Self‐assembled biomimetic Nano‐Matrix for stem cell anchorage

Abstract

Mesenchymal stem cells (MSCs) have been widely applied in biomedicine due to their ability to differentiate into many different cell types and their ability to synthesize a broad spectrum of growth factors and cytokines that directly and indirectly influence other cells in their vicinity. To guide MSC infiltration to a bone fracture site, we developed a novel self-assembled Nano-Matrix which can be used as an injectable scaffold to repair bone fractures. The Nano-Matrix is formed by Janus base nanotubes (JBNTs) and fibronectin (FN). JBNTs are nucleobase-derived nanotubes mimicking collagen fibers, and FN is one of the cell adhesive glycoproteins which is responsible for cell-extracellular matrix interactions and guides stem cell migration and differentiation to desired cells types. Here, we demonstrated the successful fabrication and characterization of the JBNT/FN Nano-Matrix as well as its excellent bioactivity that encouraged human MSC migration and adhesion. This work lays a solid foundation for using the Nano-Matrix as an injectable approach to improve MSC retention and function during bone fracture healing. K E Y W O R D Sanchorage, fibronectin, Janus-based nanotubes, mesenchymal stem cells, Nano-Matrix

Yeast Cell wall Particle mediated Nanotube-RNA delivery system loaded with miR365 Antagomir for Post-traumatic Osteoarthritis Therapy via Oral Route

“…Nanotubes AAT was synthesized according to the method described previously 18 , 20 . The gene binding ability of AAT was measured using an agarose gel retardation assay as our group described earlier 56 .…”

“…Janus base nanotubes are new class of biomimetic supermolecule nanomaterial which formed from self-assembled of synthetic DNA base analogues (guanine-cytosine motif) 18 . Previous studies have proved that nanotubes have unique chemical and physical properties 19 and can be used to transport small molecule RNA to cells and tissues with low toxicity, excellent biocompatibility and biodegradability 20 .…”

Fibronectin in Fracture Healing: Biological Mechanisms and Regenerative Avenues

“…Their hydrogel managed to efficiently induce osteoblastic differentiation of MC3T3-E1 cells in vitro, and significantly promote fracture healing in vivo (Ao et al, 2020). Another promising biomaterial seems to be the use of FN in combination with nanotubulesmimicking collagen fibers-which is both injectable and can selfassemble (Zhou et al, 2020). The authors report significantly improved human MSC adhesion and migration into the "Nano-Matrix" in vitro (Zhou et al, 2020).…”

“…Another promising biomaterial seems to be the use of FN in combination with nanotubulesmimicking collagen fibers-which is both injectable and can selfassemble (Zhou et al, 2020). The authors report significantly improved human MSC adhesion and migration into the "Nano-Matrix" in vitro (Zhou et al, 2020).…”

Nanomaterials for Protein Delivery in Anticancer Applications

“…Although the emerging biomimetic delivery discussed has shown significant potential, there is still some room to improve (e.g., enhancing immunostimulatory potency) [ 158 ]. The development of novel DNA-based nanotherapeutics, such as Janus-based nanotubes, were proven to be successful and effective in cell penetration in vivo in multiple studies [ 12 , 49 , 159 , 160 ]. They can be applied into the delivery of chemotherapeutic drugs, photosensitizers, imaging probes, small interfering RNA (siRNA), and other therapeutic agents due to their highly desired cellular uptake and pharmacokinetics, when compared to synthetic polymers, such as the used of DNA-inspired nanoparticles (e.g., the nanopieces) [ 160 ].…”