Self-assembled particles of an elastin-like polymer as vehicles for controlled drug release

Herrero-Vanrell, Rocı́o; Rincón, A.C.; Alonso, Matilde; Reboto, Virginia; Molina‐Martínez, Irene T.; Rodríguez‐Cabello, José Carlos

doi:10.1016/j.jconrel.2004.10.001

Cited by 222 publications

(159 citation statements)

References 20 publications

Supporting

Mentioning

156

Contrasting

Unclassified

Order By: Relevance

“…Surfaces coated with an ELP fused to the RGD or fibronectin CS5 cell binding sequence also retain an ability to support in vitro endothelial cell adhesion and spreading. [36] Other applications of ELP, including entrapment of small molecules such as dexamethasone, [37] have also been investigated and are elsewhere reviewed. [38] The primary objective of this study was to create a fusion protein between an ELP and sTNFRII that would retain the ELP inverse phase transition behavior and sTNFRII domain bioactivity.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of a thermally-responsive tumor necrosis factor antagonist

Shamji

Chen

Friedman

et al. 2008

Journal of Controlled Release

View full text Add to dashboard Cite

Numerous antagonists of tumor necrosis factor alpha (TNFα) have been developed to attenuate inflammation and accompanying pain in many disease processes. Soluble TNF receptor type II (sTNFRII) is one such antagonist that sequesters TNFα away from target receptors and attenuates its activity. Systemic delivery of soluble TNF receptors or other antagonists may have deleterious side effects associated with immune suppression, so that strategies for locally targeted drug delivery are of interest. Elastin-like polypeptides (ELPs) are biopolymers capable of in situ drug depot formation through thermally-driven supramolecular complexes at physiological temperatures. A recombinant fusion protein between ELP and sTNFRII was designed and evaluated for retention of bivalent functionality. Thermal sensitivity was observed by formation of supramolecular submicronsized particles at 32°C, with gradual resolubilization from the depot observed at physiological temperatures. In vitro refolding of the sTNFRII domain was required and the purified product exhibited an equilibrium dissociation constant for interacting with TNFα that was seven-fold higher than free sTNFRII. Furthermore, anti-TNF activity was observed in inhibiting TNFα-mediated cytotoxicity in the murine L929 fibrosarcoma assay. Potential advantages of this ELP-sTNFRII fusion protein as an anti-TNFa drug depot include facility of injection, in situ depot formation, low endotoxin content, and functionality against TNFα.

show abstract

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of a thermally-responsive tumor necrosis factor antagonist

Shamji

Chen

Friedman

et al. 2008

Journal of Controlled Release

View full text Add to dashboard Cite

show abstract

“…As an example, thermoresponsive properties of elastin-like polymers have been exploited to obtain micro-and nanoparticles for the controlled release of dexamethasone phosphate in order to promote osteogenesis [12]. In 2008, some promising evidences were provided about self-assembling of particles suitable for drug release purposes, based on an elastin-like polypeptide mimicking the native alternating structure of tropoelastin [13].…”

Section: Human Elastin-like Polypeptidesmentioning

confidence: 99%

The potential of recombinant human elastin-like polypeptides for drug delivery

Ciofani¹,

Genchi²,

Mattoli³

et al. 2014

Expert Opinion on Drug Delivery

View full text Add to dashboard Cite

Mimicking the structure of natural proteins by recombinant biopolymers is a useful approach for the development of novel bioactive biomaterials with desired properties, that help elucidate molecular interactions in biological systems and elaborate strategies for tissue engineering and drug delivery purposes. Structurally based on elastin repeated motifs, recombinant human elastin-like polypeptides (HELPs) represent excellent examples of bio-inspired polymers proposed for tissue engineering, and recently exploited also for drug delivery applications. This Editorial reports on the latest advances in the research on HELP biopolymers for drug delivery and targeting applications. The main findings will be summarized with emphasis on the ‘smart’ properties of HELPs, which render this class of biopolymers particularly interesting in the whole biomedicine field. Considerations about further improvements of the current HELP-based systems will be provided, and a demonstration of the huge potential of HELPs in becoming leading material for drug delivery will be attempted

show abstract

“…The authors thus showed that genetically engineered ELP carriers can provide a new way to thermally target specific oncogene inhibitors to solid tumors. Herrero-Vanrell et al (2005) have studied the selfassembled Poly(VPAVG) micro-and nanoparticles as vehicles for the controlled release of Dexamethasone phosphate (DMP). Poly (VPAVG) was prepared as stable particles with a size 53 mm above its transition temperature (30 C).…”

Section: Anticancer Drug Targeting Applications Of Elpsmentioning

confidence: 99%

Elastin-like polypeptides and their applications in anticancer drug delivery systems: a review

Saxena

Nanjan

2013

Drug Delivery

View full text Add to dashboard Cite

Elastin-like polypeptides (ELPs) are large molecular weight biopolymers. They have been widely studied as macromolecular carriers for targeted delivery of drugs. The aim of the present article is to review the available information on ELPs (including our recent investigations), their properties, drug delivery applications to tumor sites and future perspectives. This review also provides information on the use of short synthetic ELPs for making ELP-drug conjugates, for targeted delivery of anticancer drugs. In the present review we also focus on the point that short ELPs can also be used for targeting anticancer drugs to tumor sites as they behave similar to long ELPs regarding their capacity to undergo inverse temperature transition (ITT) behavior.

show abstract

Self-assembled particles of an elastin-like polymer as vehicles for controlled drug release

Cited by 222 publications

References 20 publications

Synthesis and characterization of a thermally-responsive tumor necrosis factor antagonist

Synthesis and characterization of a thermally-responsive tumor necrosis factor antagonist

The potential of recombinant human elastin-like polypeptides for drug delivery

Elastin-like polypeptides and their applications in anticancer drug delivery systems: a review

Contact Info

Product

Resources

About