Selenite Enhances Immune Response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans

Li, Wen-Hsuan; Chang, Chun‐Han; Huang, Chi‐Wei; Wei, Chia-Cheng; Liao, Vivian Hsiu‐Chuan

doi:10.1371/journal.pone.0105810

Cited by 18 publications

(15 citation statements)

References 57 publications

(94 reference statements)

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“…This would mimic the effects exerted by a marine brown seaweed extract in overcoming the pathogenicity of PA14 towards C. elegans (Kandasamy et al, 2012). Alternately, H. atra could also contain compounds capable of directly inducing lys-7 activities, as reported elsewhere (Fatin et al, 2017; Li et al, 2014).…”

Section: Discussionsupporting

confidence: 56%

Black sea cucumber (Holothuria atra Jaeger, 1833) rescues Pseudomonas aeruginosa-infected Caenorhabditis elegans via reduction of pathogen virulence factors and enhancement of host immunity

Lee

Tan

Eng

et al. 2019

Preprint

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Materials and methods:Here, we utilized the Caenorhabditis elegans-P. aeruginosa 43 infection model to assess the anti-infective properties of H. atra. We conducted the C. 44 elegans-P. aeruginosa slow killing assay to isolate a fraction with capable of conferring 45 survival to pathogen-infected worms, followed by a series of tests to assess the 46 anti-virulence and immune-related properties of the sea cucumber bioactive fraction. 47 Results: The H. atra methanol extract improved survival of infected worms without 48 affecting pathogen viability. Further extraction and assays sequestered a fraction with 49 significant anti-infective activities and was able to attenuate the secretion of virulence 50 factors and biofilm formation. Treatment of P. aeruginosa-infected transgenic lys-7::GFP 51 worms with this fraction also restored the repressed expression of host lys-7, a defense 52 enzyme. QTOF-LCMS analysis of this fraction revealed the presence of 53 aspidospermatidine, an indole alkaloid and inosine. 54 Conclusion: Collectively, results shows that H. atra confers survival advantage against P. 55 aeruginosa infection through inhibition of pathogen virulence and the restitution of host 56 lys-7 expression.57

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Section: Discussionsupporting

confidence: 56%

Black sea cucumber (Holothuria atra Jaeger, 1833) rescues Pseudomonas aeruginosa-infected Caenorhabditis elegans via reduction of pathogen virulence factors and enhancement of host immunity

Lee

Tan

Eng

et al. 2019

Preprint

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“…Previous work has shown that skn‐1 mutants are vulnerable to oxidative stress, whereas skn‐1 overexpression afforded protection against manganese‐induced toxicity . Furthermore, selenite exposure of the nematode has been shown to enhance expression of gst‐4 as well as gcs‐1 under PA14 infection via SKN‐1 . Recently, Salgueiro et al.…”

Section: Resultsmentioning

confidence: 96%

Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems

Rohn

Raschke

Aschner

et al. 2019

Molecular Nutrition Food Res

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Scope: Small selenium (Se) species play a key role in Se metabolism and act as dietary sources of the essential trace element. However, they are redox-active and trigger pro-and antioxidant responses. As health outcomes are strongly species-dependent, species-specific characteristics of Se compounds are tested in vivo. Methods and results: In the model organism Caenorhabditis elegans (C. elegans), immediate and sustained effects of selenite, selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) are studied regarding their bioavailability, incorporation into proteins, as well as modulation of the cellular redox status. While all tested Se compounds are bioavailable, only SeMet persistently accumulates and is non-specifically incorporated into proteins. However, the protection toward chemically-induced formation of reactive species is independent of the applied Se compound. Increased thioredoxin reductase (TXNRD) activity and changes in mRNA expression levels of antioxidant proteins indicate the activation of cellular defense mechanisms. However, in txnrd-1 deletion mutants, no protective effects of the Se species are observed anymore, which is also reflected by differential gene expression data. Conclusion: Se species protect against chemically-induced reactive species formation. The identified immediate and sustained systemic effects of Se species give rise to speculations on possible benefits facing subsequent periods of inadequate Se intake.

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“…In C. elegans selenite exerts beneficial effects on development, cholinergic signaling, and innate immune response (Li et al, 2011;Li et al, 2014a). At high concentrations, selenite can be harmful to C. elegans (Morgan et al, 2010;Estevez et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

HIF-1 Has a Central Role in Caenorhabditis elegans Organismal Response to Selenium

et al. 2020

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Selenium is a trace element for most organisms; its deficiency and excess are detrimental. Selenium beneficial effects are mainly due to the role of the 21 st genetically encoded amino acid selenocysteine (Sec). Selenium also exerts Sec-independent beneficial effects. Its harmful effects are thought to be mainly due to non-specific incorporation in protein synthesis. Yet the selenium response in animals is poorly understood. In Caenorhabditis elegans, Sec is genetically incorporated into a single selenoprotein. Similar to mammals, a 20-fold excess of the optimal selenium requirement is harmful. Sodium selenite (Na 2 SeO 3) excess causes development retardation, impaired growth, and neurodegeneration of motor neurons. To study the organismal response to selenium we performed a genetic screen for C. elegans mutants that are resistant to selenite. We isolated nonsense and missense egl-9/EGLN mutants that confer robust resistance to selenium. In contrast, hif-1/HIF null mutant was highly sensitive to selenium, establishing a role for this transcription factor in the selenium response. We showed that EGL-9 regulates HIF-1 activity through VHL-1, and identified CYSL-1 as a key sensor that transduces the selenium signal. Finally, we showed that the key enzymes involved in sulfide and sulfite stress (sulfide quinone oxidoreductase and sulfite oxidase) are not required for selenium resistance. In contrast, knockout strains in the persulfide dioxygenase ETHE-1 and the sulfurtransferase MPST-7 affect the organismal response to selenium. In sum, our results identified a transcriptional pathway as well as enzymes possibly involved in the organismal selenium response.

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Selenite Enhances Immune Response against Pseudomonas aeruginosa PA14 via SKN-1 in Caenorhabditis elegans

Cited by 18 publications

References 57 publications

Black sea cucumber (Holothuria atra Jaeger, 1833) rescues Pseudomonas aeruginosa-infected Caenorhabditis elegans via reduction of pathogen virulence factors and enhancement of host immunity

Black sea cucumber (Holothuria atra Jaeger, 1833) rescues Pseudomonas aeruginosa-infected Caenorhabditis elegans via reduction of pathogen virulence factors and enhancement of host immunity

Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems

HIF-1 Has a Central Role in Caenorhabditis elegans Organismal Response to Selenium

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