Aptamers in Bioanalysis 2008
DOI: 10.1002/9780470380772.ch2
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Selex and Its Recent Optimizations

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Cited by 12 publications
(10 citation statements)
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“…The selection process consists of five steps: 1) binding, incubation of the library with the target; 2) partition, isolation of target-bound sequences from unbound ones; 3) elution from a complex via chromatography; 4) amplification, generation of a new pool of nucleic acids by PCR (for DNA libraries) or RT-PCR (for RNA libraries); and 5) conditioning, in which in vitro transcription and purification of relevant ssDNA are included. 18 …”
Section: Aptamer Selectionmentioning
confidence: 99%
“…The selection process consists of five steps: 1) binding, incubation of the library with the target; 2) partition, isolation of target-bound sequences from unbound ones; 3) elution from a complex via chromatography; 4) amplification, generation of a new pool of nucleic acids by PCR (for DNA libraries) or RT-PCR (for RNA libraries); and 5) conditioning, in which in vitro transcription and purification of relevant ssDNA are included. 18 …”
Section: Aptamer Selectionmentioning
confidence: 99%
“…There have been an incredible number of optimizations to the SELEX protocol in recent years [42] , and nearly all of them are applicable to the Genomic SELEX method. However, since Genomic SELEX drastically decreases the diversity of the initial library, many of these biases will not play such a critical role in selectivity.…”
Section: Summary/conclusionmentioning
confidence: 99%
“…An in vitro process, termed Systematic Evolution of Ligands by Exponential Enrichment (SELEX), is commonly used to select and synthesize, with high reproducibility, purity and in large quantity, the required aptamers. Moreover, this technique seems able to generate aptamers for virtually each possible target [2], overcoming the needs for cell lines or animals [1]. To date, numerous high-affinity aptamers have been selected for a broad range of target molecules, including metal ions, peptides, drugs, proteins and even whole cells or viruses [1,3,4].…”
Section: Introductionmentioning
confidence: 99%