Selective response of lymphocytes from Treponema pallidum-infected rabbits to mitogens and Treponema reiteri

No significant differences were noted between the responses of lymphocytes from normal and Treponema pallidum-infected rabbits to concanavalin A. When added to cultures of normal peripheral blood lymphocytes, however, sera and tissue supernatant fluids from infected rabbits were capable of suppressing the response to concanavalin A. Although immune complexes were regularly present in suppressive sera and tissue extracts, additional experiments in which both direct and indirect approaches were used indicated that immune complexes were not responsible for the observed depression. Sequential stimulation studies, done with purified glycosaminoglycan materials obtained from suppressive sera and testicular fluids, and specific absorption studies suggested that this material blocks active sites on the lectin and merely prevents recognition of the mitogen by otherwise functionally active cells.

Section: Resultscontrasting

confidence: 99%

Reappraisal of lymphocyte responsiveness to concanavalin A during experimental syphilis: evidence that glycosaminoglycans in the sera and tissues interfere ith active binding sites on the lectin and not with the lymphocytes

Baughn¹,

Musher²

1982

“…Lymphocyte function is transiently impaired in the early phase of human syphilis as well as in experimental rabbit syphilis (8,11,12,14,(17)(18)(19)(27)(28)(29). Associated with this depressed lymphocyte responsiveness is the presence of an inhibitory factor in the blood of syphilitic humans (11,12) and rabbits (14,(17)(18)(19)(27)(28)(29). The factor(s) present in blood which is responsible for the lymphocyte hyporeactivity has not been identified.…”

Section: Discussionmentioning

confidence: 99%

“…Studies on experimental syphilis in rabbits have clearly established that the lymphocyte response to mitogens and T. pallidum is depressed during the first 3 to 4 weeks of the infection. In addition, a factor is present in the blood which inhibits the blastogenic response of lymphocytes from infected and uninfected rabbits (14,(17)(18)(19)(27)(28)(29).…”

mentioning

confidence: 99%

Suppression of lymphocyte response to concanavalin A by mucopolysaccharide material from Treponema pallidum-infected rabbits

Bey¹,

Johnson²,

Fitzgerald³

1979

The testicular fluid and serum from rabbits infected intratesticularly with Treponema pallidum inhibited the mitogenic response of normal rabbit peripheral blood lymphocytes to concanavalin A. Mucopolysaccharide material present in the testicular fluid and serum was associated with the lymphocyte-inhibitory activity. Degradation of the mucopolysaccharide material with hyaluronidase resulted in the loss of the inhibitory activity of testicular fluid and serum of T. pallidu-infected rabbits.

“…Plasma or serum from patients with the early stages of the disease was found to cause reduced levels of blastogenesis of normal lymphocytes (5,6). During the first few weeks of infection, lymphocytes from T. pallidum-infected rabbits respond poorly after being cultured with concanavalin A (12) or phytohemagglutinin and pokeweed mitogen (11). However, normal lymphoblastic responses were reached 5 to 6 weeks after initial infection in these animals.…”

Section: Discussionmentioning

confidence: 99%

“…In experimentally induced disease, a reduced number of lymphocytes was detected in the spleens of neonatal rabbits infected with T. pallidum (2). Poor in vitro responses to concanavalin A (12) and phytohemagglutinin and pokeweed mitogen (11) have been reported in T. pallidum-infected rabbits. In two studies with peripheral blood leukocytes in the migration inhibition test, inhibition of migration occurred only in the later stages of T. pallidum infection (4,20).…”

mentioning

confidence: 99%

Development of of macrophage migration inhibition in rabbits infected with virulent Treponema pallidum

Pavia¹,

Folds²,

Baseman³

1977

Self Cite

Peritoneal exudate cells from rabbits infected with Treponema pallidum Nichols were used as indicators of macrophage migration inhibitory factor activity. Between 3 and 15 weeks after infection, the migration of peritoneal exudate cells was inhibited in the presence of 3 to 25 ,ug of T. phagedenis biovar Reiter protein per ml. Before this period, the migration patterns of peritoneal exudate cells from infected animals were uninhibited and similar to those from noninfected control rabbits. These observations were correlated with the development of active cellmediated immunity during experimental T. pallidum infection.