Self-assembled lysozyme/carboxymethylcellulose nanogels for delivery of methotrexate

Li, Zhenshun; Xu, Wei; Zhang, Chunlan; Chen, Yijie; Li, Bin

doi:10.1016/j.ijbiomac.2015.01.033

Cited by 45 publications

(9 citation statements)

References 33 publications

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“…Similar to albumin-based nanocarriers, several lysozyme (Lys) nanovehicles have been developed for the delivery of drug cargos (Li et al., 2015 ; Lin et al., 2015 ; Mahanta et al., 2015 ). Lys has 129 amino acids with a molecular weight of 14.4 kDa (Canfield, 1963 ).…”

Section: Introductionmentioning

confidence: 99%

“…Its antibacterial and anticancer activities have also been elucidated in recent years (Aminlari et al., 2014 ; Mahanta et al., 2015 ). Self-assembled Lys nanogels, alone or in combination with other materials, have been developed as drug delivery systems (Li et al., 2015 ; Lin et al., 2015 ). In this study, D-α-tocopherol succinate (αTS) was chemically conjugated to Lys as a hydrophobic residue.…”

Section: Introductionmentioning

confidence: 99%

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An α-tocopheryl succinate enzyme-based nanoassembly for cancer imaging and therapy

Lee

Cho

2018

Drug Delivery

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Nanoassembly (NA) based on a D-α-tocopherol succinate (αTS) conjugated lysozyme (Lys) (Lys-αTS) was fabricated for tumor-selective delivery of curcumin (CUR) for breast cancer therapy. Lys and αTS were used as a biocompatible enzyme and a hydrophobic residue, respectively, for the preparation of nanocarriers in this study. Compared with CUR-loaded cross-linked Lys (c-Lys/CUR) NA, Lys-αTS/CUR NA exhibited a smaller hydrodynamic size (213 nm mean diameter), a narrower size distribution, and a more spherical shape. Sustained drug release was observed from the Lys-αTS/CUR NA for five days at a normal physiological pH (pH 7.4). The developed Lys-αTS/CUR NA showed enhanced cellular accumulation, antiproliferative effects, and apoptotic efficacies in MDA-MB-231 human breast adenocarcinoma cells. According to the results of optical imaging test in the MDA-MB-231 tumor-bearing mouse models, the Lys-αTS/CUR NA-injected group exhibited a more tumor-selective accumulation pattern, rather than being distributed in the normal tissues and organs. The observed tumor targetability of Lys-αTS/CUR was further studied, which revealed improved in vivo anticancer activities (better inhibition of tumor growth and induction of apoptosis in the tumor tissue) after an intravenous administration in the MDA-MB-231 tumor-bearing mouse models. All these results indicate that the newly developed enzyme-based nanocarrier, the Lys-αTS NA, can be a promising candidate for the therapy of breast cancers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An α-tocopheryl succinate enzyme-based nanoassembly for cancer imaging and therapy

Lee

Cho

2018

Drug Delivery

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“…Pyrene as a strongly hydrophobic fluorescent probe could be utilized to track the expose of hydrophobic regions of protein molecules. It can spontaneously move to the hydrophobic region of the solution when exposed to the aqueous phase due to its low aqueous solubility (Li et al., 2015). The fluorescence spectrum of pyrene has five emission peaks with excitation at 335 nm.…”

Section: Resultsmentioning

confidence: 99%

The interaction mechanism between liposome and whey protein: Effect of liposomal vesicles concentration

Yang

Pan

et al. 2021

Journal of Food Science

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The interaction mechanism between liposomes (Lips) and whey protein isolates (WPI) with different mass ratios was explored in this paper. After binding with different concentration of Lips, the changes in hydrophilic and hydrophobic regions of WPI were investigated with fluorescein isothiocyanate (FITC) and pyrene fluorescence probes. The spatial structure changes of WPI were further characterized by differential scanning calorimetry, Fourier transform infrared spectroscopy, and circular dichroism. The results indicated that the structure of WPI was changed due to binding with Lips in hydrophilic and hydrophobic groups. The binding process might result in the migration, recombination, and alignment of WPI and Lip groups. Moreover, the oil‐water interfacial tension with WPI decreased from 9.20 mN/m to 3.29 mN/m upon increasing the Lip‐to‐WPI ratio. This work suggests that the physiochemical properties of Lip–WPI complexes could be manipulated by adjusting the Lip‐to‐WPI ratio. This study shed some light on the mechanism explanation of the WPI structural changes due to the interaction with Lips during food processing.

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“…Therefore, the EE and LC percentages of MTX in CDs@NGs were 71% and 34%, respectively. Usually, less LC values have been reported for similarly structured polymeric nanoparticles …”

Section: Resultsmentioning

confidence: 99%

Glutathione and pH‐responsive fluorescent nanogels for cell imaging and targeted methotrexate delivery

Ghorbani

Mahmoodzadeh

Jannat

et al. 2019

Polymers for Advanced Techs

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Cancer is one of the health problems that lead to death in the world, and nanotechnology was shown to have a unique potential to improve the therapeutic efficacy of anticancer agents. The nanosized drug delivery systems (DDSs) have been offered for targeting tumor tissue because of enhanced drug bioavailability and long circulation time. In this context, we reported a facial approach to prepare a novel pH and glutathione‐responsive nanogel. After that, the nanocarriers coupled with highly fluorescent quantum dots were developed. Then methotrexate (MTX) was loaded into and on the surface of nanogels by ionic interaction so that the triggered MTX release ability of the synthesized nanocarriers was verified through the assessment of in vitro drug release at simulated tumor tissue condition. The improved efficiency of the developed nanogels and their targeted performance via conjugation of MTX (as target ligand of folate receptors) were investigated through the various cell cytotoxicity studies such as 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, 4′6‐diamidino‐2‐phenylindole (DAPI) staining, and flow cytometry. The results of various cell cytotoxicity studies concluded that the developed smart nanogels have many promising abilities for the targeted MTX delivery to cancer tissues.

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Self-assembled lysozyme/carboxymethylcellulose nanogels for delivery of methotrexate

Cited by 45 publications

References 33 publications

An α-tocopheryl succinate enzyme-based nanoassembly for cancer imaging and therapy

An α-tocopheryl succinate enzyme-based nanoassembly for cancer imaging and therapy

The interaction mechanism between liposome and whey protein: Effect of liposomal vesicles concentration

Glutathione and pH‐responsive fluorescent nanogels for cell imaging and targeted methotrexate delivery

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