Selective release of overexpressed recombinant proteins from E. coli cells facilitates one-step chromatographic purification of peptide-tagged green fluorescent protein variants

Kaveh-Baghbaderani, Yasmin; Blank-Shim, Silvia A.; Koch, Tobias; Berensmeier, Sonja

doi:10.1016/j.pep.2018.07.014

Cited by 4 publications

(1 citation statement)

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“…Buffers used for preparative and analytical chromatography were filtered (0.2 μm ∅) and degassed. The cloning of the GFP‐(RH)4 variant was published earlier by our group [26, 32]. The E. coli strain BL21DE was used and incubated at 37°C in baffled flasks, at 150 rpm until an OD600 of 0.7 was achieved.…”

Section: Methodsmentioning

confidence: 99%

Purification of a peptide tagged protein via an affinity chromatographic process with underivatized silica

Rauwolf

Steegmüller

Schwaminger

et al. 2021

Engineering in Life Sciences

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Silica is widely used for chromatography resins due to its high mechanical strength, column efficiency, easy manufacturing (i.e. controlled size and porosity), and low‐cost. Despite these positive attributes to silica, it is currently used as a backbone for chromatographic resins in biotechnological downstream processing. The aim of this study is to show how the octapeptide (RH)4 can be used as peptide tag for high‐purity protein purification on bare silica. The tag possesses a high affinity to deprotonated silanol groups because the tag's arginine groups interact with the surface via an ion pairing mechanism. A chromatographic workflow to purify GFP fused with (RH)4 could be implemented. Purities were determined by SDS‐PAGE and RP‐HPLC. The equilibrium binding capacity of the fusion protein GFP‐(RH)4 on silica is 450 mg/g and the dynamic binding capacity around 3 mg/mL. One‐step purification from clarified lysate achieved a purity of 93% and a recovery of 94%. Overloading the column enhances the purity to >95%. Static experiments with different buffers showed variability of the method making the system independent from buffer choice. Our designed peptide tag allows bare silica to be utilized in preparative chromatography for downstream bioprocessing; thus, providing a cost saving factor regarding expensive surface functionalization. Underivatized silica in combination with our (RH)4 peptide tag allows the purification of proteins, in all scales, without relying on complex resins.

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Section: Methodsmentioning

confidence: 99%