Seleksi Padi dengan Kandungan Amilosa Sedang Menggunakan Marka Molekuler dan Pengujian Fenotipik

Abstract

Rice with medium amylose content is a special type of rice that highly demanded by the people in Asia and world. The purpose of this study was to obtain individual F7 progeny (derived from a cross between Sintanur x PTB33 and Pandanwangi x PTB33) with medium amylose content. The materials used were spectrophotometer with wave lenght 625 nm, meanwhile molecular markers applied were RM190, SSIIa and Wx. The results of this study showed that SP73-3-1-7 have most plant with medium amylose content based on molecular and phenotypic marker. The combination of both tests provides more accurate in selecting physicochemical trait in rice.Abstrak: Padi dengan kandungan amilosa sedang sangat diminati oleh masyarakat Asia dan Dunia. Tujuan dari penelitian ini adalah untuk memperoleh individu keturunan F7 (yang berasal dari hasil persilangan Sintanur x PTB33 dan Pandanwangi x PTB33) dengan kandungan amilosa sedang. Alat yang digunakan untuk analisis kandungan amilosa yaitu spektrofotometer dengan panjang gelombang 625 nm, sedangkan marka molekuler menggunakan marka RM190, SSIIa dan Wx. Hasil dari penelitian ini menunjukkan bahwa SP73-3-1-7 memiliki jumlah tanaman paling banyak yang mengandung amilosa yang sedang berdasarkan marka molekuler dan fenotipik. Kombinasi kedua pengujian ini akurat dalam menyeleksi karakter fisikokimia padi.

Validation of molecular markers associated with amylose content of F8 and F9 lines resulting from a crossing of Black Rice and Mentik Wangi

“…Rice with moderate amylose content (20-24%) is in great demand by farmers and the public because of its high selling value in local and international markets. Breeders must develop rice breeding methods with appropriate amylose content to meet consumer demand and improve farmer welfare [7].…”