Self-Assembled DNA Generated Electric Current Biosensor for HER2 Analysis

Abstract

We have developed a new DNA self-assembly amplification technology that generates electric current for electrochemical biosensing. The new technology was used for detection of human epidermal growth factor receptor 2 (HER2). In our technology, an aptamer was utilized both as a ligand for recognition and as a signal generating reporter. The aptasensor is based on a sandwich format and a DNA primer on a HER2 aptamer initiates auxiliary DNA self-assembled on the electrode to form a long one-dimensional DNA. The resulting DNA is then reacted with molybdate to generate electrochemical current. The sensitivity of the aptasensor with DNA self-assembly was greater than that of the aptasensor without DNA self-assembly due to the extended length of the DNA strand. Aptasensor analysis of HER2 in serum of breast cancer patients and healthy individuals is highly correlated (R = 0.9924) with ELISA measurements, with a p value of 1.37 × 10. The analysis of HER2 in serum (confirmed by ELISA) suggests that HER2 levels in breast cancer patients are much higher than healthy individuals. For HER2 positive patients, the levels are higher than those of HER2 negative patients. After surgery, there is a drop of HER2 levels in serum, suggesting potential clinical applications of the new self-assembled DNA electric current generating biosensor. Unlike proteins, DNA is easily amplifiable. The DNA signal amplification method presented here enables effective current generation, which can find wide range of biomedical applications for protein detection.

Sensitive sandwich-type voltammetric immunosensor for breast cancer biomarker HER2 detection based on gold nanoparticles decorated Cu-MOF and Cu2ZnSnS4 NPs/Pt/g-C3N4 composite

“…In this study, a sandwich-type aptamer and a DNA primer could form a long one-dimensional DNA which was treated with molybdate for generation of electrochemical current. According to the obtained results, the prepared aptasensor for HER2 detection in serum was highly correlated with ELISA measurements [21]. Finally, an aptamer-based colorimetric technique based on AuNPs was developed for HER2 detection.…”

The Signal Amplification in Electrochemical Detection of Chloramphenicol Using Sulfonated Polyaniline‐chitosan Composite as Redox Capacitor

“…Currently, many novel strategies about signal amplification based on functional nanomaterials , enzyme‐signal , DNA self‐assembly and so on have been established for improving sensitivity of electrochemical detection. Among of them, a common method is to introduce a homogeneous chemical reaction coupled with electrode reaction for signal amplification.…”

Impact of menopausal status and HER-2/neu protein on efficacy of EGFR-TKI in EGFR mutant patients with non-small cell lung cancer

“…Combined testing of both EGFR mutation and HER2 protein expression level in peripheral blood may better predict the efficacy of EGFR-TKI on advanced NSCLC. Research data showed that the concentration of HER2 in breast cancer patient's blood elevate to 15-75 ng/mL compared to 2-15 ng/mL in normal individuals 23 . The amount of HER2 in the blood of healthy people is 2-15 ng/mL, which can explain the high expression of HER2 in cancer patients.…”