Selenium-dependent growth of Treponema denticola : evidence for a clostridial-type glycine reductase

Rother, Michael; Böck, August; Wyss, C.

doi:10.1007/s002030100351

Cited by 24 publications

(16 citation statements)

References 22 publications

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“…Previous assessment of the nutritional requirements of T. denticola revealed a strict growth dependence on selenium (41). Genome sequence reveals the complete set of genes for selenoprotein synthesis: selA, (TDE2477), selD (TDE2461), and selB (TDE1963).…”

Section: Resultsmentioning

confidence: 99%

Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes

Seshadri

Myers

Tettelin

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

254

314

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We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive and cooperative measures, consistent with its pathogenic nature and lifestyle within the mixed-species environment of subgingival dental plaque. Comparisons with previously sequenced spirochete genomes revealed specific factors contributing to differences and similarities in spirochete physiology as well as pathogenic potential. The T. denticola genome is considerably larger in size than the genome of the related syphiliscausing spirochete Treponema pallidum. The differences in gene content appear to be attributable to a combination of three phenomena: genome reduction, lineage-specific expansions, and horizontal gene transfer. Genes lost due to reductive evolution appear to be largely involved in metabolism and transport, whereas some of the genes that have arisen due to lineage-specific expansions are implicated in various pathogenic interactions, and genes acquired via horizontal gene transfer are largely phagerelated or of unknown function.

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Section: Resultsmentioning

confidence: 99%

Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes

Seshadri

Myers

Tettelin

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

254

314

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“…Relatively little is known regarding the metabolism of T. denticola, and many of its metabolic pathways are likely to be novel (Rother et al, 2001;Seshadri et al, 2004;Chu et al, 2008). It primarily utilizes serine, alanine, cysteine, and glycine when grown in vitro and generates fermentation products including acetate, lactate, succinate, formate, pyruvate, ethanol, carbon dioxide, hydrogen sulfide (H 2 S), and ammonia (Hespell and Canale-Parola, 1971).…”

Section: Metabolic End-productsmentioning

confidence: 99%

Virulence Factors of the Oral Spirochete Treponema denticola

et al. 2010

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There is compelling evidence that treponemes are involved in the etiology of several chronic diseases, including chronic periodontitis as well as other forms of periodontal disease. There are interesting parallels with other chronic diseases caused by treponemes that may indicate similar virulence characteristics. Chronic periodontitis is a polymicrobial disease, and recent animal studies indicate that co-infection of Treponema denticola with other periodontal pathogens can enhance alveolar bone resorption. The bacterium has a suite of molecular determinants that could enable it to cause tissue damage and subvert the host immune response. In addition to this, it has several non-classic virulence determinants that enable it to interact with other pathogenic bacteria and the host in ways that are likely to promote disease progression. Recent advances, especially in molecular-based methodologies, have greatly improved our knowledge of this bacterium and its role in disease.

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“…They involve the coupled fermentation of amino acids in which one amino acid is oxidized (Stickland donor) and another (Stickland acceptor) is reduced (29). Treponema denticola, an established resident of the oral cavity, performs Stickland reactions via the selenoprotein glycine reductase (32). Glycine reductase is composed of a multiprotein complex that contains two separate selenoproteins, termed selenoprotein A and selenoprotein B (1,7,8,15,16).…”

mentioning

confidence: 99%

“…Sequence analysis indicates the presence of several selenoproteins in addition to glycine reductase within the genome of T. denticola (24). This organism exhibits a strict growth requirement for selenium (32).…”

mentioning

confidence: 99%