Selective protection from the inhibition by EEDQ of D1 and D2 dopamine agonist-induced rotational behavior in mice

Goodale, David; Jacobi, Athole G.; Seyfried, Donald; Weiss, Benjamin

doi:10.1016/0091-3057(88)90480-7

Cited by 8 publications

(3 citation statements)

References 29 publications

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“…Lastly, the apparent behavioral impact of D1 receptor inactivation may have been an artifact caused by sulpiride incompletely protecting D2 receptors. Although frequently used to protect D2 receptors from EEDQ-induced alkylation (Cameron and Crocker 1988; Goodale et al 1988), Fuxe et al (1986) found that the striatal D2 receptors of sulpiride-pretreated adult rats exhibited a nonsignificant decline of approximately 30% after systemic EEDQ treatment (see also Crawford et al 1994). In the same study, EEDQ produced a 90% reduction of D2 receptors in nonprotected rats.…”

Section: Discussionmentioning

confidence: 99%

Behavioral effects of dopamine receptor inactivation in the caudate-putamen of preweanling rats: role of the D2 receptor

et al. 2013

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Rationale Inactivating dopamine (DA) receptors in the caudate-putamen (CPu) attenuates basal and DA agonist-induced behaviors of adult rats, while paradoxically increasing the locomotor activity of preweanling rats. Objective The purpose of this study was to determine (a) whether D1 or D2 receptor inactivation is responsible for the elevated locomotion shown by preweanling rats and (b) whether DA receptor inactivation produces a general state in which any locomotor-activating drug will cause a potentiated behavioral response. Methods DMSO or N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) was bilaterally infused into the CPu on postnatal day (PD) 17. In Experiment 1, DA receptors were selectively protected from EEDQ-induced alkylation by pretreating rats with D1 and/or D2 antagonists. On PD 18, rats received bilateral microinjections of the DA agonist R(–)-propylnorapomorphine into the dorsal CPu and locomotor activity was measured for 40 min. In subsequent experiments, the locomotion of DMSO- and EEDQ-pretreated rats was assessed after intraCPu infusions of the selective DA agonists SKF82958 and quinpirole, the partial agonist terguride, or after systemic administration of nonDAergic compounds. Results Experiment 1 showed that EEDQ's ability to enhance the locomotor activity of preweanling rats was primarily due to the inactivation of D2 receptors. Consistent with this finding, only drugs that directly or indirectly stimulated D2 receptors produced a potentiated locomotor response in EEDQ-treated rats. Conclusions These results show that DA receptor inactivation causes dramatically different behavioral effects in preweanling and adult rats, thus providing additional evidence that the D2 receptor system is not functionally mature by the end of the preweanling period.

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Section: Discussionmentioning

confidence: 99%

Behavioral effects of dopamine receptor inactivation in the caudate-putamen of preweanling rats: role of the D2 receptor

et al. 2013

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“…Further, full recovery of repeated jaw movement elicited by D, receptor agonists was achieved when only 70% ofD, DA receptors recovered (Rosengarten et al ., 1989). Finally, studies of effects of EEDQ treatment in 6-OHDA-lesioned mice showed that the recovery of behavioral effects induced by DA agonists was more rapid than the recovery of D 2 DA receptors (Goodale et al, 1988) . These results indicate the existence of spare D, and DZ DA receptors .…”

Section: Discussionmentioning

confidence: 99%

“…2-Ethoxy-lethoxycarbonyl-1,2-dihydroquinoline (EEDQ) has been reported to inactivate irreversibly several neurotransmitter receptors in vivo and in vitro, including D, and DZ DA receptors. It has been used widely to study the turnover rate of D, and D2 DA receptors (Leff et al ., 1984 ;Giorgi et al ., 1991), the relationship between DA receptor occupancy and behavioral or biochemical responses (Battaglia et al,, 1986 ;Rosengarten et al ., 1989), and the function and functional interactions between D, and DZ receptors (Arnt and Hyttel, 1988 ;Goodale et al ., 1988).…”

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confidence: 99%

Lesions of Mouse Striatum Induced by 6‐Hydroxydopamine Differentially Alter the Density, Rate of Synthesis, and Level of Gene Expression of D₁ and D₂ Dopamine Receptors

Qin

Chen

Weiss

1994

Journal of Neurochemistry

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To study the effects of altering dopaminergic input on the levels and rate of synthesis of dopamine receptors, corpora striata of mice were lesioned unilaterally with 6-hydroxydopamine (6-OHDA), and the densities and levels of the mRNAs for D, and D2 dopamine receptors were determined . The results showed that 6-OHDA caused significant reductions in D, dopamine receptors and in D, dopamine receptor mRNA in dorsolateral and dorsomedial regions of the lesioned striatum . By contrast, 6-OHDA lesions caused significant increases in D 2 dopamine receptors and in D 2 dopamine receptor mRNA in dorsolateral and ventrolateral regions of the lesioned striatum . To assess the effects of 6-OHDA lesions on the rate of synthesis of D, and D2 dopamine receptors, the irreversibly acting dopamine receptor antagonist 2-ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline (EEDQ) was administered, and the rate of recovery of these receptors determined . The lesions decreased the rate of synthesis of D, dopamine receptors in dorsolateral striatum but increased the rate of synthesis of D2 dopamine receptors in dorsolateral striatum . Correlation of these molecular events with dopaminergic behaviors showed that the rate of recovery from EEDQ-induced cataleptic activity and the recovery from inhibition of quinpirole-induced rotational behavior was more rapid than the recovery of either the D, or D 2 dopamine receptor . These results suggest that dopaminergic denervation differentially affects the rate of synthesis of D, and D2 dopamine receptors in mouse striatum, and that these alterations in the rates of synthesis of the receptors may be explained by corresponding alterations in the levels of the respective transcripts for these receptors . Key Words : In situ hybridization-Receptor autoradiography-D, and D 2 dopamine receptor-D, and D 2 dopamine receptor mRNA-Rate of synthesis-Gene expression .

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Role of dopamine receptors in the regulation of aggression in mice; relationship to genotype

1992

Neurosci Behav Physiol

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The interline differences in the manifestation of aggression evoked by stimulation was studied in mice of eight inbred lines, and the role of different types of dopamine (DA) receptors in its manifestation was investigated. Aggression was assessed in a test involving the effect of a weak electrical stimulation through the floor of the cage. A significant relationship to the animals' genotype was demonstrated, and low-aggression (C3h/He, DD, BALB/c, and AKR) and high-aggression (CBA, DBA/2, and CC57Br) lines could be distinguished on the basis of the level of aggressivity. The mixed agonist of DA receptors, apomorphine, in a one-time administration activated aggressivity in the low-aggression mice. The selective stimulation of D2-receptors with bromocriptine substantially increased the evoked aggressivity in the low-aggression mice; the blockade of D2-receptors by sulpiride decreased or prevented the manifestation of aggressivity in the high-aggression lines. At the same time, the selective D1-agonist SKF 38393 and the selective D1-antagonist SCH 23390 did not exert a substantial influence on evoked aggressivity. Evidently the D2-receptors play a key role in the control of aggression evoked by stimulation, which constitutes a model of affective aggression.

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Selective protection from the inhibition by EEDQ of D1 and D2 dopamine agonist-induced rotational behavior in mice

Cited by 8 publications

References 29 publications

Behavioral effects of dopamine receptor inactivation in the caudate-putamen of preweanling rats: role of the D2 receptor

Behavioral effects of dopamine receptor inactivation in the caudate-putamen of preweanling rats: role of the D2 receptor

Lesions of Mouse Striatum Induced by 6‐Hydroxydopamine Differentially Alter the Density, Rate of Synthesis, and Level of Gene Expression of D₁ and D₂ Dopamine Receptors

Role of dopamine receptors in the regulation of aggression in mice; relationship to genotype

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Selective protection from the inhibition by EEDQ of D1 and D2 dopamine agonist-induced rotational behavior in mice

Cited by 8 publications

References 29 publications

Behavioral effects of dopamine receptor inactivation in the caudate-putamen of preweanling rats: role of the D2 receptor

Behavioral effects of dopamine receptor inactivation in the caudate-putamen of preweanling rats: role of the D2 receptor

Lesions of Mouse Striatum Induced by 6‐Hydroxydopamine Differentially Alter the Density, Rate of Synthesis, and Level of Gene Expression of D1 and D2 Dopamine Receptors

Role of dopamine receptors in the regulation of aggression in mice; relationship to genotype

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Lesions of Mouse Striatum Induced by 6‐Hydroxydopamine Differentially Alter the Density, Rate of Synthesis, and Level of Gene Expression of D₁ and D₂ Dopamine Receptors