Selenoneine and ergothioneine in human blood cells determined simultaneously by HPLC/ICP-QQQ-MS

Kroepfl, Nina; Francesconi, Kevin A.; Schwerdtle, Tanja; Kuehnelt, Doris

doi:10.1039/c8ja00276b

Cited by 22 publications

(15 citation statements)

References 30 publications

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“…Besides this unknown species, a peak that was not baseline resolved from selenoneine was detected at its high retention time side in HPLC–ICPMS (Figure ), hinting toward the formation of another metabolite specific for selenoneine. HPLC–HR‐ESI–MS confirmed the presence of Se‐methylselenoneine ( Figure ), a metabolite of selenoneine recently discovered in human blood and urine . Besides being present in the cell lysates, Se‐methylselenoneine could not be unambiguously identified in the culture dish media after 5 min or 8 h of incubation with selenoneine, but was clearly detectable after 72 h of treatment (Figure S2, Supporting Information), confirming its formation from selenoneine.…”

Section: Resultsmentioning

confidence: 59%

“…In media and cell lysates obtained from the Transwell experiments, selenoneine accounted for ≈80–110% of the total Se in the media and 55–90% in the cell lysates ( Table ). To investigate a potential underestimation of the concentration of selenoneine due to its binding to thiol groups, 0.1 m m tris(2‐carboxyethyl)phosphine (TCEP) was added to the RP mobile phase (chromatographic condition I, Table 1, Supporting Information) as a reducing agent, as previously tested . Results obtained with TCEP in the mobile phase did, however, not differ significantly from the results obtained without it, indicating that selenoneine was not bound to other matrix‐components to a larger extent (data not shown).…”

Section: Resultsmentioning

confidence: 92%

“…After its first identification in tuna, where it constitutes the major water‐extractable Se species in muscle and blood, significant amounts of selenoneine were found in other marine fish species, and smaller quantities in terrestrial animals . Moreover, selenoneine as well as its methylated metabolite Se‐methylselenoneine have been detected in human blood . Consequently, selenoneine is bioavailable to humans and seems to undergo metabolic transformations.…”

Section: Introductionmentioning

confidence: 99%

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Side‐Directed Transfer and Presystemic Metabolism of Selenoneine in a Human Intestinal Barrier Model

Rohn

Kroepfl

Bornhorst

et al. 2019

Molecular Nutrition Food Res

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Scope: Selenoneine, a recently discovered selenium (Se) species mainly present in marine fish, is the Se analogue of ergothioneine, a sulfur-containing purported antioxidant. Although similar properties have been proposed for selenoneine, data on its relevance to human health are yet scarce. Here, the transfer and presystemic metabolism of selenoneine in an in vitro model of the human intestinal barrier are investigated. Methods and results: Selenoneine and the reference species Se-methylselenocysteine (MeSeCys) and selenite are applied to the Caco-2 intestinal barrier model. Selenoneine is transferred in higher amounts, but with similar kinetics as selenite, while MeSeCys shows the highest permeability. In contrast to the reference species, transfer of selenoneine is directed toward the blood side. Cellular Se contents demonstrate that selenoneine is efficiently taken up by Caco-2 cells. Moreover, HPLC/MS-based Se speciation studies reveal a partial metabolism to Se-methylselenoneine, a metabolite previously detected in human blood and urine. Conclusions: Selenoneine is likely to pass the intestinal barrier via transcellular, carrier-mediated transport, is highly bioavailable to Caco-2 cells and undergoes metabolic transformations. Therefore, further studies are needed to elucidate its possible health effects and to characterize the metabolism of selenoneine in humans.

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Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Side‐Directed Transfer and Presystemic Metabolism of Selenoneine in a Human Intestinal Barrier Model

Rohn

Kroepfl

Bornhorst

et al. 2019

Molecular Nutrition Food Res

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“…Leaving aside early efforts such as the colorimetric methods of Hunter (365) , of Melville and colleagues (76,85,366) and of Carlsson et al (367) , a variety of analytical methods have been proposed (4) , mostly involving capillary electrophoresis (368,369) or chromatography (368,(370)(371)(372) coupled to absorbance (373,374) , fluorescence detection (375)(376)(377)(378) , electrochemical detection (379) or MS (72,127,256,368,378,(380)(381)(382) . A useful feature is that ERG is unusually stable, in that anhydrous ERG decomposes only at 275-276°C (76) , allowing its isolation at temperatures close to that of boiling water (72) .…”

Section: Analyticsmentioning

confidence: 99%

The biology of ergothioneine, an antioxidant nutraceutical

et al. 2020

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Ergothioneine (ERG) is an unusual thio-histidine betaine amino acid that has potent antioxidant activities. It is synthesised by a variety of microbes, especially fungi (including in mushroom fruiting bodies) and actinobacteria, but is not synthesised by plants and animals who acquire it via the soil and their diet, respectively. Animals have evolved a highly selective transporter for it, known as solute carrier family 22, member 4 (SLC22A4) in humans, signifying its importance, and ERG may even have the status of a vitamin. ERG accumulates differentially in various tissues, according to their expression of SLC22A4, favouring those such as erythrocytes that may be subject to oxidative stress. Mushroom or ERG consumption seems to provide significant prevention against oxidative stress in a large variety of systems. ERG seems to have strong cytoprotective status, and its concentration is lowered in a number of chronic inflammatory diseases. It has been passed as safe by regulatory agencies, and may have value as a nutraceutical and antioxidant more generally.

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“…73 Furthermore, ICP-QMS/QMS has been covered by principle studies and reviews on the ICP-MS reaction/collision cell technology, 72,74,75 gas chromatography (GC-) ICP-MS, 76 and high performance liquid chromatography (HPLC-) ICP-MS for quantitative profiling analysis of metalloids in biological samples, 77 respectively. ion-gas molecule reaction, 73 isotope, 3,4,22-24,26-28,43-47,62-66,68, single particle, 5,6,15,31,40,[54][55][56][57] and chemical speciation, 7,8,[10][11][12]16,76,77 respectively.…”

Section: References Investigatedmentioning

confidence: 99%

Trends and Advances in Inductively Coupled Plasma Tandem Quadruple Mass Spectrometry (ICP-QMS/QMS) With Reaction Cell

Zhu¹

2021

At.Spectrosc.

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Trends and advances in the development and application of inductively coupled plasma tandem quadrupole mass spectrometry (ICP-QMS/QMS) with a reaction cell is reviewed mainly based on publications from January 2018 to July 2021. ICP-QMS/QMS has been applied in various research fields covering the sciences of biology, energy, environmental, food/medical, geology, materials, and radionuclide. The objectives of analysis cover the determination of elemental concentration, ion-gas reaction, isotope analysis, single particle analysis, and chemical speciation analysis. Measurement of most elements in the periodic table are reported except for H, N, O, F, rare gas, and some of the radionuclides. In addition to the default reaction/collision gases (i.e., He, H2, O2, and NH3), N2O, CO2, CH4, CH3F, C2H4, and C2H6 have been used as reaction gases to improve the capability of separating spectral interferences or to study the ion-molecule reactions. Typical applications of ICP-QMS/QMS analysis in the major research fields are also discussed.

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Selenoneine and ergothioneine in human blood cells determined simultaneously by HPLC/ICP-QQQ-MS

Abstract: An HPLC/ICP-QQQ-MS method for the simultaneous quantitative determination of the health relevant anti-oxidant ergothioneine and its selenium-analogue selenoneine in blood cells is presented.

Cited by 22 publications

References 30 publications

Side‐Directed Transfer and Presystemic Metabolism of Selenoneine in a Human Intestinal Barrier Model

Side‐Directed Transfer and Presystemic Metabolism of Selenoneine in a Human Intestinal Barrier Model

The biology of ergothioneine, an antioxidant nutraceutical

Trends and Advances in Inductively Coupled Plasma Tandem Quadruple Mass Spectrometry (ICP-QMS/QMS) With Reaction Cell

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