Selective precipitation and characterization of lignin–carbohydrate complexes (LCCs) from Eucalyptus

Zhao, Bao-Cheng; Chen, Boyang; Cao, Xuefei; Yuan, Tong‐Qi; Wang, Shuangfei; Charlton, Adam; Sun, Run‐Cang

doi:10.1007/s00425-018-2842-9

Cited by 46 publications

(28 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phosphoric acid offers the highest yield of lignin precipitation but requires a high concentration to reach pH values lower than 4 22 . Hydrochloric acid (HCl) is frequently used in a lab-scale experiment with black liquor obtained from grass plants [23][24][25][26] . However, HCl causes corrosion to stainless steel, thus limiting its application in large-scale operation 22 .…”

mentioning

confidence: 99%

The novel method to reduce the silica content in lignin recovered from black liquor originating from rice straw

Nghị

Pham

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Difficulties in the production of lignin from rice straw because of high silica content in the recovered lignin reduce its recovery yield and applications as bio-fuel and aromatic chemicals. Therefore, the objective of this study is to develop a novel method to reduce the silica content in lignin from rice straw more effectively and selectively. The method is established by monitoring the precipitation behavior as well as the chemical structure of precipitate by single-stage acidification at different pH values of black liquor collected from the alkaline treatment of rice straw. The result illustrates the significant influence of pH on the physical and chemical properties of the precipitate and the supernatant. The simple two-step acidification of the black liquor at pilot-scale by sulfuric acid 20w/v% is applied to recover lignin at pH 9 and pH 3 and gives a percentage of silica removal as high as 94.38%. Following the developed process, the high-quality lignin could be produced from abundant rice straw at the industrial-scale.

show abstract

mentioning

confidence: 99%

The novel method to reduce the silica content in lignin recovered from black liquor originating from rice straw

Nghị

Pham

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Following these efforts, a laboratory analytical procedure for the quantification of hydroxyls in bio-oil has been developed by National Renewable Energy Laboratory 129 . Functional groups of LCCs in softwood and hardwood might be also analyzed by quantitative 31 P NMR analysis 130,131 . Biomass impurities, tricin, and tricin-like flavonoid derivatives were also successfully identified by this 31 P NMR technique in recent studies 132,133 .…”

Section: Applications Of the 31 P Nmr Protocolmentioning

confidence: 99%

Determination of hydroxyl groups in biorefinery resources via quantitative 31P NMR spectroscopy

et al. 2019

View full text Add to dashboard Cite

The analysis of chemical structural characteristics of biorefinery product streams (such as lignin and tannin) has advanced substantially over the past decade, with traditional wet-chemical techniques being replaced or supplemented by NMR methodologies. Quantitative 31 P NMR spectroscopy is a promising technique for the analysis of hydroxyl groups because of its unique characterization capability and broad potential applicability across the biorefinery research community. This protocol describes procedures for (i) the preparation/solubilization of lignin and tannin, (ii) the phosphitylation of their hydroxyl groups, (iii) NMR acquisition details, and (iv) the ensuing data analyses and means to precisely calculate the content of the different types of hydroxyl groups. Compared with traditional wet-chemical techniques, the technique of quantitative 31 P NMR spectroscopy offers unique advantages in measuring hydroxyl groups in a single spectrum with high signal resolution. The method provides complete quantitative information about the hydroxyl groups with small amounts of sample (~30 mg) within a relatively short experimental time (~30-120 min).

show abstract

“…Interestingly, UL showed the C 6 -H 6 correlation of arabinosyl units Ara-6 at 63.3/3.40 ppm and C 5 -H 5 correlations in glucosyl units Glu-5 at 73.3/3.25 ppm, respectively, but neither of these were detected in the HSQC spectra of L170. This suggests that the polysaccharides associated with lignin were dissolved and degraded during subcritical water treatment [44]. Furthermore, it is worth noting that the γ-ester structure, which exists in the lignin-carbohydrate complex (LCC), occurred in both UL and L170, meaning that the structure of γ-ester tends to stay stable during the integrated treatment [47].…”

Section: Nmr Analysis 271 2d-hsqc-nmrmentioning

confidence: 99%

Structural Changes in Milled Wood Lignin (MWL) of Chinese Quince (Chaenomeles sinensis) Fruit Subjected to Subcritical Water Treatment

et al. 2021

View full text Add to dashboard Cite

Subcritical water treatment has received considerable attention due to its cost effectiveness and environmentally friendly properties. In this investigation, Chinese quince fruits were submitted to subcritical water treatment (130, 150, and 170 °C), and the influence of treatments on the structure of milled wood lignin (MWL) was evaluated. Structural properties of these lignin samples (UL, L130, L150, and L170) were investigated by high-performance anion exchange chromatography (HPAEC), FT-IR, gel permeation chromatography (GPC), TGA, pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS), 2D-Heteronculear Single Quantum Coherence (HSQC) -NMR, and 31P-NMR. The carbohydrate analysis showed that xylose in the samples increased significantly with higher temperature, and according to molecular weight and thermal analysis, the MWLs of the pretreated residues have higher thermal stability with increased molecular weight. The spectra of 2D-NMR and 31P-NMR demonstrated that the chemical linkages in the MWLs were mainly β-O-4′ ether bonds, β-5′ and β-β′, and the units were principally G- S- H- type with small amounts of ferulic acids; these results are consistent with the results of Py-GC/MS analysis. It is believed that understanding the structural changes in MWL caused by subcritical water treatment will contribute to understanding the mechanism of subcritical water extraction, which in turn will provide a theoretical basis for developing the technology of subcritical water extraction.

show abstract

Selective precipitation and characterization of lignin–carbohydrate complexes (LCCs) from Eucalyptus

Cited by 46 publications

References 38 publications

The novel method to reduce the silica content in lignin recovered from black liquor originating from rice straw

The novel method to reduce the silica content in lignin recovered from black liquor originating from rice straw

Determination of hydroxyl groups in biorefinery resources via quantitative 31P NMR spectroscopy

Structural Changes in Milled Wood Lignin (MWL) of Chinese Quince (Chaenomeles sinensis) Fruit Subjected to Subcritical Water Treatment

Contact Info

Product

Resources

About