Selenium-Enriched Agaricus bisporus Mushroom Protects against Increase in Gut Permeability ex vivo and Up-Regulates Glutathione Peroxidase 1 and 2 in Hyperthermally-Induced Oxidative Stress in Rats

Abstract

Dietary effects of organic Se supplementation in the form of Se-enriched Agaricus bisporus mushroom on ileal mucosal permeability and antioxidant selenoenzymes status in heat induced oxidative stress in rats were evaluated. Acute heat stress (40 °C, 21% relative humidity, 90 min exposure) increased ileum baseline short circuit current (Isc; 2.40-fold) and epithelial conductance (Ge; 2.74-fold). Dietary supplementation with Se-enriched A. bisporus (1 µg Se/g feed) reduced (p < 0.05) ileum Isc and Ge during heat stress to 1.74 and 1.91 fold, respectively, indicating protection from heat stress-induced mucosal permeability increase. The expression of ileum glutathione peroxidase (GPx-) 1 and 2 mRNAs were up-regulated (p < 0.05) by 1.90 and 1.87-fold, respectively, for non-heat stress rats on the Se-enriched diet relative to the control. The interplay between heat stress and dietary Se is complex. For rats on the control diet, heat stress alone increased ileum expression of GPx-1 (2.33-fold) and GPx-2 (2.23-fold) relative to thermoneutral conditions. For rats on the Se-enriched diet, heat stress increased (p < 0.05) GPx-1 expression only. Rats on Se-enriched + α-tocopherol diet exhibited increased expression of both genes (p < 0.05). Thus, dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress.

Selenium and vitamin E together improve intestinal epithelial barrier function and alleviate oxidative stress in heat‐stressed pigs

“…Se and 300 mg kg −1 VE alleviated the increase of epithelial conductance in heat‐stressed rats (Maseko et al . ). In contrast, the permeability of the mucosa to FD4 was reduced by 53% in heat‐stressed pigs when Se plus VE was increased from the lowest to the highest dose, and by 44% in pigs kept in thermoneutral conditions.…”

Transcriptome Analysis Reveals Potential Regulatory Genes Related to Heat Tolerance in Holstein Dairy Cattle

“…The expression of BPI in fish was significantly up-regulated post crowding stress exposure [51]. Heat stress increased the expression of GPX2 (2.23-fold change) relative to thermo-neutral conditions in rat ileum [52]. Heat stress improved the EGF mRNA expression, and the production of EGF or EGF-like peptides under heat stress may be an important survival mechanism in mouse mammary epithelial cells [53].…”

Supplementation of cultivated mushroom species with selenium: bioaccumulation and speciation study

“…The total content of Se in mushrooms was found to vary from <0.5 mg kg −1 DW (Cantharellus cibarius or Xerocomus badius) to >20 mg kg −1 DW (Boletus edulis). Unfortunately, wild edible mushrooms grow seasonally only; therefore, an alternative is to supplement Se using cultivated mushrooms, especially those which are economically important such as species of Pleurotus, Pholiota or Agaricus genera [19][20][21]. It should be highlighted that in many countries and regions, the consumption of mushrooms constitutes an important and integral part of the human diet [22].…”