Selenide-linked Polydopamine-Reinforced Hybrid Hydrogels with On-demand Degradation and Light-triggered Nanozyme Release for Diabetic Wound Healing

Abstract

Background Multifunctional hydrogels with controllable degradation and drug release have attracted extensive attention in diabetic wound healing. This study focused on the acceleration of diabetic wound healing with selenide-linked polydopamine-reinforced hybrid hydrogels with on-demand degradation and light-triggered nanozyme release. Methods Herein, selenium-containing hybrid hydrogels, defined as DSeP@PB, were fabricated via the reinforcement of selenol-end capping polyethylene glycol (PEG) hydrogels by polydopamine nanoparticles (PDANPs) and Prussian blue nanozymes in a one-pot approach in the absence of any other chemical additive or organic solvent based on diselenide and selenide bonding-guided crosslinking, making them accessible for large-scale mass production. Results Reinforcement by PDANPs greatly increases the mechanical properties of the hydrogels, realizing excellent injectability and flexible mechanical properties for DSeP@PB. Dynamic diselenide introduction endowed the hydrogels with on-demand degradation under reducing or oxidizing conditions and light-triggered nanozyme release. The bioactivity of Prussian blue nanozymes afforded the hydrogels with efficient antibacterial, ROS-scavenging and immunomodulatory effects, which protected cells from oxidative damage and reduced inflammation. Further animal studies indicated that DSeP@PB under red light irradiation showed the most efficient wound healing activity by stimulating angiogenesis and collagen deposition and inhibiting inflammation. Conclusion The combined merits of DSeP@PB (on-demand degradation, light-triggered release, flexible mechanical robustness, antibacterial, ROS-scavenging and immunomodulatory capacities) enable its high potential as a new hydrogel dressing that can be harnessed for safe and efficient therapeutics for diabetic wound healing.

Selenol-ene chemistry-based one pot reaction for unsymmetric selenides synthesis and rate regulation of selenide oxidative elimination

“…20 We have reported a facile one-pot synthesis of selenide, in which selenol is obtained from the ammonolysis of SBL followed by a Michael addition reaction to conjugated alkenes. 5,15 Due to the high reactivity of selenols, a variety of b-site unsymmetric selenides containing ester and amide groups can be prepared. At the same time, by incorporating only a minimal amount of catalyst, unsymmetric selenides with various blocking groups can be easily obtained in high yields.…”

“…2 Selenides have received much attention since their dynamic nature endows the materials with striking sensitivity under optimum environmental alterations, such as redox, light and irradiation. [3][4][5][6][7] As reported by Sharpless in 1973, a kind of selenide containing electron withdrawing groups, the a-phenylselenocarbonyl compound, was easily oxidized to afford selenoxides and subsequently underwent spontaneous syn elimination to generate an a,b-unsaturated ketone. 8,9 Structural changes in the selenide moiety under oxidative stimulation can cause dramatic changes in the material properties.…”

Metal nanoparticle hybrid hydrogels: the state-of-the-art of combining hard and soft materials to promote wound healing

“…Nanomaterials have a wide range of biological activities, of which metal nanoparticles are outstanding representatives [25][26][27][28][29][30]. Metal nanoparticles are three-dimensional crystalline materials with diameters ranging from 1 to 100 nm.…”