticle-Based Optical Cell Chip for Nondestructive Monitoring of Intra/Extracellular Signals

Metallic Nanoparticle-Based Optical Cell Chip for Nondestructive Monitoring of Intra/Extracellular Signals

Application of Gold Nanoparticle to Plasmonic Biosensors

Surface-Enhanced Raman Scattering Spectroscopy and Microfluidics: Towards Ultrasensitive Label-Free Sensing

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