Selenium-Substituted Hydroxyapatite Nanoparticles and their in Vitro Interaction on Human Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells

Korowash, Sara Ibrahim; Burdzińska, Anna; Pędzisz, Piotr; Dąbrowski, Filip; Mostafa, Amany A.; Abdel-Razik, A.; Mahgoub, A. E.; Ibrahim, D. M.

doi:10.1007/bf03401219

Cited by 3 publications

(9 citation statements)

References 35 publications

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“…) in hydroxyapatite. 14,41,44 The FESEM images obtained at the two magnifications (1.00 and 10.00 KX) revealed that the hydroxyapatite powders are made up of clusters of tiny particles that appear to be spherical in shape (Figure 3). This agglomeration might be connected to the preparation's 24-h stirring period.…”

Section: Resultsmentioning

confidence: 99%

“…All these elements have a vital role in the cell growth and proliferation of the cells as well as the antibacterial activity effect. [10][11][12][13][14]18,21,53,54 The occurrence of S 2ion in the hydroxyapatite was detected by ICP-OES in copper containing HA powders samples accompanying substitution of Cu 2+ in the lattice indicated the dual substitution might have been caused by CuSO 4 .5H 2 O, the precursor used.…”

Section: Resultsmentioning

confidence: 99%

“…These bands were attributed to the vibrations of Se–O bands of the selenite ion (SeO 3 2- ) in hydroxyapatite. 14,41,44

Figure 2.FTIR spectra of different hydroxyapatite powders: HA, SeHA, CuHA and SeCuHA.…”

Section: Resultsmentioning

confidence: 99%

“…12 Selenium has been shown to help with cardiovascular disease, cancer, thyroid, brain, bone, tissue reproduction, viral infections, and the immune system. 13 A series of different concentrations of selenium (between 1 to 5 mol %) substituted hydroxyapatite powders were prepared previously by Korowash et al (2017) using an aqueous precipitation method. In their study, the cytotoxicity of the powders on both human bone marrow mesenchymal stem cells (BM-MSCs) and umbilical cord-derived mesenchymal stem cells (UC-MSCs) was studied in vitro and in their work in comparison to pure HA powders, 0.592 mM Se, corresponding to a 2 mol% Se showed no cytotoxicity, but stimulated proliferation of UC-MSCs.…”

Section: Introductionmentioning

confidence: 99%

“…In their study, the cytotoxicity of the powders on both human bone marrow mesenchymal stem cells (BM-MSCs) and umbilical cord-derived mesenchymal stem cells (UC-MSCs) was studied in vitro and in their work in comparison to pure HA powders, 0.592 mM Se, corresponding to a 2 mol% Se showed no cytotoxicity, but stimulated proliferation of UC-MSCs. 14 The poor antibacterial activity of pure HA limits its longterm stability and increases the risk of implant-related infections and the likelihood of implantation failures. 15 Therefore, researchers have been exploring different approaches to enhance the antibacterial properties of HA to overcome this challenge.…”

Section: Introductionmentioning

confidence: 99%

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Selenium- and/or copper-substituted hydroxyapatite: A bioceramic substrate for biomedical applications

Korowash,

Keskin-Erdogan,

Hemdan

et al. 2023

J Biomater Appl

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Atomic substitution or doping of a bioceramic material hydroxyapatite (HA) with specific ions is an appealing approach for improving its biocompatibility and activity, as well as imparting antibacterial properties. In this study, selenium- and/or copper-substituted hydroxyapatite powders were synthesized by an aqueous precipitation method and using the freeze-drying technique. The molar concentrations of constituents were calculated based on the proposed mechanism whereby selenium (Se4+) ions partially substitute phosphorus (P5+) sites, and copper (Cu2+) ions partially substitute (Ca2+) sites in the HA lattice. Dried precipitated samples were characterized using Inductively coupled plasma optical emission spectroscopy (ICP-OES), X-ray diffraction analysis (XRD), Fourier-transform infrared spectroscopy (FTIR) and Field-emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FESEM-EDX). Accordingly, substitution of Se4+ and/or Cu2+ ions took place in the crystal lattice of HA without the formation of any impurities. The presence of sulphur (S2-) ions in the hydroxyapatite was detected by ICP-OES in all samples with copper substituted in the lattice. The cytotoxicity of the powders on osteoblastic (MC3T3-E1) cells was evaluated in vitro. Selenium substituted hydroxyapatite (SeHA), at the concentration (200 μg/mL), demonstrated higher populations of the live cells than that of control (cells without powders), suggesting that selenium may stimulate the proliferation of these cells. In addition, the copper substituted hydroxyapatite (CuHA) and the selenium and copper substituted hydroxyapatite (SeCuHA) at the concentrations (200 and 300 μg/mL) and (200 μg/mL), respectively demonstrated better results than the unsubstituted HA. Antimicrobial activity was assessed using a well-diffusion method against Streptococcus mutans and Candida albicans, and superior results has obtained with SeCuHA samples. Presented findings imply that selenium and/or copper substituted modified hydroxyapatite nanoparticles, may be an attractive antimicrobial and cytocompatible substrate to be considered for use in a range of translational applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…These bands were attributed to the vibrations of Se–O bands of the selenite ion (SeO 3 2- ) in hydroxyapatite. 14,41,44

Figure 2.FTIR spectra of different hydroxyapatite powders: HA, SeHA, CuHA and SeCuHA.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Selenium- and/or copper-substituted hydroxyapatite: A bioceramic substrate for biomedical applications

Korowash,

Keskin-Erdogan,

Hemdan

et al. 2023

J Biomater Appl

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Comparative study of certain substituted ions in hydroxyapatite: characterization and effect on cell viability

Korowash¹,

Abo-Almaged²,

Ibrahim³

2022

Adv. Nat. Sci: Nanosci. Nanotechnol.

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Hydroxyapatite (HA) is a biomaterial that has been widely studied for uses such as bone grafting and tissue engineering. Pure and hydroxyapatite nanoparticles with cationic (Na, Ti, and Li) as well as anionic ( CO 3 2 − and Si4+) substitutions were synthesised using a wet chemical precipitation method followed by freeze-drying and investigated for their cytotoxicity effect. Inductively coupled plasma (ICP), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy were used to characterise the produced powders. The production of the HA phase was confirmed in all synthesised HA samples. MTT (3-(4.5-dimethylthiazol-2-yl)−2.5-diphenyl tetrazolium bromide) assay was performed using the BJ1 cell line of human normal fibroblasts to investigate the cytotoxicity of the synthesised HA samples. The cytotoxicity test showed that the HA samples were biocompatible, with the greatest results coming from Li-substituted (LiHA2) and Na-substituted (NaHA) hydroxyapatite.

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Novel selenium and/or copper substituted hydroxyapatite–gelatin–chitosan–eggshell membrane nanocomposite scaffolds for bone tissue engineering applications

Korowash,

Sharifulden,

Ibrahim

et al. 2023

Journal of Applied Biomaterials & Functional Materials

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Limitations with the majority of bone therapeutic treatments include low availability, ethical constraints and low biological compatibility. Although a number of choice materials have been exploited successfully, there has always been scope for improvement as well as development of the next-generation of materials. Herein, scaffolds – developed from gelatin, chitosan and eggshell membranes – were crosslinked using tannic acid, and further infused with selenium and/or copper substituted hydroxyapatite nanoparticles to generate a novel nanocomposite substrate. FESEM images of the nanocomposite scaffolds revealed the presence of interconnected pores, mostly spread over the whole surface of the scaffold, alongside XRD and FTIR profiling that detailed the formation of hydroxyapatite as a sole phase. Moreover, physical characterisation of the nanocomposite confirmed that the hydroxyapatite particulates and the eggshell membrane fibres were uniformly distributed and contributed to the surface roughness of the scaffold. Biocompatibility and cytotoxicity of the novel constructs were assessed using the mouse-derived osteoblastic cell line, MC3T3-E1, and standard cell culture assays. Metabolic activity assessment (i.e. MTS assay), LDH-release profiles and Live/Dead staining demonstrated good cell adhesion, viability, and proliferation rates. Accordingly, this work summarises the successful development of a novel construct which may be exploited as a clinical/therapeutic treatment for bone repair as well as a possible translational application as a novel biomaterial for the drug development pipeline.

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Selenium-Substituted Hydroxyapatite Nanoparticles and their in Vitro Interaction on Human Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells

Cited by 3 publications

References 35 publications

Selenium- and/or copper-substituted hydroxyapatite: A bioceramic substrate for biomedical applications

Selenium- and/or copper-substituted hydroxyapatite: A bioceramic substrate for biomedical applications

Comparative study of certain substituted ions in hydroxyapatite: characterization and effect on cell viability

Novel selenium and/or copper substituted hydroxyapatite–gelatin–chitosan–eggshell membrane nanocomposite scaffolds for bone tissue engineering applications

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