2016
DOI: 10.1098/rsob.150277
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Selectivity in glycosaminoglycan binding dictates the distribution and diffusion of fibroblast growth factors in the pericellular matrix

Abstract: The range of biological outcomes generated by many signalling proteins in development and homeostasis is increased by their interactions with glycosaminoglycans, particularly heparan sulfate (HS). This interaction controls the localization and movement of these signalling proteins, but whether such control depends on the specificity of the interactions is not known. We used five fibroblast growth factors with an N-terminal HaloTag (Halo-FGFs) for fluorescent labelling, with well-characterized and distinct HS-b… Show more

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Cited by 23 publications
(18 citation statements)
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“…These FGFR splice variants and cofactor molecules, which include heparan sulfate proteoglycans and Klotho-family proteins, also determine the strength and specificity of ligand binding and receptor activation (Ornitz, 2000; Polanska et al, 2009; Itoh et al, 2015). Binding to heparan sulfate also serves to limit FGF diffusion through tissue (Sun et al, 2016). …”
Section: Introductionmentioning
confidence: 99%
“…These FGFR splice variants and cofactor molecules, which include heparan sulfate proteoglycans and Klotho-family proteins, also determine the strength and specificity of ligand binding and receptor activation (Ornitz, 2000; Polanska et al, 2009; Itoh et al, 2015). Binding to heparan sulfate also serves to limit FGF diffusion through tissue (Sun et al, 2016). …”
Section: Introductionmentioning
confidence: 99%
“…In analogous experiments, Fernig and his colleagues used five Halo-tagged fibroblast growth factors with well-characterized HS-binding properties, and measured their binding and diffusion within the ECM produced by mammary fibroblasts. [43] Halo-FGF-1, FGF-2, and FGF-6 bound to HS, but FGF-20 did not. Their relative distributions were not uniform either; FGF-2 and FGF-6 diffused more rapidly, whereas FGF-1 lagged, and FGF-10 remained stationary.…”
Section: Hs Codesmentioning
confidence: 90%
“…In analogous experiments, Fernig and his colleagues used five Halo‐tagged fibroblast growth factors with well‐characterized HS‐binding properties, and measured their binding and diffusion within the ECM produced by mammary fibroblasts . Halo‐FGF‐1, FGF‐2, and FGF‐6 bound to HS, but FGF‐20 did not.…”
Section: Hs Codesmentioning
confidence: 99%
“…These proteins include growth factors, cytokines, chemokines, morphogens, enzymes, enzyme inhibitors, receptors, and extracellular matrix proteins, forming the network of HS-binding proteins, termed the heparan sulfate interactome [2]. Binding to the polysaccharide may, for example, regulate ligand diffusion [3,4], formation of signalling ligand-receptor complexes [5,6] and enzyme activity [7].…”
Section: Ii-introductionmentioning
confidence: 99%
“…P&L: FGF-2 reacted in solution with PGO when bound to a heparin affinity column (protection step) and then, following elution, reaction of any protected arginine residues with HPG (labelling step) 4. The bolded amino acids are a part of the known HBSs which were noted in column 3 rd of FGF-2 on column.…”
mentioning
confidence: 99%