Selective processing of all rotational and translational optic flow directions in the zebrafish pretectum and tectum

Wang, Kun; Hinz, Julian; Haikala, Vaeinoe; Reiff, Dierk F.; Arrenberg, Aristides B.

doi:10.1186/s12915-019-0648-2

Cited by 46 publications

(72 citation statements)

References 44 publications

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“…In contrast, in the experimental group without occlusion of the non-stimulated eye, many tectal neurons were identified in the ipsilateral hemisphere as well, and these ipsilateral neurons To demonstrate how direction selectivity analysis gets compromised by this artifact, we presented motion in eight different directions using a Petri dish lid (in this study) or a glass bulb as the container for the animal (Supplementary Figure S3) 28 . Using the glass bulb, we appropriately detected direction-selective tectal neurons, which preferred either vertical or horizontal directions.…”

Section: General Reflection Disturbs Monocular Receptive Field Mappinmentioning

confidence: 99%

Reduction of visual stimulus artifacts using a spherical tank for small, aquatic animals

Wang

Arrenberg²,

Hinz

et al. 2020

Preprint

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Delivering appropriate stimuli remains a challenge in vision research, particularly for aquatic animals such as zebrafish. Due to the shape of the water tank and the associated optical paths of light rays, the stimulus can be subject to unwanted refraction or reflection artifacts, which may spoil the experiment and result in wrong conclusions. Here, we employ computer graphics simulations and calcium imaging in the zebrafish optic tectum to show, how a spherical glass container optically outperforms many previously used water containers, including Petri dish lids. We demonstrate that aquatic vision experiments suffering from total internal reflection artifacts at the water surface or at the flat container bottom may result in the erroneous detection of visual neurons with bipartite receptive fields and in the apparent absence of neurons selective for vertical motion. Our results and demonstrations will help aquatic vision neuroscientists on optimizing their stimulation setups.

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Section: General Reflection Disturbs Monocular Receptive Field Mappinmentioning

confidence: 99%

Reduction of visual stimulus artifacts using a spherical tank for small, aquatic animals

Wang

Arrenberg²,

Hinz

et al. 2020

Preprint

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“…If high spatial resolution is not required, an interesting alternative to a projector-based stimulator is one build from arrays of LEDs (e.g. Reiser and Dickinson, 2008;Wang et al, 2019 ). The main advantage of LED arrays is that they offer a more precise timing control compared to the combination of HDMI display and PC graphics card driven by software running on a desktop PC.…”

Section: Potential Issues and Technical Improvementsmentioning

confidence: 99%

An arbitrary-spectrum spatial visual stimulator for vision research

Franke

Chagas

Zhao

et al. 2019

Preprint

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Visual neuroscientists require accurate control of visual stimulation. However, few stimulator solutions simultaneously offer high spatio-temporal resolution and free control over the spectra of the light sources, because they rely on off-the-shelf technology developed for human trichromatic vision.Importantly, consumer displays fail to drive UV-shifted short wavelength-sensitive photoreceptors, which strongly contribute to visual behaviour in many animals, including mice, zebrafish and fruit flies.Moreover, many non-mammalian species feature more than three spectral photoreceptor types. Here, we present a flexible, spatial visual stimulator with up to 6 arbitrary spectrum chromatic channels. It combines a standard digital light processing engine with open source hard-and software that can be easily adapted to the experimentalist's needs. We demonstrate the capability of this general visual stimulator experimentally in the in vitro mouse retinal whole-mount and the in vivo zebrafish. Hereby, we intend starting a community effort of sharing and developing a common stimulator design.to transfer such results to other species, it is critical to keep in mind that each species is adapted to different environments and employs different strategies to survive and procreate (reviewed in Baden and Osorio, 2018 ). In vision research, classical studies often used monkeys and cats as model organisms, which with respect to visual stimuli, e.g. in terms of spatial resolution and spectral sensitivity range, have similar requirements as humans. Today, frequently used animal models --such as Drosophila , zebrafish or rodents --feature adaptations of their visual systems "outside the specifications" for human vision: For instances, all of the aforementioned species possess UV-sensitive photoreceptors, zebrafish have tetrachromatic vision, and both zebrafish and Drosophila display higher flicker fusion frequencies than most mammals (reviewed in Marshall and Arikawa, 2014 ;Boström et al., 2016 ). Still, many studies in these species use visual stimulation devices

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“…Using this method, we obtained a boundary curve with 512 data points 488 corresponding to the pixels in x/y dimensions (left-right and dorsal-ventral) for each transverse 489 plane. In-between the annotated transverse planes, the 2D curves were interpolated to receive a 490 surface that separated the tectum from the pretectum in all three dimensions (Wang et al, 2019). 491…”

Section: Anatomical Mapping 479mentioning

confidence: 99%

“…Task-relevant visual features are processed in 44 parallel channels in the brain (Nassi and Callaway, 2009), starting in the retina (Baden et al, 45 2016). The optic tectum and pretectum (superior colliculus and accessory optic system in 46 mammals) receive direct input from direction-selective retinal ganglion cells (Giolli et al, 2006;47 Hunter et al, 2013;Robles et al, 2014) and encode visual stimuli moving in different directions 48 (Wang et al, 2019). These evolutionarily ancient structures have developed to support navigation 49 and orienting behaviour in zebrafish -which do not have a visual cortex -already soon after 50 hatching in five-day-old larvae (Beck et al, 2004;Niell and Smith, 2005).…”

Section: Introduction 40mentioning

confidence: 99%

Parallel channels for motion feature extraction in the pretectum and tectum of larval zebrafish

Wang

Hinz

Zhang

et al. 2019

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16Non-cortical visual areas in vertebrate brains extract different stimulus features, such as motion, 17 object size and location, to support behavioural tasks. The optic tectum and pretectum, two 18 primary visual areas, are thought to fulfil complementary biological functions in zebrafish to 19 support prey capture and optomotor stabilisation behaviour. However, the adaptations of these 20 brain areas to behaviourally relevant stimulus statistics are unknown. Here, we used calcium 21 imaging to characterize the receptive fields of 1,926 motion-sensitive neurons in diencephalon 22 and midbrain. We show that many caudal pretectal neurons have large receptive fields (RFs), 23whereas RFs of tectal neurons are smaller and mostly size-selective. RF centres of large-size RF 24 neurons in the pretectum are predominantly located in the lower visual field, while tectal neurons 25 sample the upper-nasal visual field more densely. This tectal visual field sampling matches the 26 expected prey item locations, suggesting that the tectal magnification of the upper-nasal visual 27 field might be an adaptation to hunting behaviour. Finally, we probed optomotor responsiveness 28 and found that even relatively small stimuli drive optomotor swimming, if presented in the lower-29 temporal visual field, suggesting that the pretectum preferably samples information from this 30 region on the ground to inform optomotor behaviour. Our characterization of the parallel 31 processing channels for non-cortical motion feature extraction provides a basis for further 32 investigation into the sensorimotor transformations of the zebrafish brain and its adaptations to 33 habitat and lifestyle. 34 35Keywords 36 receptive fields, motion vision, pretectum, optic tectum, zebrafish, calcium imaging, optomotor 37 response, topography, optic flow 38 39

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Selective processing of all rotational and translational optic flow directions in the zebrafish pretectum and tectum

Cited by 46 publications

References 44 publications

Reduction of visual stimulus artifacts using a spherical tank for small, aquatic animals

Reduction of visual stimulus artifacts using a spherical tank for small, aquatic animals

An arbitrary-spectrum spatial visual stimulator for vision research

Parallel channels for motion feature extraction in the pretectum and tectum of larval zebrafish

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