Selective killing of human cancer cells by polyunsaturated fatty acids

Bégin, Michel E.; Das, Undurti N.; Ells, G.; Horrobin, David F.

doi:10.1016/0262-1746(85)90084-8

Cited by 171 publications

(69 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As proposed previously by Begin et al (1988) andCanuto et al (1991), this suggests that an oxidative process is involved in the effects of PUFAs on malignant cell lines rather than a cyclo-oxygenase-generated product. The inhibition of growth of the pancreatic cancer cell lines observed in the present study occurred at lower concentrations of PUFA than others have described with different, non-pancreatic, malignant cell lines (Dippenaar et al, 1982;Fugiwara et al, 1983;Begin et al, 1985Begin et al, , 1986Begin et al, , 1988Canuto et al, 1991), indicating that pancreatic cancer cells may be particularly sensitive to the effects of PUFAs, at least in vitro.…”

Section: Effect Of Medium Replenishmentsupporting

confidence: 44%

“…The concentration at which PUFAs can cause a loss of cell viability and inhibit cell growth is known to vary depending on the cell density (Begin et al, 1985(Begin et al, , 1986. However, the concentration at which EPA had an inhibitory effect on cell growth was higher in the experiments performed using cells grown in 25 cm2 flasks than when the cells were grown in 96 well microplates, despite the seeding concentrations being approximately the same (5 x IO0 to 1 x IO0 cells ml-').…”

Section: Effect Of Medium Replenishmentmentioning

confidence: 99%

“…A number of studies have suggested that certain polyunsaturated fatty acids (PUFAs) can inhibit the growth of a variety of human cancer cell lines in vitro (Wica et al, 1979;Dippenaar et al, 1982;Fugiwara et al, 1983;Begin et al, 1985Begin et al, , 1986. Moreover, the effects of PUFAs have been shown to be selective for cancer cells without affecting normal cells in vitro (Begin et al, 1986).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Effect of eicosapentaenoic acid and other fatty acids on the growth in vitro of human pancreatic cancer cell lines

Falconer¹,

Ross²,

Fearon³

et al. 1994

Br J Cancer

132

View full text Add to dashboard Cite

Summary A number of polyunsaturated fatty acids have been shown to inhibit the growth of malignant cells in vitro. To investigate whether fatty acids modify the growth of human pancreatic cancer, lauric, stearic, palmitic, oleic, linoleic, alpha-linolenic, gamma-linolenic, arachidonic, docosahexaenoic and eicosapentaenoic (EPA) acids were each incubated with the cells lines MIA PaCa-2, PANC-1 and CFPAC at concentrations ranging from 1.25 JAM to 5011M and the effect of each fatty acid on cell growth was examined. All the polyunsaturated fatty acids tested had an inhibitory effect, with EPA being the most potent (ID50 2.5 -5 gM).Monounsaturated or saturated fatty acids were not inhibitory. The action of EPA could be reversed with the anti-oxidant vitamin E acetate or with oleic acid. The cyclo-oxygenase inhibitors indomethacin and piroxicam had no effect on the action of EPA. The action of EPA appeared to be associated with the generation of lipid peroxides, although the level of lipid peroxidation did not always appear to correlate directly with the extent of cell death. The ability of certain fatty acids to inhibit significantly the growth of three human pancreatic cancer cell lines in vitro at concentrations which could be achieved in vivo suggests that administration of such fatty acids may be of therapeutic benefit in patients with pancreatic cancer.

show abstract

Section: Effect Of Medium Replenishmentsupporting

confidence: 44%

Section: Effect Of Medium Replenishmentmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of eicosapentaenoic acid and other fatty acids on the growth in vitro of human pancreatic cancer cell lines

Falconer¹,

Ross²,

Fearon³

et al. 1994

Br J Cancer

132

View full text Add to dashboard Cite

show abstract

“…LA and ALA were also effective, but at much higher concentrations compared with other PUFAs (Bégin et al, 1985;1986a;1986b;Das, 1991). In general, it is believed that n-6 fatty acids are unhelpful in cancer prevention and therapy, whereas n-3 fatty acids are beneficial.…”

Section: Introductionmentioning

confidence: 99%

“…Polyunsaturated fatty acids (PUFAs), such as GLA, DGLA, AA, EPA, and DHA, can bring about the action of EFAs, and hence are also called functional EFAs. Our previous studies showed that, under some specific conditions, PUFAs can induce tumor cell death, though the sensitivity of various cancer cell lines to different fatty acids was found to be variable, depending on the type of cancer cells tested and the type and concentration of the fatty acids used (Bégin et al, 1985;1986a;1986b;Das, 1991).…”

Section: Introductionmentioning

confidence: 99%

Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes

et al. 2010

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Polyunsaturated fatty acids (PUFAs) possess anti-cancer action both in vitro and in vivo. In the present study, we detected cell viability with methyl thiazolyl tetrazolium (MTT) assay and cell membrane permeability with propidium iodide (PI) fluorescence dyeing, and calculated cell membrane fluidity change as fluorescence anisotropy. Fatty acid content in cells was measured by gas chromatography/mass spectroscopy (GC/MS), and the relationship between fatty acid composition and cell viability was studied. We observed that n-6 PUFA linoleic acid (LA) inhibited tumor cell growth at high concentrations (≥300 µmol/L), while low concentrations (100-200 µmol/L) seemed to promote cell proliferation. Analyses of cell membrane permeability, cell membrane fluidity, and cell fatty acid composition suggested that the anti-cancer action of LA could be related to changes in the ratio of n-6 to n-3 PUFAs. We observed that pre-incubation of cancer cells with 100 µmol/L LA for 24 h enhanced cell sensitivity to the cytotoxic action of LA, whereas undifferentiated cell line LoVo seemed to have a distinct path in LA-induced death. These results showed that one of the mechanisms by which supplementation of LA induces cancer cell death could be altering the ratio of n-6/n-3 PUFAs, and this may be related to cell differentiation status.

show abstract

Application of a continuous bioreactor cascade to study the effect of linoleic acid on hybridoma cell physiology

Kisztelinski

Alink

Rietjens

et al. 2006

Biotechnol. Bioeng.

View full text Add to dashboard Cite

The aim of the present study is to demonstrate the use of controlled bioreactors for toxicological studies. As a model system the effect of linoleic acid on hybridoma cells is studied in two well-controlled continuously operated bioreactors placed in series. In the first reactor the effect on rapid proliferating cells can be studied, while in the second reactor a special steady state is created, which allows studying the effect on apoptotic cells. Experiments are done at 0, 25, and 50 microM linoleic acid. At the end of the experiment with 50 microM linoleic acid, the concentration of linoleic acid is increased stepwise to determine the cytotoxic level. For rapid proliferating cells exposed to 25 and 50 microM stimulation of growth was observed. At 50 microM there was at the same time an increase in cell death through apoptosis. For stressed apoptotic cells linoleic acid caused partial growth inhibition at 25 and 50 microM and arrest of cell proliferation in the G(2)/M phase at 50 microM. For both, rapid proliferating cells and stressed apoptotic cells, complete growth inhibition occurred at 85 microM, with cells being arrested in the G(2)/M phase and dying mainly through necrosis. Cells in the bioreactor system appeared to be more sensitive towards linoleic acid than cells grown in multi-well plates. (IC(50) = 300 microM; IC(100) = 400 microM). Altogether the results of the present study reveal that the biostat experiments allow detailed analysis of the effect of a bioactive ingredient on cell physiology and behavior.

show abstract

Selective killing of human cancer cells by polyunsaturated fatty acids

Cited by 171 publications

References 7 publications

Effect of eicosapentaenoic acid and other fatty acids on the growth in vitro of human pancreatic cancer cell lines

Effect of eicosapentaenoic acid and other fatty acids on the growth in vitro of human pancreatic cancer cell lines

Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes

Application of a continuous bioreactor cascade to study the effect of linoleic acid on hybridoma cell physiology

Contact Info

Product

Resources

About