Selective Quenchofluorometric Detection of Fluoranthenic Polycyclic Aromatic Hydrocarbons in High-Performance Liquid Chromatography

The Gulf Oil Spill

“…Good recoveries (86-107%) were achieved, and RSD was 2.7-13.6% RSD. A quenchofluorometric detection system provides an inexpensive method to achieve selective detection for the fluoranthenic PAHs as a group (Konash et al 1981). UV detectors have been used to measure PAHs in fats and oil samples;…”

Micellar liquid chromatography of polycyclic aromatic hydrocarbons: Alkylpyridinium chloride as mobile phase modifier and selective fluorescence quencher

“…Spectroscopic interferences can be overcome in part by making the detection selective. For example, setting the excitation and emission wavelengths at their optimum values can result in selective PAH detection [10]. However, real-world samples are usually very complex and their compositions are not known; therefore, it is not practical to use this technique to provide selective PAH detection for such samples.…”

“…Selective fluorescence quenching was introduced in chromatographic detection in the 1960s [37] in thin-layer chro- matography, in the late 1970s [16] and early 1980s [10] in liquid chromatography, and in the early 1990s [20] in microcolumn liquid chromatography. Other compounds that do not suffer from inner-filter artifacts have also been identified as selective fluorescence quenching agents for PAHs.…”