Selenate bioaccumulation and toxicity in Chlamydomonas reinhardtii: Influence of ambient sulphate ion concentration

Fournier, Elodie; Adam-Guillermin, Christelle; Potin‐Gautier, Martine; Pannier, Florence

doi:10.1016/j.aquatox.2009.12.003

Cited by 60 publications

(55 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Selenate is accumulated through carrier-mediated processes in algal and bacterial cells; therefore, its uptake can be influenced by the presence of other ions, particularly sulfate, as they compete for transport sites on cell membranes (Fournier et al 2010;Maher et al 2010). Sulfate concentrations (as mg·L -1 of SO 4 − ), measured in the study streams in October of 2011 (Kuchapski 2013) were compared with total Se concentrations (g·L -1 ), and the mean ratios of SO 4 − to total Se were approximately 41:1 in reference streams and approximately 6:1 in mine-affected streams in the Elk River watershed and approximately 20:1 in both reference and mineaffected streams in the McLeod River watershed.…”

Section: Se Transfer In Lotic Food Chainsmentioning

confidence: 98%

Food chain transfer and exposure effects of selenium in salmonid fish communities in two watersheds in the Canadian Rocky Mountains

Kuchapski

Rasmussen

2015

Can. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

Selenium (Se) concentrations and fish biomass were examined in streams in two mined watersheds to determine if juvenile salmonids reflect local Se exposure concentrations downstream of surface coal mines and to investigate the relationship between Se exposure and toxicity effects at the fish community level. Se concentrations were measured in water, biofilm, macroinvertebrates, and muscle tissues from juvenile westslope cutthroat trout (Oncorhynchus clarkii lewisi), bull trout (Salvelinus confluentus), rainbow trout (Oncorhynchus mykiss), and brook trout (Salvelinus fontinalis) in mine-affected and reference streams. Significant positive Se transfer relationships were found at each measured level of the lotic food chain.

show abstract

Section: Se Transfer In Lotic Food Chainsmentioning

confidence: 98%

Food chain transfer and exposure effects of selenium in salmonid fish communities in two watersheds in the Canadian Rocky Mountains

Kuchapski

Rasmussen

2015

Can. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

show abstract

“…Many studies concerning Se toxicity in microalgae can be found in the literature; selenate effect has been studied in Chlamydomonas reinhardtii [1, 24–26], Scenedesmus quadricauda [27, 28], and cyanobacterium Spirulina platensis [29]. Similar studies have been done with Chlorella zofingiensis with emphasis on heat-stable selenoproteins [30], on metabolism of Se volatile compounds [14], Se effect on Chlorella sp.…”

Section: Introductionmentioning

confidence: 99%

Effect of Selenate on Viability and Selenomethionine Accumulation ofChlorella sorokinianaGrown in Batch Culture

Gojkovic

Vílchez

Torronteras

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

The aim of this work was to study the effect of Se(+VI) on viability, cell morphology, and selenomethionine accumulation of the green alga Chlorella sorokiniana grown in batch cultures. Culture exposed to sublethal Se concentrations of 40 mg·L−1 (212 μM) decreased growth rates for about 25% compared to control. A selenate EC50 value of 45 mg·L−1 (238.2 μM) was determined. Results showed that chlorophyll and carotenoids contents were not affected by Se exposure, while oxygen evolution decreased by half. Ultrastructural studies revealed granular stroma, fingerprint-like appearance of thylakoids which did not compromise cell activity. Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. Results revealed that the microalga was able to accumulate up to 140 mg·kg−1 of SeMet in 120 h of cultivation. This paper shows that Chlorella sorokiniana biomass can be enriched in the high value aminoacid SeMet in batch cultures, while keeping photochemical viability and carbon dioxide fixation activity intact, if exposed to suitable sublethal concentrations of Se.

show abstract

“…Because to its chemical similarity with sulfate, the mechanism of selenate uptake should be similar and a competitive mechanism for cell assimilation may also exist (Fournier et al 2010). Selenate may be reduced by some botanical compounds and metabolized to form new compounds, e.g., amino acids and low molecular species such as selenomethionine, selenocysteine, selenoglutatione, and other compounds such as selenoproteins or selenoenzymes.…”

mentioning

confidence: 99%

Effects of the Addition of Selenium on Trace Element Concentrations in Danshen (Salvia miltiorrhiza)

Dong

Liu

2014

Analytical Letters

View full text Add to dashboard Cite

Danshen (Salvia miltiorrhiza), a Chinese herbal medicine, was cultivated under three conditions of selenium enrichment. Fifteen trace and minor elements were determined in different tissues of the plant by inductively coupled plasma-mass spectrometry and hydride generation-atomic fluorescence spectrometry after microwave digestion. The goals of this work were to investigate the concentrations of trace elements in Danshen and determine whether the element concentrations of As, Cd, Hg, Pb, and Cu exceeded the safety standards; to compare the concentrations of fifteen elements in roots, stems, and leaves of Danshen under the same selenium enrichment level; to compare the concentrations of fifteen elements in the same tissue of Danshen under different selenium enrichment levels; and to compare the translocation factors after selenium enrichment. The results showed that the concentrations of elements As, Cd, Hg, Pb, and Cu in roots were lower than the specified values. In general, under the same selenium enrichment conditions, the elemental concentrations in roots were lower than those in stems and leaves. Furthermore, most element concentrations in leaves were higher than those in stems. The concentrations of Pb, Cr, and Hg in roots decreased while other elemental concentrations increased after selenium enrichment. Cu, Pb, Cd, Cr, and Hg were primarily transferred to stems and leaves when cultivated under selenium enrichment.

show abstract

Selenate bioaccumulation and toxicity in Chlamydomonas reinhardtii: Influence of ambient sulphate ion concentration

Cited by 60 publications

References 25 publications

Food chain transfer and exposure effects of selenium in salmonid fish communities in two watersheds in the Canadian Rocky Mountains

Food chain transfer and exposure effects of selenium in salmonid fish communities in two watersheds in the Canadian Rocky Mountains

Effect of Selenate on Viability and Selenomethionine Accumulation ofChlorella sorokinianaGrown in Batch Culture

Effects of the Addition of Selenium on Trace Element Concentrations in Danshen (Salvia miltiorrhiza)

Contact Info

Product

Resources

About