Self- and Cross-incompatibility of Various Citrus Accessions

Yamamoto, Masashi; Kubo, Tatsuya; Tominaga, Shigeto

doi:10.2503/jjshs.75.372

Cited by 61 publications

(51 citation statements)

References 7 publications

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“…Generative cell division occurs by three days after pollination in compatible tubes, but only rarely in incompatible tubes in Citrus (Kahn and DeMason, 1988) and almost all own pollen tubes were arrested in the styles (Wakana et al, 2004;Yamamoto et al, 2006). In pistils of self-incompatible Citrus plants pollinated with their own pollen, no pollen tubes reached the ovaries (Kahn and DeMason, 1988;Ton and Krezdorn, 1967).…”

Section: Introductionmentioning

confidence: 99%

Determination of Self-incompatible Citrus Cultivars with S1 and/or S2 Alleles by Pollination with Homozygous S1 Seedlings (S1S1 or S2S2) of ‘Banpeiyu’ Pummelo

Kim

Mori

Wakana

et al. 2011

J. Japan. Soc. Hort. Sci.

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Gametophytic self-incompatibility (SI), an important character for breeding seedless cultivars of Citrus, is known in pummelo, mandarin, and many hybrid cultivars with pummelo in their pedigrees. Only a little is known about the allelic variation in the self-incompatibility gene (S), S allele frequencies, and the genotypes of self-incompatible and semi-self-compatible cultivars. In this study, pollination of 'Banpeiyu' S 1 seedlings with 'Banpeiyu' and pollination between the S 1 seedlings were performed to determine homozygous S 1 seedlings for the S gene. Seventyeight Citrus accessions, including 55 pummelo accessions, were pollinated with each of two homozygous S 1 seedlings (S 1 S 1 and S 2 S 2 ). Pollen tube arrest in the style base of their pollinated pistils indicated that 23 accessions, including 'Banpeiyu', have an S 1 allele each and 16 accessions, including 'Banpeiyu', have an S 2 allele each. Frequency of accessions with S 1 allele was 29.9% (23 of 77 accessions examined) and S 1 allele frequency was 16.4% (23 of 140 alleles excluding S f allele). Frequency of accessions with S 2 allele was 21.3% (16 of 75 accessions examined) and S 2 allele frequency was 11.6% (16 of 138 alleles excluding S f allele). Pummelo accessions collected from Kagoshima Prefecture had S 1 alleles with two and half times higher frequency (56.3%) than that in all accessions examined. Of the 79 accessions, six accessions ('Banpeiyu', 'Iriki Buntan', 'Kaopang', Nagashima Buntan No. 6, Nagashima Buntan No. 7, and 'Soyu') were S 1 S 2 genotypes. The Citrus cultivars, whose S genotypes have been fully determined in this study, were

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Section: Introductionmentioning

confidence: 99%

Determination of Self-incompatible Citrus Cultivars with S1 and/or S2 Alleles by Pollination with Homozygous S1 Seedlings (S1S1 or S2S2) of ‘Banpeiyu’ Pummelo

Kim

Mori

Wakana

et al. 2011

J. Japan. Soc. Hort. Sci.

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“…The CAPS genotypes of #16 and 23 may have arisen from self-fertilized seedlings of sour orange. This is because sour orange is selfcompatible (Yamamoto et al, 2006) and seedling propagation has been common there.…”

Section: Discussionmentioning

confidence: 99%

Investigation and DNA Analysis of Local <i>Citrus</i> Genetic Resources Grown on the Chuuk Islands of Micronesia

2018

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An investigation of local citrus genetic resources grown on the Chuuk Islands of the Federated States ofMicronesia was conducted in August 2013. A total of 21 accessions were examined. According to the morphological traits, ten, four, four, two, and one accession resembled lime (local name "Nayimis"), sour orange ("Kurukur"), calamondin ("Tangerin" and "Gunkan"), sweet orange ("Kurukur" and "American Kurukur"), and pummelo ("Kurukur"), respectively. Next, cleaved amplified polymorphic sequence (CAPS) analysis was conducted. Then, precise accession identification was made based on the results of CAPS analysis in conjunction with morphological traits. "Nayimis" accessions were classified into at least two types. Genotypes of "Nayimis" type 1 were identical to those of Mexican lime. A genetic influence from mandarin was detected in "Nayimis" type 2. Although the morphological traits of all "Kurukur" sour orange types were similar, their diversity was revealed by CAPS analysis. The genotype of one "Kurukur" accession was identical to that of sour orange. Genotypes of the sweet orange-type "Kurukur" and "American Kurukur" were different. The types of "American Kurukur" and sweet orange were the same. Pummelo-type "Kurukur" was considered to be true pummelo based on the results of morphological and molecular markers. Morphologically, the calamondin-like accessions "Tangerin" and "Gunkan" showed the same genotype combinations on CAPS analysis. These genotypes were identical to those of calamondin used as a control. The combined results of morphological and molecular markers offer valuable information for the identification of citrus genetic resources on the Chuuk Islands.

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“…These trees were grown in the orchard of Kyushu University, Fukuoka, Japan. Grapefruit and sweet orange are semi self-compatible (Soost, 1969;Yamamoto et al, 2006) and polyembryonic. Selfincompatibility and monoembryony in other cultivars and accessions are listed in Table 1.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Soost (1969) also found that the genotype of self-compatible grapefruit was composed of self-fertility (S f ) and self-incompatibility (S n ) alleles, since F 1 seedlings obtained by several crosses with grapefruit segregated into self-compatible and self-incompatible seedlings. Vardi et al (2000) and Yamamoto et al (2006) also found on the basis of segregation of selfincompatible hybrid seedlings that the genotypes of satsuma mandarin and sweet orange were S f S n . For a considerably long time after the work of Soost (1969), there was nothing but a few reports on the genotyping of Citrus cultivars for the S gene (Kim et al, 2010;Ngo et al, 2010b;Wakana et al, 1998), probably because of the existence of several barriers, such as male and/or female sterility, nucellar embryony, and a very long juvenile phase in zygotic seedlings for five to more than ten years.…”

Section: Introductionmentioning

confidence: 99%

“…Because of the economic importance of both pollination for seeded fruit production in Citrus cultivars without high parthenocarpy and seedless fruit production in those with high parthenocarpy, many studies of selfincompatibility have been carried out (Hearn, 1969;Iwamasa and Oba, 1980;Soost, 1964;Yamada, 1988;Yamamoto et al, 2006), since Nagai and Tanikawa (1928) firstly reported that several Citrus varieties were self-incompatible. Soost (1965Soost ( , 1969 studied the segregation of self-incompatibility in F 1 progenies from several crosses with Citrus cultivars and found a gametophytic self-incompatibility system, which may be controlled by a single codominant S gene with multiple S alleles.…”

Section: Introductionmentioning

confidence: 99%

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Estimation of Self-incompatibility Genotypes of Citrus Cultivars with Got-3 Allozyme Markers

Ngo

Kim

Wakana

et al. 2011

J. Japan. Soc. Hort. Sci.

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Self-incompatibility (SI) in Citrus is known in cultivars and plants of pummelo, pummelo relative, and mandarin; however, the detailed mechanism of self-incompatibility and the allelic diversity of the SI gene (S) are not known because of the presence of barriers such as nucellar embryony, male sterility, and a long juvenile phase. Thus, there is little information on S genotypes of Citrus cultivars at present. In this study, the S genotypes of Citrus cultivars were estimated with the aid of allozymes produced by a glutamate oxaloacetate transaminase isozyme gene (Got-3), which appeared to link to the S gene. Of twenty-two F 1 progenies from eleven crosses and their reciprocal crosses with eight monoembryonic SI cultivars, including 'Banpeiyu' pummelo, three F 1 progenies from three crosses and three from reciprocal crosses showed segregation distortion for the Got-3 gene, suggesting the presence of the same S alleles in the cultivars used for the reciprocal crosses. Defining the 'Banpeiyu' genotype as S 1 S 2 , the genotype was estimated as S 1 S 3 for 'Tosa Buntan', S 4 S 5 for 'Hassaku', S 6 S 7 for 'Yuge-hyokan', and S 1 S 6 for 'Shishiyuzu'. Further allozyme analyses for segregation distortion in progenies from eight crosses with eight monoembryonic SI cultivars suggested the possibility that 'Hyuganatsu' has S 1 allele, while those from 15 crosses between the eight monoembryonic SI cultivars and five polyembryonic self-compatible cultivars suggested the possibility that 'Rough lemon' has S 1 allele. The estimated genotypes, consisting of two of the nine alleles (S 1 to S 8 and S f ) in these Citrus cultivars, may be useful for further estimation and determination of S genotypes in Citrus cultivars and plants.

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Self- and Cross-incompatibility of Various Citrus Accessions

Cited by 61 publications

References 7 publications

Determination of Self-incompatible Citrus Cultivars with S1 and/or S2 Alleles by Pollination with Homozygous S1 Seedlings (S1S1 or S2S2) of ‘Banpeiyu’ Pummelo

Determination of Self-incompatible Citrus Cultivars with S1 and/or S2 Alleles by Pollination with Homozygous S1 Seedlings (S1S1 or S2S2) of ‘Banpeiyu’ Pummelo

Investigation and DNA Analysis of Local <i>Citrus</i> Genetic Resources Grown on the Chuuk Islands of Micronesia

Estimation of Self-incompatibility Genotypes of Citrus Cultivars with Got-3 Allozyme Markers

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