Selenoprotein K enhances STING oligomerization to facilitate antiviral response

Lv, Lin; Chai, Li; Wang, Jie; Wang, Mengge; Qin, Danhui; Song, Hui; Fu, Yue; Zhao, Chenyang; Jia, Jihui; Zhao, Wei; Jia, Mutian

doi:10.1371/journal.ppat.1011314

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…These findings suggest that adequate SePCH can help maintain ER redox homeostasis by increasing the levels of SEP15, SEPT2 and SEPH in largemouth bass ( 60 ). Furthermore, SEPK, an endoplasmic reticulum-membrane protein, plays a crucial role in facilitating effective Ca 2+ flux during immune cell activation induced by dietary Se ( 61 ). In our study, SEPK expression levels were enhanced in the 0.60 and 1.20 g/Kg SePCH groups, consistent with findings in Nile tilapia ( 47 ), rainbow trout ( 58 ), yellow catfish ( 59 ), grass carp ( 62 ) and pig ( 63 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of selenoprotein extracts from Cardamine hupingshanensis on growth, selenium metabolism, antioxidant capacity, immunity and intestinal health in largemouth bass Micropterus salmoides

Zhang,

Zhao,

Zhang

et al. 2024

Front. Immunol.

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This study aimed to assess the impact of dietary selenoprotein extracts from Cardamine hupingshanensis (SePCH) on the growth, hematological parameters, selenium metabolism, immune responses, antioxidant capacities, inflammatory reactions and intestinal barrier functions in juvenile largemouth bass (Micropterus salmoides). The base diet was supplemented with four different concentrations of SePCH: 0.00, 0.30, 0.60 and 1.20 g/Kg (actual selenium contents: 0.37, 0.59, 0.84 and 1.30 mg/kg). These concentrations were used to formulate four isonitrogenous and isoenergetic diets for juvenile largemouth bass during a 60-day culture period. Adequate dietary SePCH (0.60 and 1.20 g/Kg) significantly increased weight gain and daily growth rate compared to the control groups (0.00 g/Kg). Furthermore, 0.60 and 1.20 g/Kg SePCH significantly enhanced amounts of white blood cells, red blood cells, platelets, lymphocytes and monocytes, and levels of hemoglobin, mean corpuscular volume and mean corpuscular hemoglobin in the hemocytes. In addition, 0.60 and 1.20 g/Kg SePCH increased the mRNA expression levels of selenocysteine lyase, selenophosphate synthase 1, 15 kDa selenoprotein, selenoprotein T2, selenoprotein H, selenoprotein P and selenoprotein K in the fish liver and intestine compared to the controls. Adequate SePCH not only significantly elevated the activities of antioxidant enzymes (Total superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase), the levels of total antioxidant capacity and glutathione, while increased mRNA transcription levels of NF-E2-related factor 2, Cu/Zn-superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase. However, adequate SePCH significantly decreased levels of malondialdehyde and H2O2 and the mRNA expression levels of kelch-like ECH-associated protein 1a and kelch-like ECH-associated protein 1b in the fish liver and intestine compared to the controls. Meanwhile, adequate SePCH markedly enhanced the levels of immune factors (alkaline phosphatase, acid phosphatase, lysozyme, complement component 3, complement component 4 and immunoglobulin M) and innate immune-related genes (lysozyme, hepcidin, liver-expressed antimicrobial peptide 2, complement component 3 and complement component 4) in the fish liver and intestine compared to the controls. Adequate SePCH reduced the levels of pro-inflammatory cytokines (tumour necrosis factor-α, interleukin 8, interleukin 1β and interferon γ), while increasing transforming growth factor β1 levels at both transcriptional and protein levels in the liver and intestine. The mRNA expression levels of mitogen-activated protein kinase 13 (MAPK 13), MAPK14 and nuclear factor kappa B p65 were significantly reduced in the liver and intestine of fish fed with 0.60 and 1.20 g/Kg SePCH compared to the controls. Histological sections also demonstrated that 0.60 and 1.20 g/Kg SePCH significantly increased intestinal villus height and villus width compared to the controls. Furthermore, the mRNA expression levels of tight junction proteins (zonula occludens-1, zonula occludens-3, Claudin-1, Claudin-3, Claudin-5, Claudin-11, Claudin-23 and Claudin-34) and Mucin-17 were significantly upregulated in the intestinal epithelial cells of 0.60 and 1.20 g/Kg SePCH groups compared to the controls. In conclusion, these results found that 0.60 and 1.20 g/Kg dietary SePCH can not only improve growth, hematological parameters, selenium metabolism, antioxidant capacities, enhance immune responses and intestinal functions, but also alleviate inflammatory responses. This information can serve as a useful reference for formulating feeds for largemouth bass.

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Section: Discussionmentioning

confidence: 99%

Effects of selenoprotein extracts from Cardamine hupingshanensis on growth, selenium metabolism, antioxidant capacity, immunity and intestinal health in largemouth bass Micropterus salmoides

Zhang,

Zhao,

Zhang

et al. 2024

Front. Immunol.

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show abstract

“…Immune regulation, a critical biological function of Se, engages various selenoproteins [ 63 , 64 ], of which SELENOK is a key immune regulator [ 35 , 37 ]. SELENOK deficiency hinders the proliferation, differentiation, and migration of T cells and neutrophils and is crucial for macrophage activation and phagocytosis [ 35 , 65 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

“…SELENOK, localized to the ER membrane and highly expressed in immune cells, is required for the immune response, proliferation, and differentiation of various immune cells [ [35] , [36] , [37] ]. Recent in vitro research has shown that SELENOK overexpression markedly augments microglial migration and phagocytosis, indicating a probable impact on central nervous system immunity through microglial modulation [ 38 ].…”

Section: Introductionmentioning

confidence: 99%