Self-assembled fibrinogen nanofibers support fibroblast adhesion and prevent E. coli infiltration

Suter, Naiana; Joshi, Arundhati; Wunsch, Timo; Graupner, Nina; Stapelfeldt, Karsten; Radmacher, Manfred; Müssig, Jörg; Brüggemann, Dorothea

doi:10.1016/j.msec.2021.112156

Cited by 13 publications

(34 citation statements)

References 94 publications

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“…6 µm high, mountainous landscape with undulated microtopography (Figure 1c), in agreement with previous studies. [ 43–45 ]…”

Section: Resultsmentioning

confidence: 99%

“…6 μm high, mountainous landscape with undulated microtopography (Figure 1c), in agreement with previous studies. [43][44][45] Static incubation of PRP from healthy donors on the three different surfaces resulted in platelet adsorption and spreading to different degrees. SEM images showed mainly round platelets without or with filopodia on physisorbed and planar fibrinogen surfaces, whereas platelets appeared denser and more spread on fibrinogen nanofibers (Figure 1d-f and Figure S2, Supporting Information).…”

Section: Nanofibers Promote Platelet Adhesion and Spreading On Fibrin...mentioning

confidence: 99%

“…The surface area accessible to platelets for adhesion and spreading thus might well be >10x larger than on the planar scaffold, an estimate which is further supported by the measured increase in surface roughness of nanofiber scaffolds by 15-fold compared to planar. [45] Based on this analysis, we hypothesized that an increased locally accessible surface area due to the mountainous 3D topography directly modulated platelet spreading by offering more immobilized fibrinogen molecules that can be bound by platelet integrins. To test our hypothesis in a more generic setting, we turned toward PDMS micropost arrays as a geometrically welldefined 3D topography model.…”

Section: Nanofibers Promote Platelet Spreading By Locally Increasing ...mentioning

confidence: 99%

“…[43,44] These self-assembled fibrinogen nanofibers resemble the architecture of native fibrin clots, exhibit mechanical properties similar to fibrin upon rehydration, promote fibroblast adhesion, and prevent infiltration with Escherichia coli bacteria. [45] To further explore the potential of self-assembled fibrinogen nanofibers as new scaffold material for the treatment of acute and chronic wounds, we here assessed the interaction of different fibrinogen topographies with human platelets in the absence or presence of plasma proteins. Unlike fibrin which offers only limited tuning of fibril dimensions, [46,47] the salt-induced self-assembly of fibrinogen can be controlled to yield planar 2D films or nanofibrous 3D scaffolds with nearly identical surface chemistries, as previously characterized by circular dichroism and Fourier-Transform Infrared (FTIR) spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

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Nanofiber Topographies Enhance Platelet‐Fibrinogen Scaffold Interactions

Kenny

Stamboroski

Taher

et al. 2022

Adv Healthcare Materials

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The initial contact with blood and its components, including plasma proteins and platelets, directs the body's response to foreign materials. Natural scaffolds of extracellular matrix or fibrin contain fibrils with nanoscale dimensions, but how platelets specifically respond to the topography and architecture of fibrous materials is still incompletely understood. Here, planar and nanofiber scaffolds are fabricated from native fibrinogen to characterize the morphology of adherent platelets and activation markers for phosphatidylserine exposure and α‐granule secretion by confocal fluorescence microscopy and scanning electron microscopy. Different fibrinogen topographies equally support the spreading and α‐granule secretion of washed platelets. In contrast, preincubation of the scaffolds with plasma diminishes platelet spreading on planar fibrinogen surfaces but not on nanofibers. The data show that the enhanced interactions of platelets with nanofibers result from a higher locally accessible surface area, effectively increasing the ligand density for integrin‐mediated responses. Overall, fibrinogen nanofibers direct platelets toward robust adhesion formation and α‐granule secretion while minimizing their procoagulant activity. Similar results on fibrinogen‐coated polydimethylsiloxane substrates with micrometer‐sized 3D features suggest that surface topography could be used more generally to steer blood‐materials interactions on different length scales for enhancing the initial wound healing steps.

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“…6 µm high, mountainous landscape with undulated microtopography (Figure 1c), in agreement with previous studies. [ 43–45 ]…”

Section: Resultsmentioning

confidence: 99%

Section: Nanofibers Promote Platelet Adhesion and Spreading On Fibrin...mentioning

confidence: 99%

Section: Nanofibers Promote Platelet Spreading By Locally Increasing ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nanofiber Topographies Enhance Platelet‐Fibrinogen Scaffold Interactions

Kenny

Stamboroski

Taher

et al. 2022

Adv Healthcare Materials

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“…Despite these conformational changes, no pathogenic amyloid formation was observed, and we could show that a cross-linking treatment with formaldehyde vapor did not affect the secondary structure of self-assembled fibrinogen fibers . So far, we have exclusively used monovalent metal ions to induce fiber assembly of fibrinogen. − Jandt and co-workers previously also postulated that positively charged metal ions like K + and Na + are responsible for fibrinogen fiber formation . Nevertheless, two other studies reported fiber assembly of fibrinogen in buffers containing either 2 mM Ca 2+ or 5 mM Mg 2+ ions .…”

Section: Introductionmentioning

confidence: 82%

Influence of Divalent Metal Ions on the Precipitation of the Plasma Protein Fibrinogen

et al. 2021

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Fibrinogen nanofibers are very attractive biomaterials to mimic the native blood clot architecture. Previously, we reported the self-assembly of fibrinogen nanofibers in the presence of monovalent salts and have now studied how divalent salts influence fibrinogen precipitation. Although the secondary fibrinogen structure was significantly altered with divalent metal ions, morphological analysis revealed exclusively smooth fibrinogen precipitates. In situ monitoring of the surface roughness facilitated predicting the tendency of various salts to form fibrinogen fibers or smooth films. Analysis of the chemical composition revealed that divalent salts were removed from smooth fibrinogen films upon rinsing while monovalent Na + species were still present in fibrinogen fibers. Therefore, we assume that the decisive factor controlling the morphology of fibrinogen precipitates is direct ion−protein contact, which requires disruption of the ion-surrounding hydration shells. We conclude that in fibrinogen aggregates, this mechanism is effective only for monovalent ions, whereas divalent ions are limited to indirect fibrinogen adsorption.

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Antimicrobial Biomaterials Based on Physical and Physicochemical Action

Nowotnick,

Xi,

Jin

et al. 2024

Adv Healthcare Materials

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Developing effective antimicrobial biomaterials is a relevant and fast‐growing field in advanced healthcare materials. Several well‐known (e.g., traditional antibiotics, silver, copper etc.) and newer (e.g., nanostructured, chemical, biomimetic etc.) approaches have been researched and developed in recent years and valuable knowledge has been gained. However, biomaterials associated infections (BAIs) remain a largely unsolved problem and breakthroughs in this area are sparse. Hence, novel high risk and potential high gain approaches are needed to address the important challenge of BAIs. Antibiotic free antimicrobial biomaterials that are largely based on physical action are promising, since they reduce the risk of antibiotic resistance and tolerance. Here, selected examples are reviewed such antimicrobial biomaterials, namely switchable, protein‐based, carbon‐based and bioactive glass, considering microbiological aspects of BAIs. The review shows that antimicrobial biomaterials mainly based on physical action are powerful tools to control microbial growth at biomaterials interfaces. These biomaterials have major clinical and application potential for future antimicrobial healthcare materials without promoting microbial tolerance. It also shows that the antimicrobial action of these materials is based on different complex processes and mechanisms, often on the nanoscale. The review concludes with an outlook and highlights current important research questions in antimicrobial biomaterials.

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Self-assembled fibrinogen nanofibers support fibroblast adhesion and prevent E. coli infiltration

Cited by 13 publications

References 94 publications

Nanofiber Topographies Enhance Platelet‐Fibrinogen Scaffold Interactions

Nanofiber Topographies Enhance Platelet‐Fibrinogen Scaffold Interactions

Influence of Divalent Metal Ions on the Precipitation of the Plasma Protein Fibrinogen

Antimicrobial Biomaterials Based on Physical and Physicochemical Action

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