Selenium-Enriched Lactobacillus acidophilus Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice by Regulating Inflammatory Cytokines and Intestinal Microbiota

Abstract

Aim: To evaluate the effect of Selenium-enriched Lactobacillus acidophilus (Se-enriched L. acidophilus) on dextran sulfate sodium (DSS)-induced colitis in mice.Methods: Mice were randomly divided into four groups: a control group, a control + Se-enriched L. acidophilus group, a chronic colitis group, and a chronic colitis + Se-enriched L. acidophilus group (n = 10 each group). The mice were sacrificed on the 26th day. The disease activity index, survival rates, and histological injury score were determined. Cytokines produced by lamina propria lymphocytes (LPLs), the selenium (Se) concentrations in serum and colon tissue and the mouse intestinal microbiota were evaluated.Results: Se-enriched L. acidophilus can improve histological injury and the disease activity index in mice with chronic colitis and reduce IL-1β, IL-6, IL-12p70, TNF-α, IL-23, IFN-γ, IL-17A, and IL-21 (P < 0.05) and increase IL-10 (P < 0.05) expression levels. Moreover, Se-enriched L. acidophilus can increase the β diversity of intestinal microbiota in mice with chronic colitis, significantly reduce the relative abundance of Lactobacillus and Romboutsia (P < 0.05), and significantly increase the relative abundance of Parasutterella (P < 0.05).Conclusions: Se-enriched L. acidophilus can improve DSS-induced chronic colitis by regulating inflammatory cytokines and intestinal microbiota.

Cepharanthine ameliorates dextran sulphate sodium‐induced colitis through modulating gut microbiota

“…The relative abundance of Romoutsia also increased in DSS‐induced colitis in the study of Wu et al . (2021), which is consistent with our findings.…”

“…A study has shown that the relative abundance of Romboutsia is correlated with circulating inflammatory (IL-1b) and anxiety-like behaviour (Grant et al, 2021). The relative abundance of Romoutsia also increased in DSS-induced colitis in the study of Wu et al (2021), which is consistent with our findings.…”

Lactiplantibacillus plantarum J‐15 reduced calcium oxalate kidney stones by regulating intestinal microbiota, metabolism, and inflammation in rats

“…As a probiotic, J‐15 mainly plays a role in regulating intestinal flora affected by kidney stone formation and helped restore the intestinal flora. This is pretty consistent with previous investigation on the changes in intestinal or fecal abundance of genus Lactobacillus after the administration of Lactobacillus strains in other disease models, 52,53 which might be due to the strain specificity, dosage, different animal models, and intestinal segments 54–56 . Our previous study on the microbial community composition of different intestinal segments after L .…”

“…This is pretty consistent with previous investigation on the changes in intestinal or fecal abundance of genus Lactobacillus after the administration of Lactobacillus strains in other disease models, 52,53 which might be due to the strain specificity, dosage, different animal models, and intestinal segments. [54][55][56] Our previous study on the microbial community composition of different intestinal segments after L. plantarum administration revealed that the amount of genus Lactobacillus varied widely among intestinal segments, 57 with the higher levels in the ileum and less in the cecum and colon. It suggests that L. plantarum significantly regulates the intestinal microflora of the ileum, rather than the cecum and colon.…”

Comparison of Selenium-Enriched Lactobacillusparacasei, Selenium-Enriched Yeast, and Selenite for the Alleviation of DSS-Induced Colitis in Mice

“…As only one type of organic Se supplement is currently available, it is necessary to develop new organic Se products. Previous studies have shown that probiotics can be introduced to medium containing inorganic Se using the biotransformation method, which can convert inorganic Se into organic Se and form Se-enriched probiotics [14]. SeMet and SeCys are the main forms of Se in Se-enriched products.…”