Selenite supplementation modulates the hepatic metabolic sensors AMPK and SIRT1 in binge drinking exposed adolescent rats by avoiding oxidative stress

Abstract

Oxidative metabolism of ethanol after exposure to BD in hepatocytes, and its relationship with SIRT1 and AMPK through EROS and NADH/NAD+. Effects of selenium supplementation.

Adipose tissue homeostasis orchestrates the oxidative, energetic, metabolic and endocrine disruption induced by binge drinking in adolescent rats

“…A study has confirmed that BD alters insulin production, causing IR, inhibiting glucose transport and decreasing exposure to it, making an increase in insulin secretion necessary (Shelmet et al., 1988). Recently, in male adolescent rats exposed to the same experimental intermittent BD protocol as that used in the present study, a downregulation of IRS‐1 in the liver and skeletal muscle was described (Nogales et al., 2021; Romero‐Herrera et al., 2023). Therefore, during adolescence in rats, insulin does not actively reach these tissues and, consequently, its physiological action decreases.…”

“…BD exposure during adolescence in rats leads to a dangerous profile of catabolic AMPK and SIRT‐1 expression in WAT, which together with their anabolic profile found in the liver of BD adolescent rats (Nogales et al., 2021), triggers the AT–liver axis, exacerbating WAT lipolysis and increasing the amount of circulating FFA. All of this could contribute to liver steatosis accompanied by an IR process, comprising a clear risk factor for the development of future cardiovascular diseases.…”

“…However, little is known about the effects of intermittent BD exposure during adolescence in this axis and the response of AT to insulin. In this context, our research group has described that adolescent rats exposed to intermittent BD exposure exhibit lower AMP activated protein kinase (AMPK) and NAD + ‐dependent deacetylase sirtuin‐1 (SIRT‐1) hepatic expression (Nogales et al., 2021). Both proteins are sensors of the cellular energy status.…”

“…When downregulated, they are implicated in the development of steatosis by increasing lipogenesis and avoiding lipolysis (Jiang et al., 2015). Moreover, liver SIRT‐1 and AMPK depletion, via different mechanisms, inhibits some insulin signalling steps, contributing to a process of insulin resistance (IR) in BD exposed rats (Nogales et al., 2021).…”

Increased SIRT1 Concentration Following Four Years of Selenium and Q10 Intervention Associated with Reduced Cardiovascular Mortality at 10-Year Follow-Up—Sub-Study of a Previous Prospective Double-Blind Placebo-Controlled Randomized Clinical Trial

“…The downregulation of sirtuins was observed in peripheral blood mononuclear cells in CVD patients with Se deficiency [ 19 ]. Recently, increased liver expression of SIRT1 was reported in binge drinking rats when supplemented with Se [ 25 ]. Knowledge on the effect of coenzyme Q 10 on SIRT1 is also limited, although the Q 10 status seems to influence SIRT1 activity [ 26 ], as the decline in hepatic SIRT1 expression in diabetic rats was reversed by coenzyme Q 10 administration [ 27 ].…”