Selenium enhances the expression of miR-9, miR-124 and miR-29a during neural differentiation of bone marrow mesenchymal stem cells

Jalali, Hanieh; Golchin, Hasti; Sadri, Zahra; Bardei, Latifeh Karimzadeh; Nabiuni, Mohammad

doi:10.1016/j.jtemb.2021.126898

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…Of the five chemicals used in this study, single exposure to Se reduced NSph size; Se is known to be cytotoxic, especially to cancer cells (Olm et al, 2009). On the other hand, rat bone marrow stem cells have been reported to promote neuronal differentiation at low concentrations of Se exposure but to cause cell death at high concentrations (Jalali et al, 2022). These results are similar to those of combined exposure to the five chemicals in human NSphs.…”

Section: Discussionsupporting

confidence: 66%

Epigenetic mutagen-like environmental chemicals alter neural differentiation of human induced pluripotent stem cells

Arai,

Nishino

2023

J. Toxicol. Sci.

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Various chemicals, including pesticides, heavy metals, and metabolites of tobacco, have been detected in fetal environment. Fetuses are exposed to these chemicals at relatively low concentrations; however, their risk of developing neurological and behavioral disorders increases after birth. We aimed to evaluate the effects of five chemicals (diethylphosphate, cotinine, octachlorodipropyl ether, mercury, and selenium) detected in the serum of pregnant mothers on neural development using human neurospheres (NSphs) differentiated from induced pluripotent stem cells. Exposure to each chemical at serum concentrations revealed no effects on NSph development. However, combined exposure to the five chemicals caused a significant decrease in NSph size and altered gene expression and neural differentiation. Thus, we next focused on DNA methylation to investigate changes in NSph properties caused by chemical exposure. Combined exposure to chemicals had extremely small effects on the DNA methylation status of NSphs at individual gene loci. However, stochastic changes in methylation status caused by chemical exposure were significantly accumulated throughout the entire genome. These results suggest that the five chemicals acted as epimutagens that alter the epigenetic status during human neural development at the biological level. Taken together, we showed for the first time, the epimutagen-induced alterations in neural differentiation at serum concentrations using an in vitro human neuronal model.

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Section: Discussionsupporting

confidence: 66%

Epigenetic mutagen-like environmental chemicals alter neural differentiation of human induced pluripotent stem cells

Arai,

Nishino

2023

J. Toxicol. Sci.

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“…Its high sensitivity and noninvasive nature make FLIM indispensable in basic research and clinical applications. FLIM has been employed to study the interactions between various proteins involved in forming neuronal synapses [150,151]. For instance, researchers labeled synaptic proteins with Fluorescence resonance energy transfer (FRET) pairs and applied FLIM to estimate the fluorescence lifetime of the FRET pairs.…”

Section: Medical Applicationsmentioning

confidence: 99%

Fluorescence in depth: integration of spectroscopy and imaging with Raman, IR, and CD for advanced research

Aeindartehran,

Sadri,

Rahimi

et al. 2024

Methods Appl. Fluoresc.

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Fluorescence spectroscopy serves as a vital technique for studying the interaction between light and fluorescent molecules. It encompasses a range of methods, each presenting unique advantages and applications. This technique finds utility in various chemical studies. This review discusses Fluorescence spectroscopy, its branches such as Time-Resolved Fluorescence Spectroscopy (TRFS) and Fluorescence Lifetime Imaging Microscopy (FLIM), and their integration with other spectroscopic methods, including Raman, Infrared (IR), and Circular Dichroism (CD) spectroscopies. By delving into these methods, we aim to provide a comprehensive understanding of the capabilities and significance of fluorescence spectroscopy in scientific research, highlighting its diverse applications and the enhanced understanding it brings when combined with other spectroscopic methods. This review looks at each technique's unique features and applications. It discusses the prospects of their combined use in advancing scientific understanding and applications across various domains.

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“…We found that approximately 50% (n = 63) of miR-970, 70% (n = 70) of miR-124, 65% (n = 52) of miR-986, and 83% (n = 60) of miR-957 mutant germaria contained extra CB-like cells, while the incidence in the controls was less than 10% (Fig 1D). Of these miRNAs, miR-124 was of special interest because it is conserved among vertebrates and invertebrates [46][47][48] and has a number of known functions, including roles in Drosophila neuroblast proliferation, courtship, circadian locomotor, and rhythmic behaviors [49][50][51][52], as well as the inhibition of differentiation of mouse bone marrow mesenchymal stem cells and pre-cartilaginous stem cells [53,54].…”

Section: A Mirna Screen Identifies Gsc Fate Regulatorsmentioning

confidence: 99%

Communication between the stem cell niche and an adjacent differentiation niche through miRNA and EGFR signaling orchestrates exit from the stem cell state in the Drosophila ovary

Chen,

Li,

Sheng

et al. 2024

PLoS Biol

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The signaling environment, or niche, often governs the initial difference in behavior of an adult stem cell and a derivative that initiates a path towards differentiation. The transition between an instructive stem cell niche and differentiation niche must generally have single-cell resolution, suggesting that multiple mechanisms might be necessary to sharpen the transition. Here, we examined the Drosophila ovary and found that Cap cells, which are key constituents of the germline stem cell (GSC) niche, express a conserved microRNA (miR-124). Surprisingly, loss of miR-124 activity in Cap cells leads to a defect in differentiation of GSC derivatives. We present evidence that the direct functional target of miR-124 in Cap cells is the epidermal growth factor receptor (EGFR) and that failure to limit EGFR expression leads to the ectopic expression of a key anti-differentiation BMP signal in neighboring somatic escort cells (ECs), which constitute a differentiation niche. We further found that Notch signaling connects EFGR activity in Cap cells to BMP expression in ECs. We deduce that the stem cell niche communicates with the differentiation niche through a mechanism that begins with the selective expression of a specific microRNA and culminates in the suppression of the major anti-differentiation signal in neighboring cells, with the functionally important overall role of sharpening the spatial distinction between self-renewal and differentiation environments.

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Selenium enhances the expression of miR-9, miR-124 and miR-29a during neural differentiation of bone marrow mesenchymal stem cells

Cited by 5 publications

References 33 publications

Epigenetic mutagen-like environmental chemicals alter neural differentiation of human induced pluripotent stem cells

Epigenetic mutagen-like environmental chemicals alter neural differentiation of human induced pluripotent stem cells

Fluorescence in depth: integration of spectroscopy and imaging with Raman, IR, and CD for advanced research

Communication between the stem cell niche and an adjacent differentiation niche through miRNA and EGFR signaling orchestrates exit from the stem cell state in the Drosophila ovary

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