Selenomethionine modulates insulin secretion in the MIN6‐K8 mouse insulinoma cell line

Abstract

Selenium is an essential trace element of interest for its potential role in glucose homeostasis. The present study investigated the impact of selenium supplementation as selenomethionine (SeMet) on insulin secretion in MIN6‐K8 cells, a pancreatic β‐cell model. We found that SeMet enhanced percent glucose‐induced insulin secretion, while also increasing tolbutamide‐ and KCl‐induced percent insulin secretion. RNA‐sequencing showed that SeMet supplementation altered expression of several selenoproteins, including glutathione peroxidase 3 (Gpx3) and selenoprotein P (SelP). Targeted knockdown of Gpx3 increased both percent and total insulin release, while SelP knockdown increased insulin content and insulin release. Collectively, these studies support a putative role for selenium and selenoproteins in the regulation of insulin secretion, glucose homeostasis, and diabetes risk.

Administration of selenomethionine in combination with serine benefits diabetes via gut microbiota

“…Supplementation with sodium selenite exerts insulin-like effects, and a high-dose of sodium selenite administrated acutely results in hypoglycemia ( Becker et al, 1994 ; Stapleton, 2000 ). Importantly, selenomethionine, as an organic selenium source, was proved to involve in insulin secretion and glucose homeostasis ( Zhao et al, 2021 ). However, no results have reported the in vivo beneficial effects of selenomethionine on diabetic individuals.…”

Experimental Approaches to Diabetes Mellitus

“…Islet isolation for in vitro insulin secretion is costly and difficult, which has led to the use of insulinoma cells for this purpose. 33 - 36 Easy reproduction in culture and the ability to form secretion with glucose stimulation are its most important advantages. 37 - 39 …”