Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies

Faucheux, Nathalie; Schweiss, Ruediger; Lützow, Karola; Werner, Carsten; Groth, Thomas

doi:10.1016/j.biomaterials.2003.09.069

Cited by 685 publications

(670 citation statements)

References 49 publications

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“…Previous studies reported that very hydrophilic substrates (θ < 35°) inhibit the adsorption of serum proteins, causing lower spreading and attachment of human fibroblasts in contrast to the surfaces with moderately wettable surfaces (i.e., θ = 48°-62°). [29] Similar to our findings, multiple studies report weak cell attachment on TNT. These observations are also in line with the cell behavior observed on other ordered regular nanofeatures such as nanoposts [61] and nanopits.…”

Section: Cell Adhesionsupporting

confidence: 91%

“…However, the majority of chemical methods modify not only topography, but also other surface features such as chemical composition, [25] wettability, [26] crystallinity, [27] and adsorption ability. [28] These surface observables can also modulate cell behavior [27,[29][30][31] (for more information about surface modification strategies and cellular recognition of these surfaces see the review papers [32,33] ) and it is difficult to distinguish which factor leads to the observed cell response.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

Zhukova

Hiepen

Knaus

et al. 2017

Adv Healthcare Materials

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Surface structuring of titanium-based implants is known to modulate the behavior of adherent cells, but the influence of different nanotopographies is poorly understood. The aim is to investigate preosteoblast proliferation, adhesion, morphology, and migration on surfaces with similar surface chemistry but distinct nanotopographical features. Sonochemical treatment and anodic oxidation are employed to fabricate disordered, mesoporous titania (TMS) and ordered titania nanotubular (TNT) topographies on titanium, respectively. Morphological evaluation reveals that cells are polygonal and well-spread on TMS, but display an elongated, fibroblast-like morphology on TNT surfaces, while they are much flatter on glass. Both nanostructured surfaces impair cell adhesion, but TMS is more favorable for cell growth due to its support of cell attachment and spreading in contrast to TNT. A quantitative wound healing assay in combination with live-cell imaging reveals that cell migration on TMS surfaces has a more collective character than on other surfaces, probably due to a closer proximity between neighboring migrating cells on TMS. The results indicate distinctly different cell adhesion and migration on ordered and disordered titania nanotopographies, providing important information that can be used in optimizing titanium-based scaffold design to foster bone tissue growth and repair while allowing for the encapsulation of drugs into porous titania layer

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Section: Cell Adhesionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

Zhukova

Hiepen

Knaus

et al. 2017

Adv Healthcare Materials

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“…[52,53] To study whether the initial amount of adsorbed fibronectin on PLLA/ZnO was due to differences in the initial superficial charges of the surfaces, we measured the Z-potential. However, similar values were obtained for both surfaces, -30.20 ± 0.75 mV -PLLA and -33.07 ± 3.65mV -PLLA/ZnO, which disregards the possible effect of surface charge on the higher protein adsorption measured on the nanocomposite.…”

Section: Figure 4amentioning

confidence: 99%

PLLA/ZnO nanocomposites: Dynamic surfaces to harness cell differentiation

Trujillo

Lizundia

Vilas

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Graphical abstract2 Highlights  Biodegradable poly(L-lactide) matrix loaded with ZnO nanorods. Dynamic presentation of nanorods triggered by PLLA degradation. Changes in surface properties as a function of time control cell behaviour. Nanorods exposure promotes cell differentiation.3 Keywords: PLLA, ZnO nanoparticle, C2C12 myoblast, nanocomposite, cell differentiation AbstractThis work investigates the effect of the sequential availability of ZnO nanoparticles, (nanorods of ~ 20 nm) loaded within a degradable poly(lactic acid) (PLLA) matrix, in cell differentiation. The system constitutes a dynamic surface, in which nanoparticles are exposed as the polymer matrix degrades. ZnO nanoparticles were loaded into PLLA and the system was measured at different time points to characterise the time evolution of the physicochemical properties, including wettability and thermal properties. The micro and nanostructure were also investigated using AFM, SEM and TEM images. Cellular experiments with C2C12 myoblasts show that cell differentiation was significantly enhanced on ZnO nanoparticles -loaded PLLA, as the polymer degrades and the availability of nanoparticles become more apparent, whereas the release of zinc within the culture medium was negligible. Our results suggest PLLA/ZnO nanocomposites can be used as a dynamic system where nanoparticles are exposed during degradation, activating the material surface and driving cell differentiation.4

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“…28 The presence of acid functional groups on a surface is known to promote cell attachment. [37][38][39] If the cells are adhered too well, they are less likely to get transferred to the wound bed. 28 Therefore, we investigated the transfer efficiency of surfaces with various concentrations of acid functionalities.…”

Section: Discussionmentioning

confidence: 99%

Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency

Brown

Low

Mariappan

et al. 2014

Tissue Engineering Part A

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Extensive damage to the limbal region of the cornea leads to a severe form of corneal blindness termed as limbal stem cell deficiency (LSCD). Whereas most cases of corneal opacity can be treated with full thickness corneal transplants, LSCD requires stem cell transplantation for successful ocular surface reconstruction. Current treatments for LSCD using limbal stem cell transplantation involve the use of murine NIH 3T3 cells and human amniotic membranes as culture substrates, which pose the threat of transmission of animal-derived pathogens and donor tissue-derived cryptic infections. In this study, we aimed to produce surface modified therapeutic contact lenses for the culture and delivery of corneal epithelial cells for the treatment of LSCD. This approach avoids the possibility of suture-related complications and is completely synthetic. We used plasma polymerization to deposit acid functional groups onto the lenses at various concentrations. Each surface was tested for its suitability to promote corneal epithelial cell adhesion, proliferation, retention of stem cells, and differentiation and found that acid-based chemistries promoted better cell adhesion and proliferation. We also found that the lenses coated with a higher percentage of acid functional groups resulted in a higher number of cells transferred onto the corneal wound bed in rabbit models of LSCD. Immunohistochemistry of the recipient cornea confirmed the presence of autologous, transplanted 5-bromo-2¢-deoxyuridine (BrdU)-labeled cells. Hematoxylin staining has also revealed the presence of a stratified epithelium at 26 days post-transplantation. This study provides the first evidence for in vivo transfer and survival of cells transplanted from a contact lens to the wounded corneal surface. It also proposes the possibility of using plasma polymer-coated contact lenses with high acid functional groups as substrates for the culture and transfer of limbal cells in the treatment of LSCD.

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Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies

Cited by 685 publications

References 49 publications

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

PLLA/ZnO nanocomposites: Dynamic surfaces to harness cell differentiation

Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency

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