Self-Assembled Fmoc-Peptides as a Platform for the Formation of Nanostructures and Hydrogels

Orbach, Ron; Adler‐Abramovich, Lihi; Zigerson, Sivan; Mironi‐Harpaz, Iris; Seliktar, Dror; Gazit, Ehud

doi:10.1021/bm900584m

Cited by 311 publications

(297 citation statements)

References 51 publications

(125 reference statements)

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“…Modified dipeptides also could be used as templates for self-assembling nanostructures with tunable biological Journal of Nanomaterials functions [56]. The modified dipeptides containing an Nterminal -amino acid could self-assemble into nanotubes in the solid state and in aqueous solutions [57].…”

Section: Dipeptidementioning

confidence: 99%

Peptide Self-Assembled Nanostructures for Drug Delivery Applications

Fan

Shen

et al. 2017

Journal of Nanomaterials

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Peptide self-assembled nanostructures are very popular in many biomedical applications. Drug delivery is one of the most promising applications among them. The tremendous advantages for peptide self-assembled nanostructures include good biocompatibility, low cost, tunable bioactivity, high drug loading capacities, chemical diversity, specific targeting, and stimuli responsive drug delivery at disease sites. Peptide self-assembled nanostructures such as nanoparticles, nanotubes, nanofibers, and hydrogels have been investigated by many researchers for drug delivery applications. In this review, the underlying mechanisms for the self-assembled nanostructures based on peptides with different types and structures are introduced and discussed. Peptide self-assembled nanostructures associated promising drug delivery applications such as anticancer drug and gene drug delivery are highlighted. Furthermore, peptide self-assembled nanostructures for targeted and stimuli responsive drug delivery applications are also reviewed and discussed.

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Section: Dipeptidementioning

confidence: 99%

Peptide Self-Assembled Nanostructures for Drug Delivery Applications

Fan

Shen

et al. 2017

Journal of Nanomaterials

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“…Both Constructs I and II were prepared using FVC fibers as the template primary layer. These fibers were utilized due to their ability to undergo facile self-assembly, their biocompatibility and their ability to efficiently adhere to biomolecules [39,82]. In general, Fmoc based peptides have been shown to inherently support co-assembly with proteins resulting in supramolecular complexes [83].…”

Section: Development Of Scaffoldsmentioning

confidence: 99%

Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

Romanelli

Knoll

Santora

et al. 2015

Fibers

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Advances in tissue engineering have enabled the ability to design and fabricate biomaterials at the nanoscale that can actively mimic the natural cellular environment of host tissue. Of all tissues, cartilage remains difficult to regenerate due to its avascular nature. Herein we have developed two new hybrid polypeptide-glycosaminoglycan microfibrous scaffold constructs and compared their abilities to stimulate cell adhesion, proliferation, sulfated proteoglycan synthesis and soluble collagen synthesis when seeded with chondrocytes. Both constructs were designed utilizing self-assembled Fmoc-protected valyl cetylamide nanofibrous templates. The peptide components of the constructs were varied. For Construct I a short segment of dentin sialophosphoprotein followed by Type I collagen were attached to the templates using the layer-by-layer approach. For Construct II, a short peptide segment derived from the integrin subunit of Type II collagen binding protein expressed by chondrocytes was attached to the templates followed by Type II collagen. To both constructs, we then attached the natural polymer N-acetyl glucosamine, chitosan. Subsequently, the glycosaminoglycan chondroitin sulfate was then attached as the final layer. The scaffolds were characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), atomic force microscopy and scanning electron microscopy. In vitro culture studies were carried out in the presence of chondrocyte cells for OPEN ACCESSFibers 2015, 3 266 both scaffolds and growth morphology was determined through optical microscopy and scanning electron microscopy taken at different magnifications at various days of culture. Cell proliferation studies indicated that while both constructs were biocompatible and supported the growth and adhesion of chondrocytes, Construct II stimulated cell adhesion at higher rates and resulted in the formation of three dimensional cell-scaffold matrices within 24 h. Proteoglycan synthesis, a hallmark of chondrocyte cell differentiation, was also higher for Construct II compared to Construct I. Soluble collagen synthesis was also found to be higher for Construct II. The results of the above studies suggest that scaffolds designed from Construct II be superior for potential applications in cartilage tissue regeneration. The peptide components of the constructs play an important role not only in the mechanical properties in developing the scaffolds but also control cell adhesion, collagen synthesis and proteoglycan synthesis capabilities.

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“…However, doping of fmoc-RGD into a system predominantly consisting of fmoc-FF results in the disruption of the ordering of the system but an increase in the biofunctionality [38]. The presentation of RGD has also been observed to be a function of the aromatic sidechains flanking the epitope, where fmoc-RDGF has a greater bioavailability than fmoc-FRGD, possibly through increased rigidity provided by the aromatic residue at the C terminal [45]. When the RGD peptide was included as a pendant molecule on an oligopeptide, the biological response was increased without disrupting the mechanism of assembly as the pendant signal was not integral to the assembly process [20].…”

Section: Functionalisation Of Peptide Materialsmentioning

confidence: 99%

Self-Assembled Peptides: Characterisation and In Vivo Response

Nisbet

Williams

2012

Biointerphases

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The fabrication of tissue engineering scaffolds is a well-established field that has gained recent prominence for the in vivo repair of a variety of tissue types. Recently, increasing levels of sophistication have been engineered into adjuvant scaffolds facilitating the concomitant presentation of a variety of stimuli (both physical and biochemical) to create a range of favourable cellular microenvironments. It is here that self-assembling peptide scaffolds have shown considerable promise as functional biomaterials, as they are not only formed from peptides that are physiologically relevant, but through molecular recognition can offer synergy between the presentation of biochemical and physio-chemical cues. This is achieved through the utilisation of a unique, highly ordered, nano- to microscale 3-D morphology to deliver mechanical and topographical properties to improve, augment or replace physiological function. Here, we will review the structures and forces underpinning the formation of self-assembling scaffolds, and their application in vivo for a variety of tissue types.

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Self-Assembled Fmoc-Peptides as a Platform for the Formation of Nanostructures and Hydrogels

Cited by 311 publications

References 51 publications

Peptide Self-Assembled Nanostructures for Drug Delivery Applications

Peptide Self-Assembled Nanostructures for Drug Delivery Applications

Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

Self-Assembled Peptides: Characterisation and In Vivo Response

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