Self-assembled covalent capillary coating of diazoresin/carboxyl fullerene for analysis of proteins by capillary electrophoresis and a comparison with diazoresin/graphene oxide coating

Yu, Bing; Shu, Xi; Cong, Hailin; Chen, Xin; Liu, Huwei; Yuan, Hua; Chi, Ming

doi:10.1016/j.chroma.2016.02.001

Cited by 36 publications

(17 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After being dried under vacuum at room temperature for 12 h, the DR-coated P(S-DVB) microspheres were exposed under 365 nm UV light with an intensity of 350 mW/cm 2 for 15 min. Consequently, DR was covalently linked to the surface of porous P(S-DVB) microspheres [36], and the yield was 95%.…”

Section: Methodsmentioning

confidence: 99%

Preparation of Porous Poly(Styrene-Divinylbenzene) Microspheres and Their Modification with Diazoresin for Mix-Mode HPLC Separations

Cong

et al. 2017

Materials

Self Cite

View full text Add to dashboard Cite

By using the two-step activated swelling method, monodisperse porous poly(styrene-divinylbenzene) (P(S-DVB)) microparticles were successfully synthesized. The influence of porogens, swelling temperatures and crosslinking agents on the porosity of porous microparticles was carefully investigated. Porous P(S-DVB) microparticles were used as a packing material for high performance liquid chromatography (HPLC). Several benzene analogues were effectively separated in a stainless-steel column as short as 75 mm due to the high specific surface area of the porous microparticles. Porous P(S-DVB) microparticles were further sulfonated and subsequently modified with diazoresin (DR) via electrostatic self-assembly and UV (ultraviolet) radiation. After treatment with UV light, the ionic bonding between sulfonated P(S-DVB) and DR was converted into covalent bonding through a unique photochemistry reaction of DR. Depending on the chemical structure of DR and mobile phase composition, the DR-modified P(S-DVB) stationary phase performed different separation mechanisms, including reversed phase (RP) and hydrophilic interactions. Therefore, baseline separations of benzene analogues and organic acids were achieved by using the DR-modified P(S-DVB) particles as packing materials in HPLC. According to the π–π interactional difference between carbon rings of fullerenes and benzene rings of DR, C60 and C70 were also well separated in the HPLC column packed with DR-modified P(S-DVB) particles.

show abstract

Section: Methodsmentioning

confidence: 99%

Preparation of Porous Poly(Styrene-Divinylbenzene) Microspheres and Their Modification with Diazoresin for Mix-Mode HPLC Separations

Cong

et al. 2017

Materials

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently used nanomaterial coatings for capillary electrophoresis separation of proteins are summarized in Table 3. CE-UV Cytochrome C, lysozyme, bovine serum albumin, myoglobin [69] Although nanoparticles applied mainly as permanent coating materials, gold nanoparticles in protein analysis by capillary electrophoresis have also been used both for permanent and dynamic coatings [70]. Yu et al used didodecyldimethylammonium bromide (DDAB) capped gold nanoparticles (AuNPs) to form a stable dynamic capillary wall coating [61] that generated 75% greater reversed EOF compared to DDAB.…”

Section: Nanomaterials Layersmentioning

confidence: 99%

“…Carbon nanomaterials are mainly immobilized onto the capillary surface via covalent bonding [63,66,69]. Bonn and coworkers prepared a chemical capillary coating with fullerenol (derivatized fullerene) covalently bonded to silanized capillary surface [63].…”

Section: Nanomaterials Layersmentioning

confidence: 99%

“…Qu et al applied a covalently coated capillary with graphene oxide and obtained good separation of five chicken egg white proteins [66]. Chi and coworkers used photosensitive diazoresin (DR) as coupling agent to covalently link carboxyl-fullerene to the inner capillary surface [69]. By replacing the toxic and highly moisture sensitive silane coupling agent with DR, the authors presented a promising, environmental friendly and M A N U S C R I P T…”

Section: Nanomaterials Layersmentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in column coatings for capillary electrophoresis of proteins

Hajba

Guttman

2017

TrAC Trends in Analytical Chemistry

121

View full text Add to dashboard Cite

Capillary coatings effectively improve the separation performance of proteins in capillary electrophoresis, mainly by reducing protein adsorption onto the inner capillary wall and by regulating the electroosmotic flow (EOF) to accommodate the separation problem in hand. In the first part of this review the newest trends in dynamic and permanent capillary coatings are summarized and discussed in detail. In the second part the application of nanomaterials as novel capillary coating materials is conversed. Nanomaterials have great potential in capillary coating preparations based on their advantageous properties such as large surface-to-volume ratios and a wide variety of chemistry options. Finally, some future prospective of capillary coatings in the emerging field of proteomics are given.

show abstract

“…But this method may also have an influence on the membrane divalent ion separation efficiency and flux (Mo et al, 2012). In addition, surface carboxyl groups can also be used for surface modification (Jie et al, 2015;Li et al, 2016;Yu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Shielding membrane surface carboxyl groups by covalent-binding graphene oxide to improve anti-fouling property and the simultaneous promotion of flux

Han

Xia

et al. 2016

Water Research

View full text Add to dashboard Cite

a b s t r a c tGraphene oxide (GO) is an excellent material for membrane surface modification. However, little is known about how and to what extent surface functional groups change after GO modification influence membrane anti-fouling properties. Carboxyl is an inherent functional group on polyamide or other similar membranes. Multivalent cations in wastewater secondary effluent can bridge with carboxyls on membrane surfaces and organic foulants, resulting in serious membrane fouling. In this study, carboxyls of a polydopamine (pDA)/1,3,5-benzenetricarbonyl trichloride (TMC) active layer are shielded by covalently-bound GO. The process is mediated by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC)/N-hydroxysuccinimide (NHS). For GO containing low quantities of carboxyls, X-ray photoelectron spectroscopy (XPS) and zeta potential analyzer test results reveal that the carboxyl density decreased by 52.3% compare to the pDA/TMC membrane after GO modification. Fouling experiments shows that the flux only slightly declines in the GO functionalized membrane (19.0%), compared with the pDA/TMC membrane (36.0%) after fouling. In addition, during GO modification process the pDA/TMC active layer also become harder and thinner with the aid of EDC/NHS. So the pure water permeability increases from 56.3 ± 18.2 to 103.7 ± 12.0 LMH/MPa. Our results provide new insights for membrane modification work in water treatment and other related fields.

show abstract

Self-assembled covalent capillary coating of diazoresin/carboxyl fullerene for analysis of proteins by capillary electrophoresis and a comparison with diazoresin/graphene oxide coating

Cited by 36 publications

References 40 publications

Preparation of Porous Poly(Styrene-Divinylbenzene) Microspheres and Their Modification with Diazoresin for Mix-Mode HPLC Separations

Preparation of Porous Poly(Styrene-Divinylbenzene) Microspheres and Their Modification with Diazoresin for Mix-Mode HPLC Separations

Recent advances in column coatings for capillary electrophoresis of proteins

Shielding membrane surface carboxyl groups by covalent-binding graphene oxide to improve anti-fouling property and the simultaneous promotion of flux

Contact Info

Product

Resources

About