Selective Migration of α-Smooth Muscle Actin-Positive Myofibroblasts toward Fibronectin in the Boyden's Blindwell Chamber

Kawamoto, Masashi; Matsunami, Takakuni; Ertl, Ronald F.; Fukuda, Yuh; Ogawa, Maki; Spurzem, John R.; Yamanaka, Nobuaki; Rennard, Stephen I.

doi:10.1042/cs0930355

Cited by 25 publications

(12 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experiments investigating the effects of α-SMA expression on fibroblast migration have produced conflicting results. Lung fibroblasts expressing large amounts of α-SMA exhibited increased migratory activity towards fibronectin [53]. However, other studies demonstrated that fibroblast strains with increased α-SMA expression had reduced migratory capacity and that α-SMA knockdown increased cell motility [54].…”

Section: Discussionmentioning

confidence: 99%

The role of α-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling

Shinde

Humeres

Frangogiannis

2017

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

405

257

View full text Add to dashboard Cite

Cardiac myofibroblasts play an important role in myocardial remodeling. Although α-smooth muscle actin (α-SMA) expression is the hallmark of mature myofibroblasts, its role in regulating fibroblast function remains poorly understood. We explore the effects of the matrix environment in modulating cardiac fibroblast phenotype, and we investigate the role of α-SMA in fibroblast function using loss- and gain-of-function approaches. In murine myocardial infarction, infiltration of the infarct border zone with abundant α-SMA-positive myofibroblasts was associated with scar contraction. Isolated cardiac fibroblasts cultured in plates showed high α-SMA expression localized in stress fibers, exhibited activation of focal adhesion kinase (FAK), and synthesized large amounts of extracellular matrix proteins. In contrast, when these cells were cultured in collagen lattices, they exhibited marked reduction of α-SMA expression, negligible FAK activation, attenuated collagen synthesis, and increased transcription of genes associated with matrix metabolism. Transforming Growth Factor-βl-mediated contraction of fibroblast-populated collagen pads was associated with accentuated α-SMA synthesis. In contrast, serum- and basic Fibroblast Growth Factor-induced collagen pad contraction was associated with reduced α-SMA expression. α-SMA siRNA knockdown attenuated contraction of collagen pads populated with serum-stimulated cells. Surprisingly, α-SMA overexpression also reduced collagen pad contraction, suggesting that α-SMA is not sufficient to promote contraction of the matrix. Reduced contraction by α-SMA-overexpressing cells was associated with attenuated proliferative activity, in the absence of any effects on apoptosis. α-SMA may be implicated in contraction and remodeling of the extracellular matrix, but is not sufficient to induce contraction. α-SMA expression may modulate cellular functions, beyond its effects on contractility.

show abstract

Section: Discussionmentioning

confidence: 99%

The role of α-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling

Shinde

Humeres

Frangogiannis

2017

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

405

257

View full text Add to dashboard Cite

show abstract

“…Fibroblasts from human ACL that migrated into collagen-glycosaminoglycan matrix express α-SM actin [15]. also, fibroblasts that migrated across Boyden chambers were more likely to express α-SM actin than nonmigrated cells [16]. Furthermore, downregulation of α-SM actin by steroid was associated with inhibition of tendon cell migration [17].…”

Section: Introductionmentioning

confidence: 95%

Therapeutic Ultrasound Stimulation of Tendon Cell Migration

Tsai

Chen

Pang

et al. 2008

Connective Tissue Research

View full text Add to dashboard Cite

Ultrasound is a therapeutic agent commonly used to treat sports-related tendinopathy. Tendon healing requires tendon cells migration to the repair site, followed by the proliferation and synthesis of extracellular matrix. This study was designed to determine the effect of ultrasound on migration of tendon cells intrinsic to rat Achilles tendon. Furthermore, the existence of a correlation between this effect and the expression of the contractile actin isoform, alpha-smooth muscle (SM) actin, which is associated with cell mobility, was also examined. Cell migration was evaluated by transwell filter migration assay. The mRNA expressions of alpha-SM actin were determined by reverse transcription-polymerase chain reaction. Dose-dependent ultrasound enhancement of tendon cells migration through the transwell filter was demonstrated. Using immunofluorescence stain for alpha-SM actin, the percentages of alpha-SM actin-positive cells of total cells, nonmigrated cells, and migrated cells on the filter were calculated. Ultrasound-treated cells which had migrated to the bottom side of the filter were more likely to express alpha-SM actin than migrated control cells and nonmigrated cells. However, there was no change of mRNA and protein expression of alpha-SM actin as well as expression of FAK and p-FAK. In conclusion, ultrasound stimulates tendon cell migration in association with increased expression of alpha-SM actin of tendon cells.

show abstract

“…The decrease in vimentin expression might therefore explain the decrease in migration capacity found in PC3‐educated MSCs. αSMA expression in fibroblasts has also been shown to correlate with increased migration and so the decrease in αSMA expression found in PC3‐educated MSCs may also be causative in their decreased migration capacity . Both αSMA and vimentin are structural proteins and vimentin is a mesenchymal cell marker .…”

Section: Discussionmentioning

confidence: 99%

Secreted factors from metastatic prostate cancer cells stimulate mesenchymal stem cell transition to a pro‐tumourigenic ‘activated’ state that enhances prostate cancer cell migration

Ridge

Bhattacharyya

Dervan

et al. 2018

Intl Journal of Cancer

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are a heterogeneous population of multipotent cells that are capable of differentiating into osteocytes, chondrocytes and adipocytes. Recently, MSCs have been found to home to the tumour site and engraft in the tumour stroma. However, it is not yet known whether they have a tumour promoting or suppressive function. We investigated the interaction between prostate cancer cell lines 22Rv1, DU145 and PC3, and bone marrow-derived MSCs. MSCs were 'educated' for extended periods in prostate cancer cell conditioned media and PC3-educated MSCs were found to be the most responsive with a secretory profile rich in pro-inflammatory cytokines. PC3-educated MSCs secreted increased osteopontin (OPN), interleukin-8 (IL-8) and fibroblast growth factor-2 (FGF-2) and decreased soluble fms-like tyrosine kinase-1 (sFlt-1) compared to untreated MSCs. PC3-educated MSCs showed a reduced migration and proliferation capacity that was dependent on exposure to PC3-conditioned medium. Vimentin and α-smooth muscle actin (αSMA) expression was decreased in PC3-educated MSCs compared to untreated MSCs. PC3 and DU145 education of healthy donor and prostate cancer patient-derived MSCs led to a reduced proportion of FAP+ αSMA+ cells contrary to characteristics commonly associated with cancer associated fibroblasts (CAFs). The migration of PC3 cells was increased toward both PC3-educated and DU145-educated MSCs compared to untreated MSCs, while DU145 migration was only enhanced toward patient-derived MSCs. In summary, MSCs developed an altered phenotype in response to prostate cancer conditioned medium which resulted in increased secretion of pro-inflammatory cytokines, modified functional activity and the chemoattraction of prostate cancer cells.

show abstract

Selective Migration of α-Smooth Muscle Actin-Positive Myofibroblasts toward Fibronectin in the Boyden's Blindwell Chamber

Cited by 25 publications

References 15 publications

The role of α-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling

The role of α-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling

Therapeutic Ultrasound Stimulation of Tendon Cell Migration

Secreted factors from metastatic prostate cancer cells stimulate mesenchymal stem cell transition to a pro‐tumourigenic ‘activated’ state that enhances prostate cancer cell migration

Contact Info

Product

Resources

About