Selective Recovery of<i>Xanthomonas</i>spp. from Rice Seed

Ming, Di; HuaZhi, Ye; Schaad, N. W.; Roth, Don A.

doi:10.1094/phyto-81-1358

Cited by 34 publications

(15 citation statements)

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“…The isolated xanthomonads in this study, however, did not cause diseases symptoms when artificially inoculated into the rice plant. They were frequently found in the seed samples, which supports previous findings on the association of nonpathogenic xanthomonads with rice seed (Ming et al. 1991; Cottyn et al.…”

Section: Discussionsupporting

confidence: 91%

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Phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological control

Cottyn

Debode²,

Regalado

et al. 2009

Journal of Applied Microbiology

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Aims: To study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria. Methods and Results: Seed of rice cultivar PSBRc14 was collected from farmers’ fields of irrigated lowland in southern Luzon, Philippines. Isolations of distinct colonies yielded 498 isolates. Classification of the isolates according to similarities in cellular characteristics, whole‐cell fatty acid composition, and colony appearance differentiated 101 morphotype groups. Predominant bacteria were Coryneform spp., Pantoea spp. and Pseudomonas spp. Other bacteria regularly present were Actinomycetes spp., Bacillus pumilus, B. subtilis, Burkholderia glumae, Enterobacter cloacae, Paenibacillus polymyxa, Staphylococcus spp. and Xanthomonas spp. The genetic diversity among isolates was assessed by BOX‐PCR fingerprinting of genomic DNA and represented 284 fingerprint types (FPTs). Most FPTs (78%) were not shared among samples, while eight FPTs occurred frequently in the samples. Seven of these FPTs also occurred frequently in a previous collection made from rainfed lowlands of Iloilo island, Philippines. Sixteen per cent of the isolates inhibited in vitro the mycelial growth of the rice pathogens Rhizoctonia solani and Pyricularia grisea, whereas 4% were pathogens identified as Burkholderia glumae, Burkholderia gladioli and Acidovorax avenae ssp. avenae. Conclusions: This study reveals a broad morphological and genetic diversity of bacteria present on seed of a single rice cultivar. Significance and Impact of the Study: This line of work contributes to a better understanding of the microbial diversity present on rice seed and stresses its importance as a carrier of antagonists and pathogens.

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Section: Discussionsupporting

confidence: 91%

“…Fluorescent pseudomonads were negligible on the dry seed, but appeared to proliferate on the germinated seed. Pseudomonads are commonly isolated from rice seed and are considered saprophytes (Zeigler and Alvarez 1990; Ming et al. 1991; Cottyn et al.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological control

Cottyn

Debode²,

Regalado

et al. 2009

Journal of Applied Microbiology

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“…However, this genus includes not only pathogenic strains of bacteria. Isolates of X. campestris that do not cause disease symptoms were found in epiphytic populations of various plants [2], avirulent or slightly pathogenic xanthomonads were isolated from cabbage [3], legumes [4], apple trees [2], tomatoes and peppers [5], and rice [6]. In addition, genetic studies of xanthomonads revealed the presence of a group of avirulent strains belonging to the species X. arboricola [7].…”

Section: Introductionmentioning

confidence: 99%

The role of epiphytic populations in pathogenesis of the genus Xanthomonas bacteria

2020

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Global climate warming and involvement of new regions with endemic populations of microorganisms in commercial seed production have led to an increase in the diversity of phytopathogenic bacteria that are affecting major crops, including the fruit trees. As a rule, emergence of new pathogens is associated with importation of infected seeds and planting material, cultivation of new species and varieties of plants, and expansion of agricultural trade with foreign countries. One of the leaders in diversity among phytopathogens is the genus Xanthomonas bacteria, affecting more than 400 plant species. Among the characteristic signs of xanthomonads is the high frequency of horizontal gene transfer both within the genus and between phylogenetically removed bacterial taxa – up to 25% of the genes are of this origin. In this paper, we consider another source of increasing the number of phytopathogenic species – by the epiphytic populations. These bacteria are the likely ancestral form of the phytopathogenic bacteria of the genus Xanthomonas.

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“…A detecção do patógeno depende da integração de procedimentos para liberação eficaz da bactéria alvo da semente ou outro tecido da planta e da disponibilidade de um meio que isola o patógeno alvo de microrganismos contaminantes (MING et al, 1991).…”

Section: Introductionunclassified

“…Alguns meios podem ser até mais sensíveis que técnicas imunológicas e moleculares quando se considera um baixo número de bactérias na amostra (WANG et al, 1999). oryzicola (MING;; Xanthomonas axonopodis pv. allii (ROUMAGNAC; PRUVOST, 2000).…”

Section: Introductionunclassified

>i<Xanthomonas>/i< spp. causadoras de mancha bacteriana do tomateiro (>i<Lycopersicon esculentum>/i< Mill.): detecção em sementes e diferenciação

Rabalho¹

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A minha gratidão a todos que me apoiaram e incentivaram e cuja convivência foi de extrema importância para o meu crescimento pessoal e profissional.Ao Prof. Dr. José Otavio Machado Menten, pela orientação e amizade que me proporcionaram crescimento e amadurecimento científico e, sobretudo, pela confiança depositada, dando-me a oportunidade de trabalhar ao seu lado, mesmo sem me conhecer, abrindo-me as portas da Patologia de Sementes e da Bacteriologia de plantas. À Dra. Suzete Lanza Destéfano, por me receber e orientar nos experimentos de Biologia Molecular, sempre com muita atenção e paciência.Ao Dr. Luis Otávio Beriam, por me orientar nos estudos de Sorologia. À Irene Maria Gatti de Almeida (Instituto Biológico), grande pesquisadora e amiga, pelo exemplo de dedicação e seriedade, por se preocupar e cuidar de mim em todos os momentos desde que nos conhecemos e pelas valiosas dicas nos experimentos de meio semi-seletivo e sorologia. Aos Drs. Júlio Rodrigues Neto (Instituto Biólogico), Alice Maria Quezado-Duval (EMBRAPA -CNPH), Rui S. Leite (IAPAR) e Ricardo Giória (Sakata Seeds Sudamérica Ltda) pela doação dos isolados de Xanthomonas spp. utilizados neste trabalho.Ao Prof. Paulo Cesar Tavares de Melo (ESALQ), Sakata Seeds Sudamérica Ltda e EMBRAPA -CNPH pela doação das sementes de tomate.Aos Professores do PPG Microbiologia Agrícola pelos ensinamentos transmitidos, especialmente aos Profs. Drs. Flavio C. Tavares, pela orientação no primeiro ano de curso, e Luiz Gonzaga, pela amizade e bons conselhos.Aos Professores do Setor de Fitopatologia, especialmente ao Prof. Dr. Luis Eduardo Aranha Camargo, pela orientação no Estágio Supervisionado em Docência, pelo exemplo de dedicação ao ensino e à pesquisa, disponibilização dos equipamentos do Laboratório de Genética Molecular e pelos constantes estímulos. Aos membros da banca examinadora do Exame Geral de Qualificação, Profs. Drs. Márcio R. Lambais e Sérgio Florentino Pascholati, pelas sugestões realizadas, e especialmente ao Dr. Welington Araújo pela amizade, grande estímulo e auxílio no seqüenciamento das linhagens de Xanthomonas spp.

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Selective Recovery ofXanthomonasspp. from Rice Seed

Cited by 34 publications

References 0 publications

Phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological control

Phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological control

The role of epiphytic populations in pathogenesis of the genus Xanthomonas bacteria

>i<Xanthomonas>/i< spp. causadoras de mancha bacteriana do tomateiro (>i<Lycopersicon esculentum>/i< Mill.): detecção em sementes e diferenciação

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