Self-assembled mirror DNA nanostructures for tumor-specific delivery of anticancer drugs

Antibody-Conjugated, DNA-Based Nanocarriers Intercalated with Doxorubicin Eliminate Myofibroblasts in Explants of Human Lens Tissue

“…G8:3DNA:Dox specifically killed Myo/Nog cells in human lens explant cultures, indicating that doxorubicin escaped the endosome. This result is consistent with previous studies demonstrating that: 1) four-layered DNA dendrimers conjugated with an antibody to intercellular adhesion molecule-1 are internalized into endosomes and permit the escape of endosomal contents into the cytoplasm (Muro, 2014), and 2) doxorubicin is released more rapidly from DNA at an acidic pH (Kim et al, 2016).…”

“…1B. Release of doxorubicin from DNA nanoparticles is enhanced at acid pH (Kim et al, 2016). Demonstration of internalization into acidic compartments within Myo/Nog cells was carried out by simultaneous incubation of explants of human anterior lens tissue with 3DNA nanocarriers conjugated with the G8 mAb and the fluorochrome Cy3 (G8:3DNA:Cy3) and the LysoSensor green dye that only fluoresces at acidic pH (#pH 5).…”

DNA Trojan Horses: Self‐Assembled Floxuridine‐Containing DNA Polyhedra for Cancer Therapy

“…[8] Importantly,DNA exhibits excellent properties of molecular recognition and programmable self-assembly, [9] by which it can be employed as building blocks for constructing well-defined nanostructures and nanodevices. [11] Most of them rely on intercalating small molecule therapeutics into DNA duplexes. [11] Most of them rely on intercalating small molecule therapeutics into DNA duplexes.…”

“…[10] To date,various one-, two-, and three-dimensional (1D,2 D, and 3D) DNAn anostructures have been engineered for drug delivery. [11,12] Meanwhile,m any others realize the drug delivery by creating am icellar environment within the DNAn anostructures,w hich enables the encapsulation and release of hydrophobic antitumor agents. [11,12] Meanwhile,m any others realize the drug delivery by creating am icellar environment within the DNAn anostructures,w hich enables the encapsulation and release of hydrophobic antitumor agents.…”

“…[11] Most of them rely on intercalating small molecule therapeutics into DNA duplexes. [11,12] Meanwhile,m any others realize the drug delivery by creating am icellar environment within the DNAn anostructures,w hich enables the encapsulation and release of hydrophobic antitumor agents. [13] Despite the artful design for these drug carriers,itisdifficult to precisely control the formulation through intercalating or encapsulating drug molecules inside the DNAn anostructures.M oreover,d rug intercalation and DNAmodification may intrinsically change the structural features of nucleic acids,w hich may arouse an immune response.Alternatively,asan important category of anticancer drugs,m any nucleoside analogues,s uch as floxuridine,d ecitabine,g emcitabine,c an be integrated into the nucleic acid strand by replacing the similar nucleoside units with no influence on their capability for molecular recognition.…”