Self-assembled nanoparticles of hydrophobically-modified polysaccharide bearing vitamin H as a targeted anti-cancer drug delivery system

Na, Kun; Lee, Tae Bum; Park, Keun-Hong; Shin, Eun Jung; Lee, Yong-Bok; Choi, Hoo‐Kyun

doi:10.1016/s0928-0987(02)00257-9

Cited by 205 publications

(73 citation statements)

References 20 publications

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“…Surface receptors for vitamin H have usually a higher concentration in tumors than in normal tissues, since cancer cells require an extra amount of this vitamin due to their fast proliferation [492]. Taheri et al [493,494] developed human serum albumin (HSA) nanoparticles for the targeted delivery of methotrexate, using biotin as targeting ligand.…”

Section: Biotin-based Targetingmentioning

confidence: 99%

Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy

Pérez-Herrero

Fernández‐Medarde

2015

European Journal of Pharmaceutics and Biopharmaceutics

1,465

754

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Cancer is the second worldwide cause of death, exceeded only by cardiovascular diseases. It is characterized by uncontrolled cell proliferation and an absence of cell death that, except for hematological cancers, generates an abnormal cell mass or tumor. This primary tumor grows thanks to new vasculatization and, in time, adquires metastatic potential and spreads to other body sites, which causes metastasis and finally death. Cancer is caused by damage or mutations in the genetic material of the cells due to environmental or inherited factors. While surgery and radiotherapy are the primary treatment used for local and non-metastatic cancers, anti-cancer drugs (chemotherapy, hormone and biological therapies) are the choice currently used in metastasic cancers. Chemotherapy is based on the inhibition of the division of rapidly growing cells, which is a characteristic of the cancerous cells, but unfortunately, it also affects normal cells with fast proliferation rates, like the hair follicles, bone marrow and gastrointestinal tract cells, generating the characteristic side effects of chemotherapy. The indiscriminate destruction of normal cells, the toxicity of conventional chemotherapeutic Código de campo cambiado

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Section: Biotin-based Targetingmentioning

confidence: 99%

Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy

Pérez-Herrero

Fernández‐Medarde

2015

European Journal of Pharmaceutics and Biopharmaceutics

1,465

754

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“…The self-assembled NPs, consisting of a hydrophobic core and hydrophilic shell, have been recognized as a promising drug carrier, since their hydrophobic domain can encapsulate various hydrophobic drugs. 5 In recent years, many studies have been carried out to investigate the synthesis and the application of pullulan-based self-assembled NPs as drug delivery systems, [6][7][8][9][10][11][12] among which, cholesterol-bearing pullulan (CHP), synthesized by the reaction between cholesteryl N-(6-isocynatohexyl)carbamate and pullulan using 1,6-hexyl diisocyanate linkages, has attracted much attention. CHP can form stable hydrogel NPs by self-assembly, 13,14 and can then form complexes with various drugs and proteins by hydrophobic interaction and release them upon exposure to specific proteins or cyclodextrin.…”

Section: Introductionmentioning

confidence: 99%

Cellular uptake mechanism and intracellular fate of hydrophobically modified pullulan nanoparticles

Jiang¹,

Li²,

Liu³

et al. 2013

IJN

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Abstract:The cellular uptake mechanism and intracellular fate of self-assembled nanoparticles (NPs) of cholesterol-modified pullulan (CHSP) by human hepatocellular carcinoma (HepG2) cells were investigated. Covalent conjugation with fluorescein isothiocyanate (FITC) yielded stably labeled CHSP (FITC-CHSP), which was successfully formulated into NPs (mean particle size 63.0 ± 1.9 nm) by dialysis. A cytotoxicity assay clearly indicated that the CHSP NPs did not show significant toxicity in HepG2 cells. The effects of NP concentration, incubation time, and temperature on the cellular uptake of the NPs were systematically evaluated by fluorometry, and the results suggested that cellular uptake of the NPs was concentration-, time-, and temperature-dependent. In vitro experiments with endocytic inhibitors revealed that clathrin-mediated endocytosis and macropinocytosis were involved in the internalization of CHSP NPs. The intracellular trafficking study demonstrated that CHSP NPs were entrapped in the lysosomes at 1 hour after incubation; colocalization of NPs with either the Golgi apparatus or the endoplasmic reticula was not observed during the entire course of the study. These results suggested that the CHSP NPs may serve as a versatile carrier for intracellular delivery of therapeutic agents.

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“…Biodistribution of self-aggregates (300 nm in diameter) was evaluated using tissues obtained from tumor-bearing mice, to which self-aggregates were systemically administered via the tail vein. Na et al (Na et al, 2003) introduced vitamin H to pullulan acetate and prepared corresponding self-assembled nanoparticles (~100 nm) in order to improve their cancertargeting activity and internalization. Three samples of biotinylated pullulan acetate, comprising 7, 20 and 39 vitamin H groups per 100 anhydroglucose units, were synthesized.…”

Section: Cyclic Hydrophobic Moleculesmentioning

confidence: 99%

“…A novel amphiphilic copolymer (dextran-g-polyethyleneglycol alkyl ether) was synthesized which resulted in polymeric micelle formation, encapsulating cyclosporine in the hydrophobic core and providing a hydrophilic corona (Na et al, 2003;Francis et al, 2003).…”

Section: Medical Applications Of Polysaccharide-based Nanoparticlesmentioning

confidence: 99%

Nanoparticles Based on Modified Polysaccharides

Namazi¹,

Fathi²,

Heydari³

2012

The Delivery of Nanoparticles

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Self-assembled nanoparticles of hydrophobically-modified polysaccharide bearing vitamin H as a targeted anti-cancer drug delivery system

Cited by 205 publications

References 20 publications

Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy

Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy

Cellular uptake mechanism and intracellular fate of hydrophobically modified pullulan nanoparticles

Nanoparticles Based on Modified Polysaccharides

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